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The map label for this gene is tpiA

Identifier: 169629861

GI number: 169629861

Start: 2824090

End: 2824875

Strand: Reverse

Name: tpiA

Synonym: MAB_2777c

Alternate gene names: 169629861

Gene position: 2824875-2824090 (Counterclockwise)

Preceding gene: 169629862

Following gene: 169629859

Centisome position: 55.75

GC content: 64.63

Gene sequence:

>786_bases
ATGTCGCGTAAGCCGTTGATCGCCGGCAACTGGAAGATGAACCTCAATCATTTCGAGGCCATCGCTTTGGTGCAGAAGAT
CGCGTTCTCGTTGCCGGACAAGTACTTCGACAAGGTGGACGTGACGGTCATTCCGCCGTTCACCGATATCCGCAGCGTGC
AGACCCTGGTCGACGGTGACAAGTTACGCCTTACCTACGGCGCACAGGACCTGTCCGTATATGACTCGGGCGCGTACACC
GGTGAGGTCAGCGGCGCCTTCCTGGCCAAGCTGGGTGTCACCTATGTCGTTGTCGGGCACTCCGAGCGCCGCCAGTATCA
CGGCGAGGACGACGCGCTGGTGGCGGCCAAGGCGGCCGCGGCCCTCAAACACGGCCTCACACCGATCGTGTGTATCGGTG
AGGCACTTGACATCCGGGAAGCCGGAGATCACGTCCAGTACAACGTGAACTCCTTGCGTGGGTCACTGGCCGGCCTCAGC
GCCGAACAGGTGGGCAAGGTGGTTATCGCCTACGAGCCGGTGTGGGCCATCGGCACCGGGCGGGTGGCCAGCGCCGCCGA
CGCGCAGGAAGTGTGCGCCGCGATCCGCGCCGAGTTGGCGCAAATCGCGAACGCCGACGTGGCGGGGTCTGTCCGGGTCT
TGTATGGCGGCTCGGCCAACGCCAAGAATGTCGGCGAGATCGTTGCGCAGGAGGATGTCGACGGAGCGTTGGTCGGTGGC
GCCTCGCTGGACGGCGAGCAGTTCGCCCAGATGTCGGCGATCGCCGCGGGCGGTCCGCTGCTTTAG

Upstream 100 bases:

>100_bases
GTTTCTCGCACATCTCCACCGGTGGTGGTGCATCCCTGGAATACCTTGAAGGCAAGGTGCTTCCGGGTATCGACGTTCTC
GAGGACTAGAGGAGTTCCGT

Downstream 100 bases:

>100_bases
CCGGGATCTACGGCAGATTGCGTAGATGCGCGCCGCGCGCCAACACTCCGTCGAGGGCGGTGAGGAAGGCGGGCAGGTTC
GCGGTATCGCTGGAAGCCGT

Product: triosephosphate isomerase (TpiA)

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSRKPLIAGNWKMNLNHFEAIALVQKIAFSLPDKYFDKVDVTVIPPFTDIRSVQTLVDGDKLRLTYGAQDLSVYDSGAYT
GEVSGAFLAKLGVTYVVVGHSERRQYHGEDDALVAAKAAAALKHGLTPIVCIGEALDIREAGDHVQYNVNSLRGSLAGLS
AEQVGKVVIAYEPVWAIGTGRVASAADAQEVCAAIRAELAQIANADVAGSVRVLYGGSANAKNVGEIVAQEDVDGALVGG
ASLDGEQFAQMSAIAAGGPLL

Sequences:

>Translated_261_residues
MSRKPLIAGNWKMNLNHFEAIALVQKIAFSLPDKYFDKVDVTVIPPFTDIRSVQTLVDGDKLRLTYGAQDLSVYDSGAYT
GEVSGAFLAKLGVTYVVVGHSERRQYHGEDDALVAAKAAAALKHGLTPIVCIGEALDIREAGDHVQYNVNSLRGSLAGLS
AEQVGKVVIAYEPVWAIGTGRVASAADAQEVCAAIRAELAQIANADVAGSVRVLYGGSANAKNVGEIVAQEDVDGALVGG
ASLDGEQFAQMSAIAAGGPLL
>Mature_260_residues
SRKPLIAGNWKMNLNHFEAIALVQKIAFSLPDKYFDKVDVTVIPPFTDIRSVQTLVDGDKLRLTYGAQDLSVYDSGAYTG
EVSGAFLAKLGVTYVVVGHSERRQYHGEDDALVAAKAAAALKHGLTPIVCIGEALDIREAGDHVQYNVNSLRGSLAGLSA
EQVGKVVIAYEPVWAIGTGRVASAADAQEVCAAIRAELAQIANADVAGSVRVLYGGSANAKNVGEIVAQEDVDGALVGGA
SLDGEQFAQMSAIAAGGPLL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=245, Percent_Identity=40.4081632653061, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=40.650406504065, Blast_Score=158, Evalue=4e-39,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=42.5101214574899, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=244, Percent_Identity=42.2131147540984, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=250, Percent_Identity=40, Blast_Score=158, Evalue=6e-40,
Organism=Drosophila melanogaster, GI28572004, Length=250, Percent_Identity=41.2, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28572008, Length=250, Percent_Identity=41.2, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28572006, Length=250, Percent_Identity=41.2, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_MYCA9 (B1MC85)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703510.1
- EnsemblBacteria:   EBMYCT00000006467
- GeneID:   5965285
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2777c
- GeneTree:   EBGT00050000016598
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- BioCyc:   MABS561007:MAB_2777C-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27268; Mature: 27137

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 100-100 ACT_SITE 172-172 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKPLIAGNWKMNLNHFEAIALVQKIAFSLPDKYFDKVDVTVIPPFTDIRSVQTLVDGD
CCCCCEEECCEEECCHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCHHHHHHHHHHCCC
KLRLTYGAQDLSVYDSGAYTGEVSGAFLAKLGVTYVVVGHSERRQYHGEDDALVAAKAAA
EEEEEECCCEEEEECCCCCCCCCCHHHHHHCCEEEEEEECCHHHHCCCCCCHHHHHHHHH
ALKHGLTPIVCIGEALDIREAGDHVQYNVNSLRGSLAGLSAEQVGKVVIAYEPVWAIGTG
HHHHCCCEEEEECCCCCHHHCCCCEEEEHHHHHHHHCCCCHHHHCCEEEEECCEEEECCC
RVASAADAQEVCAAIRAELAQIANADVAGSVRVLYGGSANAKNVGEIVAQEDVDGALVGG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEECC
ASLDGEQFAQMSAIAAGGPLL
CCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRKPLIAGNWKMNLNHFEAIALVQKIAFSLPDKYFDKVDVTVIPPFTDIRSVQTLVDGD
CCCCEEECCEEECCHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCHHHHHHHHHHCCC
KLRLTYGAQDLSVYDSGAYTGEVSGAFLAKLGVTYVVVGHSERRQYHGEDDALVAAKAAA
EEEEEECCCEEEEECCCCCCCCCCHHHHHHCCEEEEEEECCHHHHCCCCCCHHHHHHHHH
ALKHGLTPIVCIGEALDIREAGDHVQYNVNSLRGSLAGLSAEQVGKVVIAYEPVWAIGTG
HHHHCCCEEEEECCCCCHHHCCCCEEEEHHHHHHHHCCCCHHHHCCEEEEECCEEEECCC
RVASAADAQEVCAAIRAELAQIANADVAGSVRVLYGGSANAKNVGEIVAQEDVDGALVGG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEECC
ASLDGEQFAQMSAIAAGGPLL
CCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA