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The map label for this gene is braC [H]

Identifier: 169629711

GI number: 169629711

Start: 2666680

End: 2667864

Strand: Reverse

Name: braC [H]

Synonym: MAB_2626c

Alternate gene names: 169629711

Gene position: 2667864-2666680 (Counterclockwise)

Preceding gene: 169629712

Following gene: 169629710

Centisome position: 52.65

GC content: 61.94

Gene sequence:

>1185_bases
GTGCGTGCACGCGCGAGTGTGTTCATGCTGGGGGCGGTAGCCCTGGCGTTGGCGGGATGTCATGCCGGCGCGCAGACCGA
CAGCTCCCATCAGAGCAACCTGAAGATCGTGCCGCTGGTACAGATCGCCCAGGATGGCAGCGAGGTGCCGGCGTCCAACA
CGCAGTCGGCGGCCGATCCCGCGGGTGACGGAAAAGCTCAGTGCGCCAATGTTTCTCTGGCCACCGCGGGTGCGCTGAAC
GGCCCCGATTCGGCGCTCGGTACCAATATTCGCAACGGTGTCCAACTGGCTATCGACAAGCACAACGCCGCTAATCCCGG
ATGTCAGATCAAACTCCGTGAGTTCGATACCGAGGGGCTGCCGCAGAACGCGAGCAATATCGCCCCGCAGATCATCGGGG
ACCCGACGATCGTCGGGCTCATCGGACCCGCGTTTTCGGGCGAAACCAAGTCAACCGGGCAGATCTTCAGCGAGGCCGGC
CTGGTCTCCACGACCGGATCCGCCACCAACGTGACCCTCACCCAGCAGGGCTGGAAGACGTTCTTCCGCGGTCTGGCCAA
CGACGGGGTGCAGGGACCCGCTGTGGCCAACTACCTCAAGAAGACCCTTGGATACCAGAAGATCTGCGTGGTCGACGACA
GCACCGATTATGGATCCGGTCTAGCGAACGCGGTGCGCGGAACGCTCGGAAACGACTGCAATGTGTCGGTGAAAAAGGGC
GACAAGGACTTCTCCGCGGCGGTCGCACAGCTCAAGAACGCTGCACCGGACGCGGTGTATTTCGGTGGTTACTACGCCGA
AGCGGCGCCCCTGGTGCAGCAGCTGCGCAACGGAGGAGTCACCGCCACCTTCGTGTCCTCGGACGGCACCAAGGACGTCG
AGTTCGTCAAGCAGGCGGGCGACTCGGCCAAGGATGCATTGCTGTCCTGCCCGTGTGGGCCTGCTTCCAACAGTTTTGCC
AAGGATTACACGGCTAAGTTCAATCAGGAGCCCGGCACGTACAGCACGGAGGGTTATGACCTGGCCACGATCCTCGTCAA
GGGAATCGACGCCGGAAAGAGCACTCGGGCGGGGTTGCTTGATTGGGTCCGGAACTACCAGGGACAGGGCGTGGCGCGAG
AGTACAAGTGGAACGAAAACGGGGAGCTAACTTCCACACTTATCTGGGTGTATAAGGTCACGTAA

Upstream 100 bases:

>100_bases
CCGAGCTGAAACACGGGTAAGCACTGCGCGTCGCCCTCGGGTTGGCTATGTTTTCTGATCAGCGTTTCGACCAGACAGCC
CCAGCGATTCGGAGGTAACC

Downstream 100 bases:

>100_bases
CACATACGAGGGGACTAGGCTCAGCCGCAAATGATTTCGGGGTGGATGGCACAGACGGGTGTGCAGGCCAGCACAATTTC
ATTCGATCTGCACGGACTGT

Product: ABC transporter ligand-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MRARASVFMLGAVALALAGCHAGAQTDSSHQSNLKIVPLVQIAQDGSEVPASNTQSAADPAGDGKAQCANVSLATAGALN
GPDSALGTNIRNGVQLAIDKHNAANPGCQIKLREFDTEGLPQNASNIAPQIIGDPTIVGLIGPAFSGETKSTGQIFSEAG
LVSTTGSATNVTLTQQGWKTFFRGLANDGVQGPAVANYLKKTLGYQKICVVDDSTDYGSGLANAVRGTLGNDCNVSVKKG
DKDFSAAVAQLKNAAPDAVYFGGYYAEAAPLVQQLRNGGVTATFVSSDGTKDVEFVKQAGDSAKDALLSCPCGPASNSFA
KDYTAKFNQEPGTYSTEGYDLATILVKGIDAGKSTRAGLLDWVRNYQGQGVAREYKWNENGELTSTLIWVYKVT

Sequences:

