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The map label for this gene is pyk [H]

Identifier: 169629724

GI number: 169629724

Start: 2680601

End: 2682019

Strand: Reverse

Name: pyk [H]

Synonym: MAB_2639c

Alternate gene names: 169629724

Gene position: 2682019-2680601 (Counterclockwise)

Preceding gene: 169629725

Following gene: 169629723

Centisome position: 52.93

GC content: 66.53

Gene sequence:

>1419_bases
GTGACGAGACGCGGAAAGATCGTTTGTACCCTCGGTCCTGCGACCGGTTCGGCCGAGCTTGTGCGCGCGCTCGTCGAGGC
CGGTATGGATGTCGCACGGTTGAACTTCAGCCACGGCGAGCACGCTGACCACGAGCAGAACTACCGCTGGGTGCGCAAGG
CATCCGATGAGTCCGGGCGGGCGGTCGGCGTGCTCGCCGATCTTCAGGGCCCCAAGATCAGGCTGGGCAGATTCGCTACC
GGGTCCGCCCACTGGGCCACCGGCGAACAGGTCCGGATCACCGTTGAGGACGTGGTAGGCACCCCGGACCGGGTTTCCAC
CACCTACAAGCAGTTGGCGGCGGATGCTGCCGTCGGCGACAGGCTCCTGGTCGACGATGGCAAGGTCGGCCTGACGGTCG
AAGCAATCGAGGGCGATGACGTCGTTTGCCGGGTGACCGAGGGGGGACCGGTCAGCAACAACAAGGGGTTGTCGCTGCCG
GGCATGAACGTCTCGGTGCCGGCCCTTTCCGAGAAGGACATCGCCGACTTGGAGTTCGCGCTGCGGCTCGGCGTCGATCT
CATCGCCCTGTCCTTCGTGCGCTCGCCCAGCGATGTGGAGCTGGTGCACGCCGTCATGGACCGTGTGGGACGCCGCGTGC
CGGTGATCGCCAAGCTGGAGAAGCCGGAGGCCGTCGCCAACCTCGAGGCGATCGTGTTGGCCTTCGACGCGATCATGGTC
GCCCGCGGGGATCTCGGTGTGGAGCTGCCGCTGGAAGATGTGCCCATTGTGCAGAAGCGGGCTATCCAGATCGCTCGGGA
AAACGCGCGCCCGGTCATCGTGGCCACCCAGATGCTCGAGTCGATGATCGAGAATTCGCGGCCCACCCGCGCCGAGGCCT
CCGATGTGGCCAACGCGGTCCTGGATGGTGCCGACGCCGTGATGCTGTCGGGGGAGACCTCGGTGGGCAAGTATCCGCTG
GAGGCGGTGCGGACCATGGCCCGCATCGTGTCCGCGGTGGAGAGCCATTCGGTGAGTGCGCCGCCGCTGACCCATGTTCC
GCGCACCAAACGTGGGGTCATCTCGTACGCGGCACGCGACATCGGGGAGCGCCTCAACGCCAAGGCCCTGGTCGCCTTCA
CGCAGTCCGGCGATACCGTCCGCCGGCTGGCCCGGCTGCATACTCCACTGCCGGTGCTGGCCTTCACCCCGCTGCCGGAG
GTGCGTAGCCAGCTGGCACTCACGTGGGGGACGGAAACCTTCATCGTCCCGGTCATGGAGACCACCGACGGCATGATTCA
ACAGGTGGATACGGCATTGCTGGAGATGGAGCGCTATCAGCGGGGAGATCTCGTGGTGATCGTCGCGGGCGCCCCACCCG
GGACAGTAGGCTCGACCAATCTGATCCACGTGCACCGGATCGGCGAGGACGACCACTAG

Upstream 100 bases:

>100_bases
ATTGGCGACCTGACAACGCAAACCGTTGAAGGTAACCGGTGAGTGAATCGTGCCCGCGCTGACTCGTGAGTAGGTGGCAC
CCCGACTATGCTCAAAAGTC

Downstream 100 bases:

>100_bases
GAGCTCAGCATCAGCAACGCACCGGACTTCGAACAGCTTCTCGCGCTGCTGGAACTGAAGAATCCCGAGACAGATGTGTT
CGTCGGTGGGCATCCGACTG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTRRGKIVCTLGPATGSAELVRALVEAGMDVARLNFSHGEHADHEQNYRWVRKASDESGRAVGVLADLQGPKIRLGRFAT
GSAHWATGEQVRITVEDVVGTPDRVSTTYKQLAADAAVGDRLLVDDGKVGLTVEAIEGDDVVCRVTEGGPVSNNKGLSLP
GMNVSVPALSEKDIADLEFALRLGVDLIALSFVRSPSDVELVHAVMDRVGRRVPVIAKLEKPEAVANLEAIVLAFDAIMV
ARGDLGVELPLEDVPIVQKRAIQIARENARPVIVATQMLESMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPL
EAVRTMARIVSAVESHSVSAPPLTHVPRTKRGVISYAARDIGERLNAKALVAFTQSGDTVRRLARLHTPLPVLAFTPLPE
VRSQLALTWGTETFIVPVMETTDGMIQQVDTALLEMERYQRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDH

Sequences:

>Translated_472_residues
MTRRGKIVCTLGPATGSAELVRALVEAGMDVARLNFSHGEHADHEQNYRWVRKASDESGRAVGVLADLQGPKIRLGRFAT
GSAHWATGEQVRITVEDVVGTPDRVSTTYKQLAADAAVGDRLLVDDGKVGLTVEAIEGDDVVCRVTEGGPVSNNKGLSLP
GMNVSVPALSEKDIADLEFALRLGVDLIALSFVRSPSDVELVHAVMDRVGRRVPVIAKLEKPEAVANLEAIVLAFDAIMV
ARGDLGVELPLEDVPIVQKRAIQIARENARPVIVATQMLESMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPL
EAVRTMARIVSAVESHSVSAPPLTHVPRTKRGVISYAARDIGERLNAKALVAFTQSGDTVRRLARLHTPLPVLAFTPLPE
VRSQLALTWGTETFIVPVMETTDGMIQQVDTALLEMERYQRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDH
>Mature_471_residues
TRRGKIVCTLGPATGSAELVRALVEAGMDVARLNFSHGEHADHEQNYRWVRKASDESGRAVGVLADLQGPKIRLGRFATG
SAHWATGEQVRITVEDVVGTPDRVSTTYKQLAADAAVGDRLLVDDGKVGLTVEAIEGDDVVCRVTEGGPVSNNKGLSLPG
MNVSVPALSEKDIADLEFALRLGVDLIALSFVRSPSDVELVHAVMDRVGRRVPVIAKLEKPEAVANLEAIVLAFDAIMVA
RGDLGVELPLEDVPIVQKRAIQIARENARPVIVATQMLESMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPLE
AVRTMARIVSAVESHSVSAPPLTHVPRTKRGVISYAARDIGERLNAKALVAFTQSGDTVRRLARLHTPLPVLAFTPLPEV
RSQLALTWGTETFIVPVMETTDGMIQQVDTALLEMERYQRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDH

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=37.1428571428571, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=37.1428571428571, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=37.3225152129817, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=37.3983739837398, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=37.3983739837398, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=37.9790940766551, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=37.9790940766551, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=36.1702127659575, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=36.1702127659575, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.5140186915888, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=40.8510638297872, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788160, Length=475, Percent_Identity=40.8421052631579, Blast_Score=288, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=36.2886597938144, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=37.8099173553719, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=37.8099173553719, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=37.8099173553719, Blast_Score=278, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=37.8099173553719, Blast_Score=277, Evalue=9e-75,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=33.8842975206612, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=33.0595482546201, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=35.9183673469388, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=35.9183673469388, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24648966, Length=320, Percent_Identity=41.875, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=28.9795918367347, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24646914, Length=283, Percent_Identity=35.3356890459364, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50575; Mature: 50443

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRGKIVCTLGPATGSAELVRALVEAGMDVARLNFSHGEHADHEQNYRWVRKASDESGR
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCC
AVGVLADLQGPKIRLGRFATGSAHWATGEQVRITVEDVVGTPDRVSTTYKQLAADAAVGD
EEEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCC
RLLVDDGKVGLTVEAIEGDDVVCRVTEGGPVSNNKGLSLPGMNVSVPALSEKDIADLEFA
EEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHH
LRLGVDLIALSFVRSPSDVELVHAVMDRVGRRVPVIAKLEKPEAVANLEAIVLAFDAIMV
HHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHE
ARGDLGVELPLEDVPIVQKRAIQIARENARPVIVATQMLESMIENSRPTRAEASDVANAV
ECCCCCEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADAVMLSGETSVGKYPLEAVRTMARIVSAVESHSVSAPPLTHVPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
IGERLNAKALVAFTQSGDTVRRLARLHTPLPVLAFTPLPEVRSQLALTWGTETFIVPVME
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHCEEEEECCCEEEEEEEC
TTDGMIQQVDTALLEMERYQRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDH
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TRRGKIVCTLGPATGSAELVRALVEAGMDVARLNFSHGEHADHEQNYRWVRKASDESGR
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCC
AVGVLADLQGPKIRLGRFATGSAHWATGEQVRITVEDVVGTPDRVSTTYKQLAADAAVGD
EEEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCC
RLLVDDGKVGLTVEAIEGDDVVCRVTEGGPVSNNKGLSLPGMNVSVPALSEKDIADLEFA
EEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHH
LRLGVDLIALSFVRSPSDVELVHAVMDRVGRRVPVIAKLEKPEAVANLEAIVLAFDAIMV
HHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHE
ARGDLGVELPLEDVPIVQKRAIQIARENARPVIVATQMLESMIENSRPTRAEASDVANAV
ECCCCCEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADAVMLSGETSVGKYPLEAVRTMARIVSAVESHSVSAPPLTHVPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
IGERLNAKALVAFTQSGDTVRRLARLHTPLPVLAFTPLPEVRSQLALTWGTETFIVPVME
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHCEEEEECCCEEEEEEEC
TTDGMIQQVDTALLEMERYQRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDH
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA