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The map label for this gene is pcpB [H]

Identifier: 169628587

GI number: 169628587

Start: 1509769

End: 1511196

Strand: Reverse

Name: pcpB [H]

Synonym: MAB_1496c

Alternate gene names: 169628587

Gene position: 1511196-1509769 (Counterclockwise)

Preceding gene: 169628588

Following gene: 169628584

Centisome position: 29.82

GC content: 62.32

Gene sequence:

>1428_bases
ATGACAGTGGTGATCGCCGGGGCCGGCCCCACGGGCCTGACGCTGGCGTGCGAATTGACGCGGCGCGGCATCGCATGCCG
GGTGCTGGACAAGGCTCCCGATCTGTTCCCCGGTTCTCGCGGCAAGGGCCTGTCCCCGCGCACCCAGGAGGTGTTCGACG
ATCTCGGTATCGCGCCTGCGATCAACAGCGGGGGCATGGCCATGCCGCCCTTCCGCATCTACGCGGGCCATGAGGTCGTC
GCCGAGCGCAGCCTTGTGGAGATGCTCGGGACCGACATCCCCTCGGGCCCAGGCATCCCATACCCAGGTTTTTGGTTGGT
GCCGCAATGGCGTACCGACGAGATCCTGTTGGACAGGCTGCGTCAGTTCGGAGGCGATGTGGAATTCAACTGTGAGGTAG
TCGGTTTCACGCAGAGATCGGATGCGGTCAGCGTGATGGTGTCCCAGGGTGGCGCGCCCGAATTGCTCCACGCCTCATAC
CTCGTCGGTGCCGACGGTGGGCGCAGCACAGTACGCAAGATGCTCGGCGTGGGGTTTGCCGGGGAGACGTTCGAGAGGGA
ACGAACGCTGATCGGCGATGTGCGGGCCGATGGCTTGGAGGGATCCTTCTGCCATGTGCTGACACGGGATGGTCAAGTGT
CGGAACGCTTTTCGCTCTGGAATCTACCGGGCAGTGAGCACTATCAATTTGTCGCGAACATGGCTACCGAGGATGTTCCC
GCGCTGACACTGGACGCGGTTCAGAAACTGGTCGTCGATCGCTCCGGCCGGGACGACATTGTGTTGCGGGATCTGCGATG
GATTTCCCTCTACCGCGTGAATGCGCGCATGGTGGACAGATTTCGGGTGGGACGAGTGATCCTGGCCGGTGATGCGGCGC
ACGTGCATTCCTCGGCGGGCGGCCAGGGCCTGAACACGAGTGTGCAAGACGCCTACAACCTGGGATGGAAGCTGGCAGCC
GTCATCTACGGCGCCCCCGAGAAATTGCTAGACACCTATGAGGAAGAACGGATGCCGGTGGCCGCATCCGTGCTGGGACT
GAGCACCGACCTGCACCATCGCAACTTCGCGCCTGCGAAAGGACCTGCGCCTCAGTTACATCAGATGGACATCACATATC
GTGGATGCTCGTTGGCCGTCGACGATCGCGTGTTTCAAGGAAATCTCCGAGCCGGTGACCGGGCACCTGACGCCTTGCTA
GACAACGGGGTACGCCTGTTTGATGTGCTACGTGGCACGCATTTCACGCTGTTGACGTTCGGCGCCCAGGCTCCCGTCAT
CGCCGACGTGTGCATTCAACAGATGACGCCATCGCCGGACTACGACGTTACGGCAACGACACTGGTGCTGGTACGCCCGG
ACGGGTACATCGGTGTCATGACCGAATCCGGGCGCACAGTCCTCGAGTATCTCGCCCGTGTTGTCTAG

Upstream 100 bases:

>100_bases
TGCTTACCCATCGCCTGACCCTGGGCGGCCCCCTTGACGACGCGTTCATCGGCCGGCTCGTCGATGACGTCTTGCTCGTC
CTTATCCGACCCGGGGCCCG

Downstream 100 bases:

>100_bases
ATGGCGGCGGTCAGCGTCTCCAAACGCGCGGGGACATGCTTGTGCCATGGCGTTCCGGCGTACTCGTCACGCCAAGCCGA
CGAGGTGAGAGCATTCGGCG

Product: putative FAD-binding monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTVVIAGAGPTGLTLACELTRRGIACRVLDKAPDLFPGSRGKGLSPRTQEVFDDLGIAPAINSGGMAMPPFRIYAGHEVV
AERSLVEMLGTDIPSGPGIPYPGFWLVPQWRTDEILLDRLRQFGGDVEFNCEVVGFTQRSDAVSVMVSQGGAPELLHASY
LVGADGGRSTVRKMLGVGFAGETFERERTLIGDVRADGLEGSFCHVLTRDGQVSERFSLWNLPGSEHYQFVANMATEDVP
ALTLDAVQKLVVDRSGRDDIVLRDLRWISLYRVNARMVDRFRVGRVILAGDAAHVHSSAGGQGLNTSVQDAYNLGWKLAA
VIYGAPEKLLDTYEEERMPVAASVLGLSTDLHHRNFAPAKGPAPQLHQMDITYRGCSLAVDDRVFQGNLRAGDRAPDALL
DNGVRLFDVLRGTHFTLLTFGAQAPVIADVCIQQMTPSPDYDVTATTLVLVRPDGYIGVMTESGRTVLEYLARVV

Sequences:

>Translated_475_residues
MTVVIAGAGPTGLTLACELTRRGIACRVLDKAPDLFPGSRGKGLSPRTQEVFDDLGIAPAINSGGMAMPPFRIYAGHEVV
AERSLVEMLGTDIPSGPGIPYPGFWLVPQWRTDEILLDRLRQFGGDVEFNCEVVGFTQRSDAVSVMVSQGGAPELLHASY
LVGADGGRSTVRKMLGVGFAGETFERERTLIGDVRADGLEGSFCHVLTRDGQVSERFSLWNLPGSEHYQFVANMATEDVP
ALTLDAVQKLVVDRSGRDDIVLRDLRWISLYRVNARMVDRFRVGRVILAGDAAHVHSSAGGQGLNTSVQDAYNLGWKLAA
VIYGAPEKLLDTYEEERMPVAASVLGLSTDLHHRNFAPAKGPAPQLHQMDITYRGCSLAVDDRVFQGNLRAGDRAPDALL
DNGVRLFDVLRGTHFTLLTFGAQAPVIADVCIQQMTPSPDYDVTATTLVLVRPDGYIGVMTESGRTVLEYLARVV
>Mature_474_residues
TVVIAGAGPTGLTLACELTRRGIACRVLDKAPDLFPGSRGKGLSPRTQEVFDDLGIAPAINSGGMAMPPFRIYAGHEVVA
ERSLVEMLGTDIPSGPGIPYPGFWLVPQWRTDEILLDRLRQFGGDVEFNCEVVGFTQRSDAVSVMVSQGGAPELLHASYL
VGADGGRSTVRKMLGVGFAGETFERERTLIGDVRADGLEGSFCHVLTRDGQVSERFSLWNLPGSEHYQFVANMATEDVPA
LTLDAVQKLVVDRSGRDDIVLRDLRWISLYRVNARMVDRFRVGRVILAGDAAHVHSSAGGQGLNTSVQDAYNLGWKLAAV
IYGAPEKLLDTYEEERMPVAASVLGLSTDLHHRNFAPAKGPAPQLHQMDITYRGCSLAVDDRVFQGNLRAGDRAPDALLD
NGVRLFDVLRGTHFTLLTFGAQAPVIADVCIQQMTPSPDYDVTATTLVLVRPDGYIGVMTESGRTVLEYLARVV

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=367, Percent_Identity=29.1553133514986, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 51510; Mature: 51379

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVIAGAGPTGLTLACELTRRGIACRVLDKAPDLFPGSRGKGLSPRTQEVFDDLGIAPA
CEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
INSGGMAMPPFRIYAGHEVVAERSLVEMLGTDIPSGPGIPYPGFWLVPQWRTDEILLDRL
CCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHH
RQFGGDVEFNCEVVGFTQRSDAVSVMVSQGGAPELLHASYLVGADGGRSTVRKMLGVGFA
HHHCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHCCCCC
GETFERERTLIGDVRADGLEGSFCHVLTRDGQVSERFSLWNLPGSEHYQFVANMATEDVP
CCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCC
ALTLDAVQKLVVDRSGRDDIVLRDLRWISLYRVNARMVDRFRVGRVILAGDAAHVHSSAG
CHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECHHHHHHHHHCEEEEECCHHHHCCCCC
GQGLNTSVQDAYNLGWKLAAVIYGAPEKLLDTYEEERMPVAASVLGLSTDLHHRNFAPAK
CCCCCCCHHHHHHCCEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
GPAPQLHQMDITYRGCSLAVDDRVFQGNLRAGDRAPDALLDNGVRLFDVLRGTHFTLLTF
CCCCCCEEEEEEECCCEEEECCEEEECCCCCCCCCCHHHHHCCHHHHHHHCCCEEEEEEE
GAQAPVIADVCIQQMTPSPDYDVTATTLVLVRPDGYIGVMTESGRTVLEYLARVV
CCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHC
>Mature Secondary Structure 
TVVIAGAGPTGLTLACELTRRGIACRVLDKAPDLFPGSRGKGLSPRTQEVFDDLGIAPA
EEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
INSGGMAMPPFRIYAGHEVVAERSLVEMLGTDIPSGPGIPYPGFWLVPQWRTDEILLDRL
CCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHH
RQFGGDVEFNCEVVGFTQRSDAVSVMVSQGGAPELLHASYLVGADGGRSTVRKMLGVGFA
HHHCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHCCCCC
GETFERERTLIGDVRADGLEGSFCHVLTRDGQVSERFSLWNLPGSEHYQFVANMATEDVP
CCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCC
ALTLDAVQKLVVDRSGRDDIVLRDLRWISLYRVNARMVDRFRVGRVILAGDAAHVHSSAG
CHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECHHHHHHHHHCEEEEECCHHHHCCCCC
GQGLNTSVQDAYNLGWKLAAVIYGAPEKLLDTYEEERMPVAASVLGLSTDLHHRNFAPAK
CCCCCCCHHHHHHCCEEEEEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
GPAPQLHQMDITYRGCSLAVDDRVFQGNLRAGDRAPDALLDNGVRLFDVLRGTHFTLLTF
CCCCCCEEEEEEECCCEEEECCEEEECCCCCCCCCCHHHHHCCHHHHHHHCCCEEEEEEE
GAQAPVIADVCIQQMTPSPDYDVTATTLVLVRPDGYIGVMTESGRTVLEYLARVV
CCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]