>Translated_394_residues
MRARASVFMLGAVALALAGCHAGAQTDSSHQSNLKIVPLVQIAQDGSEVPASNTQSAADPAGDGKAQCANVSLATAGALN
GPDSALGTNIRNGVQLAIDKHNAANPGCQIKLREFDTEGLPQNASNIAPQIIGDPTIVGLIGPAFSGETKSTGQIFSEAG
LVSTTGSATNVTLTQQGWKTFFRGLANDGVQGPAVANYLKKTLGYQKICVVDDSTDYGSGLANAVRGTLGNDCNVSVKKG
DKDFSAAVAQLKNAAPDAVYFGGYYAEAAPLVQQLRNGGVTATFVSSDGTKDVEFVKQAGDSAKDALLSCPCGPASNSFA
KDYTAKFNQEPGTYSTEGYDLATILVKGIDAGKSTRAGLLDWVRNYQGQGVAREYKWNENGELTSTLIWVYKVT
>Mature_394_residues
MRARASVFMLGAVALALAGCHAGAQTDSSHQSNLKIVPLVQIAQDGSEVPASNTQSAADPAGDGKAQCANVSLATAGALN
GPDSALGTNIRNGVQLAIDKHNAANPGCQIKLREFDTEGLPQNASNIAPQIIGDPTIVGLIGPAFSGETKSTGQIFSEAG
LVSTTGSATNVTLTQQGWKTFFRGLANDGVQGPAVANYLKKTLGYQKICVVDDSTDYGSGLANAVRGTLGNDCNVSVKKG
DKDFSAAVAQLKNAAPDAVYFGGYYAEAAPLVQQLRNGGVTATFVSSDGTKDVEFVKQAGDSAKDALLSCPCGPASNSFA
KDYTAKFNQEPGTYSTEGYDLATILVKGIDAGKSTRAGLLDWVRNYQGQGVAREYKWNENGELTSTLIWVYKVT

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=332, Percent_Identity=29.8192771084337, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994941, Length=329, Percent_Identity=28.2674772036474, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40919; Mature: 40919

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARASVFMLGAVALALAGCHAGAQTDSSHQSNLKIVPLVQIAQDGSEVPASNTQSAADP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
AGDGKAQCANVSLATAGALNGPDSALGTNIRNGVQLAIDKHNAANPGCQIKLREFDTEGL
CCCCCCEEEEEEEEECCCCCCCCHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCC
PQNASNIAPQIIGDPTIVGLIGPAFSGETKSTGQIFSEAGLVSTTGSATNVTLTQQGWKT
CCCHHHCCCHHCCCCEEEEEECCCCCCCCCCHHHHHHHCCCEEECCCCEEEEEEHHHHHH
FFRGLANDGVQGPAVANYLKKTLGYQKICVVDDSTDYGSGLANAVRGTLGNDCNVSVKKG
HHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECC
DKDFSAAVAQLKNAAPDAVYFGGYYAEAAPLVQQLRNGGVTATFVSSDGTKDVEFVKQAG
CHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
DSAKDALLSCPCGPASNSFAKDYTAKFNQEPGTYSTEGYDLATILVKGIDAGKSTRAGLL
CCHHHHEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEHEEECCCCCCCCHHHHH
DWVRNYQGQGVAREYKWNENGELTSTLIWVYKVT
HHHHCCCCCCCEEECCCCCCCCEEEEEEEEEEEC
>Mature Secondary Structure
MRARASVFMLGAVALALAGCHAGAQTDSSHQSNLKIVPLVQIAQDGSEVPASNTQSAADP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
AGDGKAQCANVSLATAGALNGPDSALGTNIRNGVQLAIDKHNAANPGCQIKLREFDTEGL
CCCCCCEEEEEEEEECCCCCCCCHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCC
PQNASNIAPQIIGDPTIVGLIGPAFSGETKSTGQIFSEAGLVSTTGSATNVTLTQQGWKT
CCCHHHCCCHHCCCCEEEEEECCCCCCCCCCHHHHHHHCCCEEECCCCEEEEEEHHHHHH
FFRGLANDGVQGPAVANYLKKTLGYQKICVVDDSTDYGSGLANAVRGTLGNDCNVSVKKG
HHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECC
DKDFSAAVAQLKNAAPDAVYFGGYYAEAAPLVQQLRNGGVTATFVSSDGTKDVEFVKQAG
CHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
DSAKDALLSCPCGPASNSFAKDYTAKFNQEPGTYSTEGYDLATILVKGIDAGKSTRAGLL
CCHHHHEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEHEEECCCCCCCCHHHHH
DWVRNYQGQGVAREYKWNENGELTSTLIWVYKVT
HHHHCCCCCCCEEECCCCCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]