BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is hdhA [C]

Identifier: 169627364

GI number: 169627364

Start: 261527

End: 262309

Strand: Reverse

Name: hdhA [C]

Synonym: MAB_0259c

Alternate gene names: 169627364

Gene position: 262309-261527 (Counterclockwise)

Preceding gene: 169627371

Following gene: 169627363

Centisome position: 5.18

GC content: 63.09

Gene sequence:

>783_bases
ATGACAGGACTTCTTTCCGGAAAAACGGTGGTGATATCCGGAGTGGGCACCGGATTGGGTCGCGAGGTCGCGCTGAAGGC
ACATCAAGACGGTGCGAACGTGGTGCTCGGCGCCCGCACGCGGGAGAACCTCGAAAGGCTCACGACCGAACTCGATGACA
CGGGCGGTTCCGCCACATGGGCGGTCACCGACATCACCGACGCGACCGCATGCCAGGGCCTCATCGATACCGCTGTCAAG
AGATTCGGCGCCGTGGACGCGCTGGTCAATGTCGCCGCCAAAGAGGACGTGTTCGGTGGCATCGAGGGGGCGGACCTGGC
GCAATGGCAGGCGATGCTGAACACCAACGTGGTCGGCACGCTGCAGCTGGTACAGGCCGCGGTCCCGGAGCTGAAGAAAA
AGGGCGGGTCGGTGGTTTTCATCGGTTCGCAATCGGCGTTCCATCCGCAACTGCCGCAGTCCGCGTACGCGACGTCCAAG
GGCGCGCTGCAGAACGCGATGTATCAGTTGGCGAAGGAACTCGGGCCACACAAGATCCGTCTCAACATGGTGGTGCCGAC
GTGGATGTACGGCCCGCCCATTCAGCTGTACATCGGCCTGGTCGCTCAGCAGCGCGGCATCACCCAGGAGGAGGTGCTCG
CGGAACTGACGGCCAAATTCCCCTTGGGCGAGATGCCTGCCGACGAAGACGTCGCCGACGCGGTGGTGTTCTTCGTGTCG
GACCGAGCCCGAATGGTGACCGGGCAGACCCTATTCGTCAATGGTGGCGAGTTTTTCCGATGA

Upstream 100 bases:

>100_bases
CGGCGACGGCCCCTGCCCAAGGATGACTCTTCGGTACTCATTGCAAATATCGTAACACTGTGTAACGTTGAGGCGTGGGT
CACAGACAGAAGGGCAGCCG

Downstream 100 bases:

>100_bases
GCGCTTGCGCGAAGAGAGTGAGCAGAGCATGAGTGATAAGTATCCCGTCATCGTTTGGGGCACAGGAGTTGTCGGCAAGC
TGGTAATCCGCGAATTACTG

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTGLLSGKTVVISGVGTGLGREVALKAHQDGANVVLGARTRENLERLTTELDDTGGSATWAVTDITDATACQGLIDTAVK
RFGAVDALVNVAAKEDVFGGIEGADLAQWQAMLNTNVVGTLQLVQAAVPELKKKGGSVVFIGSQSAFHPQLPQSAYATSK
GALQNAMYQLAKELGPHKIRLNMVVPTWMYGPPIQLYIGLVAQQRGITQEEVLAELTAKFPLGEMPADEDVADAVVFFVS
DRARMVTGQTLFVNGGEFFR

Sequences:

>Translated_260_residues
MTGLLSGKTVVISGVGTGLGREVALKAHQDGANVVLGARTRENLERLTTELDDTGGSATWAVTDITDATACQGLIDTAVK
RFGAVDALVNVAAKEDVFGGIEGADLAQWQAMLNTNVVGTLQLVQAAVPELKKKGGSVVFIGSQSAFHPQLPQSAYATSK
GALQNAMYQLAKELGPHKIRLNMVVPTWMYGPPIQLYIGLVAQQRGITQEEVLAELTAKFPLGEMPADEDVADAVVFFVS
DRARMVTGQTLFVNGGEFFR
>Mature_259_residues
TGLLSGKTVVISGVGTGLGREVALKAHQDGANVVLGARTRENLERLTTELDDTGGSATWAVTDITDATACQGLIDTAVKR
FGAVDALVNVAAKEDVFGGIEGADLAQWQAMLNTNVVGTLQLVQAAVPELKKKGGSVVFIGSQSAFHPQLPQSAYATSKG
ALQNAMYQLAKELGPHKIRLNMVVPTWMYGPPIQLYIGLVAQQRGITQEEVLAELTAKFPLGEMPADEDVADAVVFFVSD
RARMVTGQTLFVNGGEFFR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=257, Percent_Identity=33.4630350194552, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI5031737, Length=253, Percent_Identity=28.0632411067194, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI33667109, Length=184, Percent_Identity=30.9782608695652, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI7705925, Length=258, Percent_Identity=31.3953488372093, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI126723191, Length=181, Percent_Identity=34.2541436464088, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI126723750, Length=257, Percent_Identity=30.7392996108949, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI59889578, Length=228, Percent_Identity=28.5087719298246, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI40254986, Length=139, Percent_Identity=33.8129496402878, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI40254992, Length=252, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI15277342, Length=260, Percent_Identity=27.3076923076923, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI19923817, Length=265, Percent_Identity=26.0377358490566, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=26.2548262548263, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4503817, Length=237, Percent_Identity=24.8945147679325, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI32455239, Length=185, Percent_Identity=26.4864864864865, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5031765, Length=185, Percent_Identity=26.4864864864865, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI302344755, Length=108, Percent_Identity=39.8148148148148, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI20149619, Length=195, Percent_Identity=31.7948717948718, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI7657478, Length=203, Percent_Identity=32.0197044334975, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787905, Length=255, Percent_Identity=29.8039215686275, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787335, Length=262, Percent_Identity=28.2442748091603, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=27.34375, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789208, Length=262, Percent_Identity=29.0076335877863, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082160, Length=258, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786812, Length=260, Percent_Identity=29.2307692307692, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788459, Length=257, Percent_Identity=26.8482490272374, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787526, Length=187, Percent_Identity=28.3422459893048, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=24.901185770751, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367365, Length=238, Percent_Identity=31.0924369747899, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=265, Percent_Identity=29.811320754717, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=29.5880149812734, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=265, Percent_Identity=27.1698113207547, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=25.5905511811024, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538480, Length=268, Percent_Identity=28.7313432835821, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982365, Length=200, Percent_Identity=29, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=261, Percent_Identity=26.4367816091954, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17560220, Length=270, Percent_Identity=28.8888888888889, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17562904, Length=270, Percent_Identity=28.8888888888889, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17558628, Length=195, Percent_Identity=31.7948717948718, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560332, Length=272, Percent_Identity=28.6764705882353, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=25.0996015936255, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=257, Percent_Identity=28.0155642023346, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=268, Percent_Identity=28.7313432835821, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=26.5151515151515, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=251, Percent_Identity=25.0996015936255, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=275, Percent_Identity=28.3636363636364, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=26.5917602996255, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=273, Percent_Identity=26.7399267399267, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=273, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17551412, Length=276, Percent_Identity=26.8115942028986, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=261, Percent_Identity=26.0536398467433, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17508651, Length=252, Percent_Identity=24.2063492063492, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=271, Percent_Identity=26.1992619926199, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=26.2357414448669, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=273, Percent_Identity=27.8388278388278, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17565030, Length=278, Percent_Identity=25.5395683453237, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=197, Percent_Identity=26.3959390862944, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=260, Percent_Identity=25.7692307692308, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322226, Length=237, Percent_Identity=27.8481012658228, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323882, Length=257, Percent_Identity=30.3501945525292, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322227, Length=188, Percent_Identity=28.1914893617021, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324126, Length=223, Percent_Identity=23.7668161434978, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI23397609, Length=255, Percent_Identity=31.7647058823529, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355319, Length=256, Percent_Identity=26.953125, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357673, Length=209, Percent_Identity=29.1866028708134, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571526, Length=255, Percent_Identity=28.6274509803922, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24644339, Length=255, Percent_Identity=30.1960784313725, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=27.9527559055118, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639444, Length=258, Percent_Identity=25.1937984496124, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24643142, Length=264, Percent_Identity=25.3787878787879, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21358495, Length=195, Percent_Identity=28.7179487179487, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859837, Length=197, Percent_Identity=29.4416243654822, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17737361, Length=201, Percent_Identity=25.8706467661692, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27590; Mature: 27459

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLLSGKTVVISGVGTGLGREVALKAHQDGANVVLGARTRENLERLTTELDDTGGSATW
CCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEE
AVTDITDATACQGLIDTAVKRFGAVDALVNVAAKEDVFGGIEGADLAQWQAMLNTNVVGT
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHH
LQLVQAAVPELKKKGGSVVFIGSQSAFHPQLPQSAYATSKGALQNAMYQLAKELGPHKIR
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEE
LNMVVPTWMYGPPIQLYIGLVAQQRGITQEEVLAELTAKFPLGEMPADEDVADAVVFFVS
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEC
DRARMVTGQTLFVNGGEFFR
CCCEEEECCEEEECCCCCCC
>Mature Secondary Structure 
TGLLSGKTVVISGVGTGLGREVALKAHQDGANVVLGARTRENLERLTTELDDTGGSATW
CCCCCCCEEEEEECCCCCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEE
AVTDITDATACQGLIDTAVKRFGAVDALVNVAAKEDVFGGIEGADLAQWQAMLNTNVVGT
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHH
LQLVQAAVPELKKKGGSVVFIGSQSAFHPQLPQSAYATSKGALQNAMYQLAKELGPHKIR
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEE
LNMVVPTWMYGPPIQLYIGLVAQQRGITQEEVLAELTAKFPLGEMPADEDVADAVVFFVS
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEC
DRARMVTGQTLFVNGGEFFR
CCCEEEECCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]