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The map label for this gene is gluQ [H]

Identifier: 169627383

GI number: 169627383

Start: 277369

End: 278244

Strand: Reverse

Name: gluQ [H]

Synonym: MAB_0278c

Alternate gene names: 169627383

Gene position: 278244-277369 (Counterclockwise)

Preceding gene: 169627384

Following gene: 169627382

Centisome position: 5.49

GC content: 67.24

Gene sequence:

>876_bases
GTGACCGGGGCAGGACGTTTCGCGCCGAGCCCGTCGGGCGATCTGCACATAGGAAATCTGCGGACGGCGCTGTTGGCGTG
GCTGCTCGCGCACTCGACGGGCCGCCGATTCCTGATGCGAGTGGAGGATCTGGATACCCGCACCAGCTCTGCGGTGGCGC
AGCGCCAGCTGGCCGACCTCGCCGCGATCGGAGTGCGGTGGGAGCTGCCCGTGGTGTGGCAGTCCGACCGCGCCCCCGCC
TACGACGAGGTGATAGCCTCGTTGGCCTCTCGCCACCTCCTCTACGAATGCTATTGCAGCAGAAAAGATATCGCGCAGGC
GCCGACTGCCCCGCATGCGCCACCCGGCGCCTATCCGGGCACGTGCCGCGACCTGTCGGAATCCGAACGTGCGCAGCGCC
GTTTAGACAGGGCACAGCGCCCACCTGCGCTGCGGCTGCGCACGGAAAACAGCCCGTTCACCGTGCAAGACCTACTGCAC
GGTGAATACACGGGCCTGGTCGACGATTTCGTGGTACGGCGCGGCGATGGCGTCACCGCCTACAACGTCGCAGTAGTGAT
CGATGACCACGCGCAGGGCATCGACCAGGTGGTGCGCGGCGAAGACCTGCTGGCCTCCGCTCCGCGGCAGGCCTATCTGG
CCGCGCTGCTGGGCTATCCGGCGCCTACCTATGCCCATGTGCCCTTGGTACTCAACACCGCAGGCGCACGATTGGCCAAG
CGCGACGGTGCGGTGACGCTCGCCGATCTCGGTGCGCAGCGGGCATTCGCGATGATCTGCGATTCTCTCGGGTACCAGGC
GGCCGACGCTACCGATCTGCTGCGCCAGTTCGATGCCGGACGTATCCCGCGCGAGCCGTGGATATACCCGGGCTGA

Upstream 100 bases:

>100_bases
TCAACCGCTGGCGCCCGGATCGCGACCCGCGATCCTGCACCTACGAGCAGCTAGAACAACCAATTACATTCAGCCTCAAC
GATATCGTTCCAGGTCTGGT

Downstream 100 bases:

>100_bases
TCCGAAGCTGCCTAGATGGACCCGAAACGTGTTAGCCTCCCGCGGTGTTTCGTGCCATCGTGCTTGCGCTTCTGGCGCTT
GTGGCGGTAGCCGGATGTGC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTGAGRFAPSPSGDLHIGNLRTALLAWLLAHSTGRRFLMRVEDLDTRTSSAVAQRQLADLAAIGVRWELPVVWQSDRAPA
YDEVIASLASRHLLYECYCSRKDIAQAPTAPHAPPGAYPGTCRDLSESERAQRRLDRAQRPPALRLRTENSPFTVQDLLH
GEYTGLVDDFVVRRGDGVTAYNVAVVIDDHAQGIDQVVRGEDLLASAPRQAYLAALLGYPAPTYAHVPLVLNTAGARLAK
RDGAVTLADLGAQRAFAMICDSLGYQAADATDLLRQFDAGRIPREPWIYPG

Sequences:

>Translated_291_residues
MTGAGRFAPSPSGDLHIGNLRTALLAWLLAHSTGRRFLMRVEDLDTRTSSAVAQRQLADLAAIGVRWELPVVWQSDRAPA
YDEVIASLASRHLLYECYCSRKDIAQAPTAPHAPPGAYPGTCRDLSESERAQRRLDRAQRPPALRLRTENSPFTVQDLLH
GEYTGLVDDFVVRRGDGVTAYNVAVVIDDHAQGIDQVVRGEDLLASAPRQAYLAALLGYPAPTYAHVPLVLNTAGARLAK
RDGAVTLADLGAQRAFAMICDSLGYQAADATDLLRQFDAGRIPREPWIYPG
>Mature_290_residues
TGAGRFAPSPSGDLHIGNLRTALLAWLLAHSTGRRFLMRVEDLDTRTSSAVAQRQLADLAAIGVRWELPVVWQSDRAPAY
DEVIASLASRHLLYECYCSRKDIAQAPTAPHAPPGAYPGTCRDLSESERAQRRLDRAQRPPALRLRTENSPFTVQDLLHG
EYTGLVDDFVVRRGDGVTAYNVAVVIDDHAQGIDQVVRGEDLLASAPRQAYLAALLGYPAPTYAHVPLVLNTAGARLAKR
DGAVTLADLGAQRAFAMICDSLGYQAADATDLLRQFDAGRIPREPWIYPG

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=263, Percent_Identity=34.6007604562738, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI226510925, Length=268, Percent_Identity=38.4328358208955, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1788743, Length=255, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71993394, Length=252, Percent_Identity=28.968253968254, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324540, Length=278, Percent_Identity=32.0143884892086, Blast_Score=145, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24665321, Length=263, Percent_Identity=32.6996197718631, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 31709; Mature: 31578

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAGRFAPSPSGDLHIGNLRTALLAWLLAHSTGRRFLMRVEDLDTRTSSAVAQRQLADL
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCHHEEHHHHHHCCHHHHHHHHHHHHHH
AAIGVRWELPVVWQSDRAPAYDEVIASLASRHLLYECYCSRKDIAQAPTAPHAPPGAYPG
HHHCCEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCC
TCRDLSESERAQRRLDRAQRPPALRLRTENSPFTVQDLLHGEYTGLVDDFVVRRGDGVTA
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHCCHHHHHHHHHHHHCCCCCEE
YNVAVVIDDHAQGIDQVVRGEDLLASAPRQAYLAALLGYPAPTYAHVPLVLNTAGARLAK
EEEEEEECCHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCCEECCEEEECCCCHHHH
RDGAVTLADLGAQRAFAMICDSLGYQAADATDLLRQFDAGRIPREPWIYPG
CCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGAGRFAPSPSGDLHIGNLRTALLAWLLAHSTGRRFLMRVEDLDTRTSSAVAQRQLADL
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCHHEEHHHHHHCCHHHHHHHHHHHHHH
AAIGVRWELPVVWQSDRAPAYDEVIASLASRHLLYECYCSRKDIAQAPTAPHAPPGAYPG
HHHCCEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCC
TCRDLSESERAQRRLDRAQRPPALRLRTENSPFTVQDLLHGEYTGLVDDFVVRRGDGVTA
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHCCHHHHHHHHHHHHCCCCCEE
YNVAVVIDDHAQGIDQVVRGEDLLASAPRQAYLAALLGYPAPTYAHVPLVLNTAGARLAK
EEEEEEECCHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCCEECCEEEECCCCHHHH
RDGAVTLADLGAQRAFAMICDSLGYQAADATDLLRQFDAGRIPREPWIYPG
CCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA