BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is yqhE [C]

Identifier: 169628619

GI number: 169628619

Start: 1548465

End: 1549280

Strand: Reverse

Name: yqhE [C]

Synonym: MAB_1528c

Alternate gene names: 169628619

Gene position: 1549280-1548465 (Counterclockwise)

Preceding gene: 169628620

Following gene: 169628616

Centisome position: 30.57

GC content: 65.07

Gene sequence:

>816_bases
GTGACGCTCAATTCCGGCACCACGATCCCGCAGCTCGGCTACGGCGTCTGGCAGGTTCCCGACGACCAGGCGCGTGCGGC
AGTGTCGGCAGCCCTGCAGGTCGGATATCGCAGCATCGACACCGCCAAGATCTACGACAACGAGGCGGGCACTGGCGCCG
CCATCGCGGAATCCGGCATTCCGCGCGGCGATGTCTTCCTGACCACCAAGCTGTGGAACTCCGACCAGGGATACGACAAC
GCGCTACGCGCCTTCGACGCCTCCCTGGAGCGGCTGGGCACCGATTACGTGGACCTGTACCTCATCCACTGGCCGGTCCC
CGAACTCGATGAGTACGTGGCGAGCTTCAAGGCCCTACAGCGGATCCAGGCCGATGGGCGCGCCAAGGCCATCGGCGTCA
GCAACTTCACCGTCGACAACCTCAAGCGGCTTATCGATGAGACCGGAGAAGTACCCGCCCTCAACCAGATCGAGCTACAC
CCGCGGTTCACCCAGCCCGAACTGCGCGCGTTCCACGCCGAATACGGCATTGCCACCGAGGCGTGGTCGCCGCTCGGCCA
GGGCACGATTCTGGAGGATGCGACGATCGGCGCCATCGCCCAGGCACACGACGTGAGTGCGGCACAGGTGATCCTGCGCT
GGCATCTGCAGCTCGGTAACGTCGTCATCCCCAAGTCGGTGACGCCGGCCCGCATTGCGGCCAACTTCGACGTATTCGGT
TTCGAGCTCAGCACCGACGAGGTGGAGCGCATCACCGCGCTGGACGCGTCCGACGGCCGTATCGGGCCGGATCCGACAAC
GTTCAACTTGCACTAG

Upstream 100 bases:

>100_bases
ATGGCCGTACATCGTGTTTCATTCTGCTGCGGCTGCCCGGGAATACTCGACGGCGCCTCGTGTTGGGTTGACCGTGACAG
TTCCCCAGATAGTCCCCAAT

Downstream 100 bases:

>100_bases
CGCGGATCACCGCACGATGTAGTGGCTCTCCTCGGGTGAGGCCAGCATCTGCTCCATCGTCTTGCGGTCAAAGGGCCACA
GGTCAACGGATCCGTCCTCG

Product: oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTLNSGTTIPQLGYGVWQVPDDQARAAVSAALQVGYRSIDTAKIYDNEAGTGAAIAESGIPRGDVFLTTKLWNSDQGYDN
ALRAFDASLERLGTDYVDLYLIHWPVPELDEYVASFKALQRIQADGRAKAIGVSNFTVDNLKRLIDETGEVPALNQIELH
PRFTQPELRAFHAEYGIATEAWSPLGQGTILEDATIGAIAQAHDVSAAQVILRWHLQLGNVVIPKSVTPARIAANFDVFG
FELSTDEVERITALDASDGRIGPDPTTFNLH

Sequences:

>Translated_271_residues
MTLNSGTTIPQLGYGVWQVPDDQARAAVSAALQVGYRSIDTAKIYDNEAGTGAAIAESGIPRGDVFLTTKLWNSDQGYDN
ALRAFDASLERLGTDYVDLYLIHWPVPELDEYVASFKALQRIQADGRAKAIGVSNFTVDNLKRLIDETGEVPALNQIELH
PRFTQPELRAFHAEYGIATEAWSPLGQGTILEDATIGAIAQAHDVSAAQVILRWHLQLGNVVIPKSVTPARIAANFDVFG
FELSTDEVERITALDASDGRIGPDPTTFNLH
>Mature_270_residues
TLNSGTTIPQLGYGVWQVPDDQARAAVSAALQVGYRSIDTAKIYDNEAGTGAAIAESGIPRGDVFLTTKLWNSDQGYDNA
LRAFDASLERLGTDYVDLYLIHWPVPELDEYVASFKALQRIQADGRAKAIGVSNFTVDNLKRLIDETGEVPALNQIELHP
RFTQPELRAFHAEYGIATEAWSPLGQGTILEDATIGAIAQAHDVSAAQVILRWHLQLGNVVIPKSVTPARIAANFDVFGF
ELSTDEVERITALDASDGRIGPDPTTFNLH

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=289, Percent_Identity=35.2941176470588, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI24497577, Length=294, Percent_Identity=34.3537414965986, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI5174391, Length=294, Percent_Identity=34.3537414965986, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=36.7272727272727, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI310109920, Length=293, Percent_Identity=34.4709897610922, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI5453543, Length=293, Percent_Identity=34.4709897610922, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI4502049, Length=282, Percent_Identity=38.2978723404255, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI45446745, Length=293, Percent_Identity=34.4709897610922, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI4503285, Length=293, Percent_Identity=34.4709897610922, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=33.1081081081081, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=32.4232081911263, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI93277124, Length=308, Percent_Identity=32.4675324675325, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI291291012, Length=267, Percent_Identity=34.0823970037453, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=32.3024054982818, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=33.2129963898917, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI300116271, Length=276, Percent_Identity=31.5217391304348, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI310109926, Length=113, Percent_Identity=40.7079646017699, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI207028673, Length=113, Percent_Identity=40.7079646017699, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI310109924, Length=150, Percent_Identity=34.6666666666667, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI87082198, Length=270, Percent_Identity=48.5185185185185, Blast_Score=270, Evalue=8e-74,
Organism=Escherichia coli, GI1786400, Length=258, Percent_Identity=39.5348837209302, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1788081, Length=275, Percent_Identity=33.4545454545455, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87081735, Length=201, Percent_Identity=28.3582089552239, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788070, Length=217, Percent_Identity=27.1889400921659, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=35.8620689655172, Blast_Score=182, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71998625, Length=265, Percent_Identity=34.7169811320755, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537075, Length=298, Percent_Identity=35.5704697986577, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17561298, Length=269, Percent_Identity=36.4312267657993, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=37.7289377289377, Blast_Score=177, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17564128, Length=293, Percent_Identity=32.4232081911263, Blast_Score=160, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=32.8301886792453, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17537079, Length=299, Percent_Identity=30.7692307692308, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17561300, Length=272, Percent_Identity=32.7205882352941, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17566692, Length=289, Percent_Identity=32.5259515570934, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17550248, Length=283, Percent_Identity=31.8021201413428, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=31.2727272727273, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320576, Length=289, Percent_Identity=41.1764705882353, Blast_Score=219, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324694, Length=289, Percent_Identity=41.1764705882353, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6319625, Length=290, Percent_Identity=34.1379310344828, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6322556, Length=265, Percent_Identity=32.0754716981132, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321896, Length=305, Percent_Identity=31.1475409836066, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320079, Length=284, Percent_Identity=33.4507042253521, Blast_Score=135, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=35.0340136054422, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24657054, Length=275, Percent_Identity=38.5454545454545, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24663317, Length=299, Percent_Identity=33.1103678929766, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24644950, Length=295, Percent_Identity=34.2372881355932, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI20129731, Length=276, Percent_Identity=37.3188405797101, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21356425, Length=275, Percent_Identity=35.2727272727273, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553081, Length=285, Percent_Identity=32.6315789473684, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=32.1766561514196, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24662781, Length=295, Percent_Identity=36.6101694915254, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24662785, Length=290, Percent_Identity=36.8965517241379, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI281366140, Length=253, Percent_Identity=32.0158102766798, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 29490; Mature: 29359

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNSGTTIPQLGYGVWQVPDDQARAAVSAALQVGYRSIDTAKIYDNEAGTGAAIAESGI
CCCCCCCCCHHHCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCC
PRGDVFLTTKLWNSDQGYDNALRAFDASLERLGTDYVDLYLIHWPVPELDEYVASFKALQ
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHH
RIQADGRAKAIGVSNFTVDNLKRLIDETGEVPALNQIELHPRFTQPELRAFHAEYGIATE
HHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCHH
AWSPLGQGTILEDATIGAIAQAHDVSAAQVILRWHLQLGNVVIPKSVTPARIAANFDVFG
HCCCCCCCCEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCCCCHHEEECCEEEE
FELSTDEVERITALDASDGRIGPDPTTFNLH
EEECHHHHHHHEEECCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TLNSGTTIPQLGYGVWQVPDDQARAAVSAALQVGYRSIDTAKIYDNEAGTGAAIAESGI
CCCCCCCCHHHCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCC
PRGDVFLTTKLWNSDQGYDNALRAFDASLERLGTDYVDLYLIHWPVPELDEYVASFKALQ
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHH
RIQADGRAKAIGVSNFTVDNLKRLIDETGEVPALNQIELHPRFTQPELRAFHAEYGIATE
HHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCHH
AWSPLGQGTILEDATIGAIAQAHDVSAAQVILRWHLQLGNVVIPKSVTPARIAANFDVFG
HCCCCCCCCEECCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCCCCHHEEECCEEEE
FELSTDEVERITALDASDGRIGPDPTTFNLH
EEECHHHHHHHEEECCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA