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The map label for this gene is folP2 [H]

Identifier: 169628438

GI number: 169628438

Start: 1346566

End: 1347474

Strand: Direct

Name: folP2 [H]

Synonym: MAB_1345

Alternate gene names: 169628438

Gene position: 1346566-1347474 (Clockwise)

Preceding gene: 169628436

Following gene: 169628439

Centisome position: 26.57

GC content: 66.78

Gene sequence:

>909_bases
ATGGCGAGGCGGGCTGCCCTGCACAATGAGGACGTGCGTGAGACGTTTTGTGGACGACCCGTCCGCACCGATCGTGCGCT
CATCATGGCGATCGTGAACCGCACACCGGACTCGTTCTATGACCGCGGCGCCACCTTTACCGACGAGGCGGCGCAGGCGG
CGGTGCATCAAGCGGTGGCCGATGGTGCGGATGTCGTCGACATCGGTGGCGTCAAGGCGGGCCCGGGGGAGAACGTCGAC
GCCGACGCCGAAATCGCGCGGGTGGTGCCCTTCATCGAATGGGTGCGCGGCGCCCACCCCGACTTGCTGATCAGCGTGGA
TACCTGGCGCGCCGAGGTTGCGGCGAAGGCCTGTGCGGCAGGAGCCGATCTGATCAACGACACCTGGGCGGGTGCCGATC
CCGAGCTACCGGCCGTGGCGGCCGCGTACGGCGCCGGATTGGTTTGCTCCCACACCGGTGGCGCGGTACCGCGTACCCGG
CCGTATCGCGTCCATTACGGAACCGAGGTCGACGGCGTCGTGCGGGCGGTGCTCGACGAAGTGACCGCGTCCGCCGAGCG
GGCGCTCGCCCTGGGGGTGTCGAAGGACGCGATTCTGATCGATCCGACGCATGACTTCGGCAAAAACACCTTCCACGGGC
TCGCTTTGTTGCGCCACGTAAACGATCTCGTTAATACCGGGTGGCCCGTGTTGATGGCGTTGAGCAACAAGGATTTCATC
GGGGAGACTCTTGGTGTGGGACTGACGGAACGACTCGAGGGCACCCTCGCCGCCACCGCACTGGCGGCTGCTGCGGGTGC
TCGCATGTTCCGGGTTCATGAGGTCGGGCCCACACGCCGCACGCTGGAAATGGTGGGCTCCATCGTCGGGACCCGCCCAC
CGGCGCGAACAGTGAGGGGGTTGGCATGA

Upstream 100 bases:

>100_bases
TGTGCCCATACTGCCGAGGCCCGCAAGTCATTTCGCGTGCCGAGAGTGAAGCCGTTGCGGGAAATCGGTCCAAATCCCGC
AGCAGCTTCACACTCGGCGC

Downstream 100 bases:

>100_bases
CCTCAACCGTTGGTATCGACTCAAGCTGGATGTCGCCGGCGCCACAGCTCCCGGGCTGGCTGGAGACCCACAGCTGGAAC
CGGCCCGAATGGACCGTCGA

Product: dihydropteroate synthase

Products: diphosphate; dihydropteroate

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2 [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MARRAALHNEDVRETFCGRPVRTDRALIMAIVNRTPDSFYDRGATFTDEAAQAAVHQAVADGADVVDIGGVKAGPGENVD
ADAEIARVVPFIEWVRGAHPDLLISVDTWRAEVAAKACAAGADLINDTWAGADPELPAVAAAYGAGLVCSHTGGAVPRTR
PYRVHYGTEVDGVVRAVLDEVTASAERALALGVSKDAILIDPTHDFGKNTFHGLALLRHVNDLVNTGWPVLMALSNKDFI
GETLGVGLTERLEGTLAATALAAAAGARMFRVHEVGPTRRTLEMVGSIVGTRPPARTVRGLA

Sequences:

>Translated_302_residues
MARRAALHNEDVRETFCGRPVRTDRALIMAIVNRTPDSFYDRGATFTDEAAQAAVHQAVADGADVVDIGGVKAGPGENVD
ADAEIARVVPFIEWVRGAHPDLLISVDTWRAEVAAKACAAGADLINDTWAGADPELPAVAAAYGAGLVCSHTGGAVPRTR
PYRVHYGTEVDGVVRAVLDEVTASAERALALGVSKDAILIDPTHDFGKNTFHGLALLRHVNDLVNTGWPVLMALSNKDFI
GETLGVGLTERLEGTLAATALAAAAGARMFRVHEVGPTRRTLEMVGSIVGTRPPARTVRGLA
>Mature_301_residues
ARRAALHNEDVRETFCGRPVRTDRALIMAIVNRTPDSFYDRGATFTDEAAQAAVHQAVADGADVVDIGGVKAGPGENVDA
DAEIARVVPFIEWVRGAHPDLLISVDTWRAEVAAKACAAGADLINDTWAGADPELPAVAAAYGAGLVCSHTGGAVPRTRP
YRVHYGTEVDGVVRAVLDEVTASAERALALGVSKDAILIDPTHDFGKNTFHGLALLRHVNDLVNTGWPVLMALSNKDFIG
ETLGVGLTERLEGTLAATALAAAAGARMFRVHEVGPTRRTLEMVGSIVGTRPPARTVRGLA

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism [H]

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082224, Length=270, Percent_Identity=32.5925925925926, Blast_Score=134, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6324073, Length=320, Percent_Identity=28.4375, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489 [H]

Pfam domain/function: PF00809 Pterin_bind [H]

EC number: 2.5.1.15

Molecular weight: Translated: 31810; Mature: 31679

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50972 PTERIN_BINDING ; PS00792 DHPS_1 ; PS00793 DHPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRAALHNEDVRETFCGRPVRTDRALIMAIVNRTPDSFYDRGATFTDEAAQAAVHQAVA
CCCCHHCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHH
DGADVVDIGGVKAGPGENVDADAEIARVVPFIEWVRGAHPDLLISVDTWRAEVAAKACAA
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHH
GADLINDTWAGADPELPAVAAAYGAGLVCSHTGGAVPRTRPYRVHYGTEVDGVVRAVLDE
CHHHHHCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHH
VTASAERALALGVSKDAILIDPTHDFGKNTFHGLALLRHVNDLVNTGWPVLMALSNKDFI
HHHHHHHHHHCCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHH
GETLGVGLTERLEGTLAATALAAAAGARMFRVHEVGPTRRTLEMVGSIVGTRPPARTVRG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCHHHHCC
LA
CC
>Mature Secondary Structure 
ARRAALHNEDVRETFCGRPVRTDRALIMAIVNRTPDSFYDRGATFTDEAAQAAVHQAVA
CCCHHCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHH
DGADVVDIGGVKAGPGENVDADAEIARVVPFIEWVRGAHPDLLISVDTWRAEVAAKACAA
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHH
GADLINDTWAGADPELPAVAAAYGAGLVCSHTGGAVPRTRPYRVHYGTEVDGVVRAVLDE
CHHHHHCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHH
VTASAERALALGVSKDAILIDPTHDFGKNTFHGLALLRHVNDLVNTGWPVLMALSNKDFI
HHHHHHHHHHCCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHH
GETLGVGLTERLEGTLAATALAAAAGARMFRVHEVGPTRRTLEMVGSIVGTRPPARTVRG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCHHHHCC
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate; 4-aminobenzoate

Specific reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]