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The map label for this gene is sucB [H]

Identifier: 169629032

GI number: 169629032

Start: 1943280

End: 1944998

Strand: Reverse

Name: sucB [H]

Synonym: MAB_1945c

Alternate gene names: 169629032

Gene position: 1944998-1943280 (Counterclockwise)

Preceding gene: 169629035

Following gene: 169629031

Centisome position: 38.38

GC content: 70.56

Gene sequence:

>1719_bases
ATGGCCTTCTCCGTCCAGATGCCCGCCCTCGGTGAGAGCGTGACCGAAGGGACGGTGACGCGGTGGCTCAAGCAAGAAGG
CGACACGGTCGAAGTCGACGAGCCATTGCTCGAGGTGTCCACCGACAAGGTGGACACCGAGATCCCAGCTCCCACTTCGG
GTGTGTTGACCAAGATCGTCGCCCGTGAGGACGACACCGTCGAGATCGGCGGCGAGCTCGGTGTGATCAGCGAGGCCGGA
GAGGCGGCTCCCGCCGCCCCCGCCCCCGCCCCCGCCCCCGCAGCCGCACCGGCTCCGGAACCGACACCCGCACCGGCCCC
CGAAGCCACTGCTACGGCTGCTCCCGAACCCGCTGCCGAGCCGCCCGCCGCCGCTGCCCCGGCCGGCTCGGGCACCTCGG
TCAAGATGCCCGAGCTCGGTGAGTCCGTCACCGAAGGCACCGTGACCCGCTGGCTCAAGAAGGTCGGCGACGAGGTCGGC
GTGGACGAACCGCTCGTCGAGGTGTCCACCGACAAGGTCGACACCGAGATTCCGTCGCCGGTGGCCGGTGTGCTGCTCAG
CATCTCGGCCAACGAGGACGACACCGTGGCCGTGGGCGGCGAGCTTGCCGTCGTCGGAGCCGCCGACGCCGCGCCCGCGG
CCCCCGCAGCTCCTGCGGCGCCCGCTCCCGAGCCTGCCGCAGCTCCTGCCGCGGCAGCGGCACCGCCTGCTCCCCCAGCC
CCAGCCCCCGCACCGGCGGCCCCGGCTCCCGCATCGACGGCCCCGGCAGCGAACGGTGCCGCCGGCGACAACCCGTACGT
GACCCCGCTCGTGCGCAAGCTCGCCGCCGAAAACAACGTGGACCTTTCCGCGCTGTCCGGCAGCGGGGTGGGCGGACGCA
TCCGCAAGCAGGATGTGCTGGCCGCCGCGGAGGCCGCCAAGGCTCCGGCCGCGGCTCCCGCTCCCGCGGCTCCCGCCGCG
TCCGCGCCTGCGGCCGTAGCACCGTCCCTGGCGCACCTGCGCGGAACCACGCAGAAGGCCAACCGGATTCGTCAGATCAC
TGCCAAGAAGACGCGCGAGTCACTGCAGGAGACCGCCCAGCTGACCCAGACCCACGAGGTCGACGTCACCAAGATCGCGG
CCCTGCGCGCCCGGGCCAAGGCCGCGTTCGCCGAGCGCGAGGGTGTGAACCTGACGTTCCTGCCGTTCTTCGCCAAGGCC
GCGGTGGAGGCACTCAAGACGCACCCGAACGTCAACGCGAGCTACAACGAGGAATCCAAGGAGATCACCTACTTCGACGC
CGAGCACCTGGGCATCGCGGTCGACACCGACCAAGGCCTGCTCTCCCCGGTGATCCACAACGCCGGTGACCTGTCGCTGG
CCGGTCTGGCGCGCGCGATCGCCGATATCGCGGCACGGGCCCGTTCCGGGAACCTCAAGCCCGACGAGCTGGCCGGTGGC
ACCTTCACCATCACCAACATCGGCAGCCAGGGCGCCTTGTTCGACACGCCGATTCTGGTGCCGCCGCAGGCCGCCATGCT
CGGCACCGGCGCGATCGTGAAGCGTCCCAGGGTTATTCGTGATGACGCCGGTAACGAGTCGATCGGTATCCGGTCGGTGT
GCTACTTGCCGCTGACCTACGATCACCGCCTGATCGACGGAGCCGACGCGGGGCGGTTCCTGACGACCATCAAACACCGT
CTGGAAGAGGGGGCTTTCGAGGCGGACCTGGGTCTGTAG

Upstream 100 bases:

>100_bases
TCAGTGACAGGATTGACCCGGCTGACACGCAGCTGTGGTAAGCCATGAAGAGTCGTCTTTGTTAGTGCCGCTGTCCGAAC
CGTTTTCAGGAGTGGAAACC

Downstream 100 bases:

>100_bases
GCCTATGGGCGCTGAATCCCAGCACAAGCGGATCGTCATCGCCGGATCGTCGGGGGTGATCGGGTCGGCACTTGTCGCGT
CGCTGCGTTCCGCGAACCAC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MAFSVQMPALGESVTEGTVTRWLKQEGDTVEVDEPLLEVSTDKVDTEIPAPTSGVLTKIVAREDDTVEIGGELGVISEAG
EAAPAAPAPAPAPAAAPAPEPTPAPAPEATATAAPEPAAEPPAAAAPAGSGTSVKMPELGESVTEGTVTRWLKKVGDEVG
VDEPLVEVSTDKVDTEIPSPVAGVLLSISANEDDTVAVGGELAVVGAADAAPAAPAAPAAPAPEPAAAPAAAAAPPAPPA
PAPAPAAPAPASTAPAANGAAGDNPYVTPLVRKLAAENNVDLSALSGSGVGGRIRKQDVLAAAEAAKAPAAAPAPAAPAA
SAPAAVAPSLAHLRGTTQKANRIRQITAKKTRESLQETAQLTQTHEVDVTKIAALRARAKAAFAEREGVNLTFLPFFAKA
AVEALKTHPNVNASYNEESKEITYFDAEHLGIAVDTDQGLLSPVIHNAGDLSLAGLARAIADIAARARSGNLKPDELAGG
TFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIRDDAGNESIGIRSVCYLPLTYDHRLIDGADAGRFLTTIKHR
LEEGAFEADLGL

Sequences:

>Translated_572_residues
MAFSVQMPALGESVTEGTVTRWLKQEGDTVEVDEPLLEVSTDKVDTEIPAPTSGVLTKIVAREDDTVEIGGELGVISEAG
EAAPAAPAPAPAPAAAPAPEPTPAPAPEATATAAPEPAAEPPAAAAPAGSGTSVKMPELGESVTEGTVTRWLKKVGDEVG
VDEPLVEVSTDKVDTEIPSPVAGVLLSISANEDDTVAVGGELAVVGAADAAPAAPAAPAAPAPEPAAAPAAAAAPPAPPA
PAPAPAAPAPASTAPAANGAAGDNPYVTPLVRKLAAENNVDLSALSGSGVGGRIRKQDVLAAAEAAKAPAAAPAPAAPAA
SAPAAVAPSLAHLRGTTQKANRIRQITAKKTRESLQETAQLTQTHEVDVTKIAALRARAKAAFAEREGVNLTFLPFFAKA
AVEALKTHPNVNASYNEESKEITYFDAEHLGIAVDTDQGLLSPVIHNAGDLSLAGLARAIADIAARARSGNLKPDELAGG
TFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIRDDAGNESIGIRSVCYLPLTYDHRLIDGADAGRFLTTIKHR
LEEGAFEADLGL
>Mature_571_residues
AFSVQMPALGESVTEGTVTRWLKQEGDTVEVDEPLLEVSTDKVDTEIPAPTSGVLTKIVAREDDTVEIGGELGVISEAGE
AAPAAPAPAPAPAAAPAPEPTPAPAPEATATAAPEPAAEPPAAAAPAGSGTSVKMPELGESVTEGTVTRWLKKVGDEVGV
DEPLVEVSTDKVDTEIPSPVAGVLLSISANEDDTVAVGGELAVVGAADAAPAAPAAPAAPAPEPAAAPAAAAAPPAPPAP
APAPAAPAPASTAPAANGAAGDNPYVTPLVRKLAAENNVDLSALSGSGVGGRIRKQDVLAAAEAAKAPAAAPAPAAPAAS
APAAVAPSLAHLRGTTQKANRIRQITAKKTRESLQETAQLTQTHEVDVTKIAALRARAKAAFAEREGVNLTFLPFFAKAA
VEALKTHPNVNASYNEESKEITYFDAEHLGIAVDTDQGLLSPVIHNAGDLSLAGLARAIADIAARARSGNLKPDELAGGT
FTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIRDDAGNESIGIRSVCYLPLTYDHRLIDGADAGRFLTTIKHRL
EEGAFEADLGL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=464, Percent_Identity=29.7413793103448, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=40.2654867256637, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI31711992, Length=577, Percent_Identity=28.42287694974, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=25.9179265658747, Blast_Score=136, Evalue=7e-32,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=25.8134490238612, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI260898739, Length=155, Percent_Identity=33.5483870967742, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=36.8663594470046, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1786305, Length=566, Percent_Identity=30.9187279151943, Blast_Score=175, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=439, Percent_Identity=32.3462414578588, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=443, Percent_Identity=27.5395033860045, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=31.1659192825112, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=324, Percent_Identity=29.0123456790123, Blast_Score=96, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=455, Percent_Identity=32.5274725274725, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=26.5795206971678, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18859875, Length=447, Percent_Identity=29.3064876957494, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=37.3333333333333, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI20129315, Length=447, Percent_Identity=29.7539149888143, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24582497, Length=439, Percent_Identity=31.6628701594533, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 57660; Mature: 57529

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSVQMPALGESVTEGTVTRWLKQEGDTVEVDEPLLEVSTDKVDTEIPAPTSGVLTKIV
CCEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHH
AREDDTVEIGGELGVISEAGEAAPAAPAPAPAPAAAPAPEPTPAPAPEATATAAPEPAAE
HCCCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPAAAAPAGSGTSVKMPELGESVTEGTVTRWLKKVGDEVGVDEPLVEVSTDKVDTEIPSP
CCCCCCCCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
VAGVLLSISANEDDTVAVGGELAVVGAADAAPAAPAAPAAPAPEPAAAPAAAAAPPAPPA
HHEEEEEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPAPAAPAPASTAPAANGAAGDNPYVTPLVRKLAAENNVDLSALSGSGVGGRIRKQDVL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHH
AAAEAAKAPAAAPAPAAPAASAPAAVAPSLAHLRGTTQKANRIRQITAKKTRESLQETAQ
HHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTQTHEVDVTKIAALRARAKAAFAEREGVNLTFLPFFAKAAVEALKTHPNVNASYNEESK
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCC
EITYFDAEHLGIAVDTDQGLLSPVIHNAGDLSLAGLARAIADIAARARSGNLKPDELAGG
EEEEEEHHHCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
TFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIRDDAGNESIGIRSVCYLPLTY
EEEEEECCCCCCEECCCEECCCCHHHHCCCHHHCCCEEEECCCCCCCCCCEEEEEEEECC
DHRLIDGADAGRFLTTIKHRLEEGAFEADLGL
CCEEECCCCCHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
AFSVQMPALGESVTEGTVTRWLKQEGDTVEVDEPLLEVSTDKVDTEIPAPTSGVLTKIV
CEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHH
AREDDTVEIGGELGVISEAGEAAPAAPAPAPAPAAAPAPEPTPAPAPEATATAAPEPAAE
HCCCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPAAAAPAGSGTSVKMPELGESVTEGTVTRWLKKVGDEVGVDEPLVEVSTDKVDTEIPSP
CCCCCCCCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
VAGVLLSISANEDDTVAVGGELAVVGAADAAPAAPAAPAAPAPEPAAAPAAAAAPPAPPA
HHEEEEEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPAPAAPAPASTAPAANGAAGDNPYVTPLVRKLAAENNVDLSALSGSGVGGRIRKQDVL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHH
AAAEAAKAPAAAPAPAAPAASAPAAVAPSLAHLRGTTQKANRIRQITAKKTRESLQETAQ
HHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTQTHEVDVTKIAALRARAKAAFAEREGVNLTFLPFFAKAAVEALKTHPNVNASYNEESK
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCC
EITYFDAEHLGIAVDTDQGLLSPVIHNAGDLSLAGLARAIADIAARARSGNLKPDELAGG
EEEEEEHHHCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
TFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIRDDAGNESIGIRSVCYLPLTY
EEEEEECCCCCCEECCCEECCCCHHHHCCCHHHCCCEEEECCCCCCCCCCEEEEEEEECC
DHRLIDGADAGRFLTTIKHRLEEGAFEADLGL
CCEEECCCCCHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]