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The map label for this gene is purU [H]

Identifier: 169628399

GI number: 169628399

Start: 1307662

End: 1308561

Strand: Direct

Name: purU [H]

Synonym: MAB_1306

Alternate gene names: 169628399

Gene position: 1307662-1308561 (Clockwise)

Preceding gene: 169628398

Following gene: 169628401

Centisome position: 25.81

GC content: 62.89

Gene sequence:

>900_bases
ATGTTCCCTCAAGGTATGCATCCCACCGGATCGTCAGATGCCTTCCATTCCAAGGACATCGGCAGGCTCCTGCTGCAGTG
CCCCGATCGGATCGGCGTGGTCGCGGCCGTGAGCGCCTTCCTGGCCGAAGCCGGCGCCAGCATTATCTCGCTGGCACAGT
ATTCGACGGAGCCGCAGGGCGGCTGGTTCATGCAGCGCACGGTGTTCCACCGTTCCGGGCTTGCCGCCGCGCGCGACGCG
ATGGAAGCCAAATTCGCTTCCATCGCCGAAGAATTCGACATTCAGTACCGATTCAGCGAGGCCGCCAAACCCAAACGAGT
GGCGATCATGGTCTCACGCACGGATCACTGCCTGCTGGACCTGCTGTGGCGTAATCGCCGCGGTGAGCTGGACATGTCGA
TCGCGATGGTGATCTCGAACCATCCCGACCTGGCCGACCAGGTGCGCTCGTTTGGGTTGCCCTTCGTGCACATCCCCGCA
ACCCGCGAGAACCGCGCCGACGCCGAACGCAAGCAGCTGGAACTGCTACAGGGAAACGTTGACCTGGTGGTGCTGGCCCG
CTACATGCAGATCCTGTCGCCGGAATTCCTCAACGAGATCGACTGTCCGCTGATCAACATCCACCATTCATTTCTTCCCG
CGTTCACCGGTGCCATGCCGTACCGGCGCGCTCGTGAGCGCGGTGTCAAAATGATCGGTGCCACAGCGCATTACGTGACT
GCTGAGCTCGACGAGGGGCCGATCATCGAGCAGGACGTGATCAGGGTGGACCACACCCACACCGTCGAAGACCTGGTGCG
GCTGGGTTCGGACGTGGAGCGCCTGGTGCTGTCGCGGGCAGTGGCCTGGCACTGCGAGGACCGCGTCATGCGGCACGGGA
ACGTCACCGCGATTCTCTGA

Upstream 100 bases:

>100_bases
GAGTAATGCTCCGATCATCATGACCGCACAGCGTCTGCTGCCCGCCCGGTTGTTCCTCAAGTTCCTGGCACGCATGCACG
GGCTGAAGGTGCGTTAGGTC

Downstream 100 bases:

>100_bases
TCCGGCGCGCGAGCGACACCTTAGGTCCGCTGAATGCGGCGTGTCTTTGTGCGCAACGCCGCGCTCGTGGCTGCTAGCGG
CCGATCTGCGTGTCGATGGT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MFPQGMHPTGSSDAFHSKDIGRLLLQCPDRIGVVAAVSAFLAEAGASIISLAQYSTEPQGGWFMQRTVFHRSGLAAARDA
MEAKFASIAEEFDIQYRFSEAAKPKRVAIMVSRTDHCLLDLLWRNRRGELDMSIAMVISNHPDLADQVRSFGLPFVHIPA
TRENRADAERKQLELLQGNVDLVVLARYMQILSPEFLNEIDCPLINIHHSFLPAFTGAMPYRRARERGVKMIGATAHYVT
AELDEGPIIEQDVIRVDHTHTVEDLVRLGSDVERLVLSRAVAWHCEDRVMRHGNVTAIL

Sequences:

>Translated_299_residues
MFPQGMHPTGSSDAFHSKDIGRLLLQCPDRIGVVAAVSAFLAEAGASIISLAQYSTEPQGGWFMQRTVFHRSGLAAARDA
MEAKFASIAEEFDIQYRFSEAAKPKRVAIMVSRTDHCLLDLLWRNRRGELDMSIAMVISNHPDLADQVRSFGLPFVHIPA
TRENRADAERKQLELLQGNVDLVVLARYMQILSPEFLNEIDCPLINIHHSFLPAFTGAMPYRRARERGVKMIGATAHYVT
AELDEGPIIEQDVIRVDHTHTVEDLVRLGSDVERLVLSRAVAWHCEDRVMRHGNVTAIL
>Mature_299_residues
MFPQGMHPTGSSDAFHSKDIGRLLLQCPDRIGVVAAVSAFLAEAGASIISLAQYSTEPQGGWFMQRTVFHRSGLAAARDA
MEAKFASIAEEFDIQYRFSEAAKPKRVAIMVSRTDHCLLDLLWRNRRGELDMSIAMVISNHPDLADQVRSFGLPFVHIPA
TRENRADAERKQLELLQGNVDLVVLARYMQILSPEFLNEIDCPLINIHHSFLPAFTGAMPYRRARERGVKMIGATAHYVT
AELDEGPIIEQDVIRVDHTHTVEDLVRLGSDVERLVLSRAVAWHCEDRVMRHGNVTAIL

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=184, Percent_Identity=32.6086956521739, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI209869995, Length=184, Percent_Identity=32.6086956521739, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI209869993, Length=184, Percent_Identity=32.6086956521739, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=42.2382671480144, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=27.4611398963731, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=190, Percent_Identity=32.6315789473684, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33479; Mature: 33479

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPQGMHPTGSSDAFHSKDIGRLLLQCPDRIGVVAAVSAFLAEAGASIISLAQYSTEPQG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC
GWFMQRTVFHRSGLAAARDAMEAKFASIAEEFDIQYRFSEAAKPKRVAIMVSRTDHCLLD
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCHHHHHH
LLWRNRRGELDMSIAMVISNHPDLADQVRSFGLPFVHIPATRENRADAERKQLELLQGNV
HHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCC
DLVVLARYMQILSPEFLNEIDCPLINIHHSFLPAFTGAMPYRRARERGVKMIGATAHYVT
HHHHHHHHHHHHCHHHHHHCCCCEEEHHHHHHHHHHCCCCHHHHHHCCCEEEECCHHEEE
AELDEGPIIEQDVIRVDHTHTVEDLVRLGSDVERLVLSRAVAWHCEDRVMRHGNVTAIL
EECCCCCCCHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MFPQGMHPTGSSDAFHSKDIGRLLLQCPDRIGVVAAVSAFLAEAGASIISLAQYSTEPQG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC
GWFMQRTVFHRSGLAAARDAMEAKFASIAEEFDIQYRFSEAAKPKRVAIMVSRTDHCLLD
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCHHHHHH
LLWRNRRGELDMSIAMVISNHPDLADQVRSFGLPFVHIPATRENRADAERKQLELLQGNV
HHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCC
DLVVLARYMQILSPEFLNEIDCPLINIHHSFLPAFTGAMPYRRARERGVKMIGATAHYVT
HHHHHHHHHHHHCHHHHHHCCCCEEEHHHHHHHHHHCCCCHHHHHHCCCEEEECCHHEEE
AELDEGPIIEQDVIRVDHTHTVEDLVRLGSDVERLVLSRAVAWHCEDRVMRHGNVTAIL
EECCCCCCCHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]