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The map label for this gene is hisH [H]

Identifier: 169629752

GI number: 169629752

Start: 2708988

End: 2709638

Strand: Reverse

Name: hisH [H]

Synonym: MAB_2667c

Alternate gene names: 169629752

Gene position: 2709638-2708988 (Counterclockwise)

Preceding gene: 169629753

Following gene: 169629751

Centisome position: 53.47

GC content: 62.67

Gene sequence:

>651_bases
GTGACCGGTGCTGGGCCGAAAGTTGTTGTCCTCGACTACGGCTCAGGCAATCTGAGGTCCGCACAGCGTGCACTGGAACG
GGTTGGCGCCGATGTTACGGTGACCGCCGACTCCAGTACGGCACTCAACGCTGACGGTCTGGTGGTCCCCGGTGTGGGAG
CCTTCGCGGCGTGTATGGAGGGACTGCGTGGTATCGACGGCGAACGCATCATCGATATCCGGCTCTCCGGGGGGCGCCCG
GTGCTGGGCATCTGTGTGGGAATGCAGATCCTGTTCAGTCACGGCATCGAATTCGGTGTCGATACGCAAGGGTGTGGGCA
GTGGCCCGGTGTGGTCAGCCGCCTCGATGCGCCCGTGATTCCGCATATGGGTTGGAACACCGTTGATGCGGCACCTGGTT
CGCAGCTCTTCGCTGGGCTCGACGCAGGCACCCGCTTTTATTTTGTGCATTCGTACGCGGTGCAGAAGTGGGAACTGGAA
ACGCCCGGCGAGTCGCCGATCGCGCCGCCGCTGGTGACCTGGGCGACCCACCACGTGCCGTTTGTCGCGGCGGTGGAGAA
TGGGCCACTTGCTGCCACACAGTTCCATCCGGAAAAGAGTGGTGACGCGGGGGCCGTGTTGTTGAGCAATTGGGTGAAGG
GAATCTCTTGA

Upstream 100 bases:

>100_bases
ACATCACCGAGGCGCAATACAAGGCGGTGGCCCGGGCGCTGCGCCAGGCCGTCGAGCCCGATCCCCGGGTCACCGGTGTG
CCGTCGACCAAGGGCAGCCT

Downstream 100 bases:

>100_bases
GTCTCGTTCTATTACCGGCGGTTGATGTCGCGGACGGTCAGGCTGTACGCCTGGTGCAGGGCAAGGCGGGCAGTGAAACC
ACGTACGGTTCGCCGCGTGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTGAGPKVVVLDYGSGNLRSAQRALERVGADVTVTADSSTALNADGLVVPGVGAFAACMEGLRGIDGERIIDIRLSGGRP
VLGICVGMQILFSHGIEFGVDTQGCGQWPGVVSRLDAPVIPHMGWNTVDAAPGSQLFAGLDAGTRFYFVHSYAVQKWELE
TPGESPIAPPLVTWATHHVPFVAAVENGPLAATQFHPEKSGDAGAVLLSNWVKGIS

Sequences:

>Translated_216_residues
MTGAGPKVVVLDYGSGNLRSAQRALERVGADVTVTADSSTALNADGLVVPGVGAFAACMEGLRGIDGERIIDIRLSGGRP
VLGICVGMQILFSHGIEFGVDTQGCGQWPGVVSRLDAPVIPHMGWNTVDAAPGSQLFAGLDAGTRFYFVHSYAVQKWELE
TPGESPIAPPLVTWATHHVPFVAAVENGPLAATQFHPEKSGDAGAVLLSNWVKGIS
>Mature_215_residues
TGAGPKVVVLDYGSGNLRSAQRALERVGADVTVTADSSTALNADGLVVPGVGAFAACMEGLRGIDGERIIDIRLSGGRPV
LGICVGMQILFSHGIEFGVDTQGCGQWPGVVSRLDAPVIPHMGWNTVDAAPGSQLFAGLDAGTRFYFVHSYAVQKWELET
PGESPIAPPLVTWATHHVPFVAAVENGPLAATQFHPEKSGDAGAVLLSNWVKGIS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=215, Percent_Identity=38.1395348837209, Blast_Score=130, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=221, Percent_Identity=36.1990950226244, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22486; Mature: 22355

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAGPKVVVLDYGSGNLRSAQRALERVGADVTVTADSSTALNADGLVVPGVGAFAACME
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEECCCCEEECCHHHHHHHHH
GLRGIDGERIIDIRLSGGRPVLGICVGMQILFSHGIEFGVDTQGCGQWPGVVSRLDAPVI
HHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCC
PHMGWNTVDAAPGSQLFAGLDAGTRFYFVHSYAVQKWELETPGESPIAPPLVTWATHHVP
CCCCCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCC
FVAAVENGPLAATQFHPEKSGDAGAVLLSNWVKGIS
EEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TGAGPKVVVLDYGSGNLRSAQRALERVGADVTVTADSSTALNADGLVVPGVGAFAACME
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEECCCCEEECCHHHHHHHHH
GLRGIDGERIIDIRLSGGRPVLGICVGMQILFSHGIEFGVDTQGCGQWPGVVSRLDAPVI
HHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCC
PHMGWNTVDAAPGSQLFAGLDAGTRFYFVHSYAVQKWELETPGESPIAPPLVTWATHHVP
CCCCCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHCCCC
FVAAVENGPLAATQFHPEKSGDAGAVLLSNWVKGIS
EEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA