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The map label for this gene is sseB [H]

Identifier: 169627176

GI number: 169627176

Start: 67570

End: 68406

Strand: Reverse

Name: sseB [H]

Synonym: MAB_0071c

Alternate gene names: 169627176

Gene position: 68406-67570 (Counterclockwise)

Preceding gene: 169627177

Following gene: 169627171

Centisome position: 1.35

GC content: 69.77

Gene sequence:

>837_bases
ATGGACGTGCTCATCTCGCCCTCCGAACTCGCTGACCGGCTAACCGACTTCCGGCTGCTGGACGTGCGCTGGACCGTCAT
GGCGCCGGACGGTCGCGCGGCCTACCTGGCGGGTCACCTTCCCGGCGCGGTGTTCGTGGATCTCGACGTCGACCTCGCCG
ATCACTCCGTCACGGGTCGCGGGCGCCATCCGCTGCCCACACCGCAGGCTCTTGCGGCCAGCGCCCGCCGCTGGGGTCTG
AACGACGGCGACGCCGTGGTGGTTTACGACGATTGGAACGGGCAGGCAGCTGCGCGCGCCTGGTGGCTGCTGCGCGCGGC
GGGCGTCTCGAATGTCCGGATACTCGATGGCGGCTGGGCCGCCTGGCAGCGGTCGGGTGGTCCGGTCGCGACGGAGGAGG
TTGTCCCCGTGGTGGGCGACATCACCATCACCTCGCTGCACGGTCTTGCCGCCGTCGACGCCGATGCGGTTGCCGCTCAA
GCCCAATCACCAGACAATCTGGTATTCGACGCACGCGCAGCGGCCCGCTATCGCGGCGATGAAGAACCCCTGGACCCTCG
TGCCGGGCACATCCCTGGTGCCGTGTCCGCTCCCACCGCCGAGAACCTGACCGCGGACGGCACCTTCAAACCGGTAGCCG
AGTTGCGTGAACGCTTCGACAAGTTGGGTGCGGGTGCCGCACCCGTCACGGTGTACTGCGGCTCGGGCGTCACCGCCACC
CATCAGATCGCGGCGCTTGCCATCGCCGGATACGACGCGGCGCTCTACCCGGGCTCGTGGTCGGAGTGGTCGAGCGACCC
GCAACGCCCCGTCGCGACGGGTCCCAACCCGGCCTAG

Upstream 100 bases:

>100_bases
CCTGGCCTCGGACGAGGCGCGGTACATCACCTCGGTGAGCCTTCCCGTCGACGCGGGCAGCACCCAACGATAGGAGCCAT
CAGCGCGTACCGTCACCGGT

Downstream 100 bases:

>100_bases
AGCAGTCCGGCTGCCAGCGCGATGATTCCCAGCAGCGGTGGTATCAACTGCTTCAGGGCCGCGGAGGCCTTATCGGGCGA
CGAAATCAGCAGCACCAGCC

Product: putative thiosulfate sulfurtransferase (SseB)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MDVLISPSELADRLTDFRLLDVRWTVMAPDGRAAYLAGHLPGAVFVDLDVDLADHSVTGRGRHPLPTPQALAASARRWGL
NDGDAVVVYDDWNGQAAARAWWLLRAAGVSNVRILDGGWAAWQRSGGPVATEEVVPVVGDITITSLHGLAAVDADAVAAQ
AQSPDNLVFDARAAARYRGDEEPLDPRAGHIPGAVSAPTAENLTADGTFKPVAELRERFDKLGAGAAPVTVYCGSGVTAT
HQIAALAIAGYDAALYPGSWSEWSSDPQRPVATGPNPA

Sequences:

>Translated_278_residues
MDVLISPSELADRLTDFRLLDVRWTVMAPDGRAAYLAGHLPGAVFVDLDVDLADHSVTGRGRHPLPTPQALAASARRWGL
NDGDAVVVYDDWNGQAAARAWWLLRAAGVSNVRILDGGWAAWQRSGGPVATEEVVPVVGDITITSLHGLAAVDADAVAAQ
AQSPDNLVFDARAAARYRGDEEPLDPRAGHIPGAVSAPTAENLTADGTFKPVAELRERFDKLGAGAAPVTVYCGSGVTAT
HQIAALAIAGYDAALYPGSWSEWSSDPQRPVATGPNPA
>Mature_278_residues
MDVLISPSELADRLTDFRLLDVRWTVMAPDGRAAYLAGHLPGAVFVDLDVDLADHSVTGRGRHPLPTPQALAASARRWGL
NDGDAVVVYDDWNGQAAARAWWLLRAAGVSNVRILDGGWAAWQRSGGPVATEEVVPVVGDITITSLHGLAAVDADAVAAQ
AQSPDNLVFDARAAARYRGDEEPLDPRAGHIPGAVSAPTAENLTADGTFKPVAELRERFDKLGAGAAPVTVYCGSGVTAT
HQIAALAIAGYDAALYPGSWSEWSSDPQRPVATGPNPA

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=261, Percent_Identity=33.7164750957854, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI61835204, Length=261, Percent_Identity=33.7164750957854, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194473681, Length=261, Percent_Identity=33.7164750957854, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI17402865, Length=272, Percent_Identity=33.0882352941176, Blast_Score=110, Evalue=9e-25,
Organism=Escherichia coli, GI87082121, Length=271, Percent_Identity=36.5313653136531, Blast_Score=134, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17561888, Length=264, Percent_Identity=31.0606060606061, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71997283, Length=274, Percent_Identity=30.6569343065693, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17543836, Length=296, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543838, Length=296, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559150, Length=264, Percent_Identity=25.7575757575758, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 29142; Mature: 29142

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLISPSELADRLTDFRLLDVRWTVMAPDGRAAYLAGHLPGAVFVDLDVDLADHSVTGR
CCEEECHHHHHHHHHCEEEEEEEEEEECCCCCEEEEEECCCCEEEEEEECEECCCCCCCC
GRHPLPTPQALAASARRWGLNDGDAVVVYDDWNGQAAARAWWLLRAAGVSNVRILDGGWA
CCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCHH
AWQRSGGPVATEEVVPVVGDITITSLHGLAAVDADAVAAQAQSPDNLVFDARAAARYRGD
HHHCCCCCCCHHHHCEEECCEEEEEECCEECCCHHHHHHHCCCCCCEEEECHHHHHHCCC
EEPLDPRAGHIPGAVSAPTAENLTADGTFKPVAELRERFDKLGAGAAPVTVYCGSGVTAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHH
HQIAALAIAGYDAALYPGSWSEWSSDPQRPVATGPNPA
HHEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDVLISPSELADRLTDFRLLDVRWTVMAPDGRAAYLAGHLPGAVFVDLDVDLADHSVTGR
CCEEECHHHHHHHHHCEEEEEEEEEEECCCCCEEEEEECCCCEEEEEEECEECCCCCCCC
GRHPLPTPQALAASARRWGLNDGDAVVVYDDWNGQAAARAWWLLRAAGVSNVRILDGGWA
CCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCHH
AWQRSGGPVATEEVVPVVGDITITSLHGLAAVDADAVAAQAQSPDNLVFDARAAARYRGD
HHHCCCCCCCHHHHCEEECCEEEEEECCEECCCHHHHHHHCCCCCCEEEECHHHHHHCCC
EEPLDPRAGHIPGAVSAPTAENLTADGTFKPVAELRERFDKLGAGAAPVTVYCGSGVTAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHH
HQIAALAIAGYDAALYPGSWSEWSSDPQRPVATGPNPA
HHEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]