Click here to switch to the map view.

The map label for this gene is sipA [H]

Identifier: 16766188

GI number: 16766188

Start: 3024679

End: 3026736

Strand: Reverse

Name: sipA [H]

Synonym: STM2882

Alternate gene names: 16766188

Gene position: 3026736-3024679 (Counterclockwise)

Preceding gene: 16766189

Following gene: 16766187

Centisome position: 62.31

GC content: 48.01

Gene sequence:

>2058_bases
ATGGTTACAAGTGTAAGGACTCAGCCCCCCGTCATAATGCCAGGTATGCAGACCGAGATCAAAACGCAGGCCACGAATCT
TGCGGCGAATCTTTCCGCAGTCAGAGAAAGTGCCACAGCGACGCTGTCAGGGGAAATTAAAGGCCCGCAACTGGAAGATT
TTCCCGCGCTGATCAAACAGGCGAGTCTGGATGCATTGTTTAAATGCGGGAAAGACGCTGAGGCGTTAAAAGAAGTTTTT
ACCAATTCAAATAATGTCGCCGGTAAGAAAGCGATAATGGAGTTTGCCGGGCTCTTTCGTTCAGCGCTCAACGCCACCAG
TGATTCTCCTGAGGCGAAGACGCTACTGATGAAGGTGGGGGCAGAGTATACCGCGCAAATCATAAAAGATGGCCTGAAAG
AAAAGTCAGCTTTTGGGCCATGGCTGCCAGAAACAAAGAAAGCGGAAGCGAAGCTGGAAAACCTGGAAAAGCAGCTGTTA
GATATTATCAAAAATAACACTGGCGGTGAATTAAGTAAATTATCGACGAATCTTGTTATGCAGGAGGTGATGCCCTATAT
TGCCAGCTGCATTGAACATAACTTTGGCTGTACGTTAGATCCGTTAACCCGCAGCAATCTTACGCACCTTGTTGACAAAG
CGGCGGCGAAGGCTGTTGAGGCGCTTGATATGTGCCACCAAAAATTAACGCAAGAGCAGGGTACCAGCGTAGGACGGGAA
GCCCGGCACCTTGAAATGCAAACGTTGATACCCCTGCTGCTACGTAATGTTTTTGCACAAATTCCTGCAGATAAACTGCC
TGACCCTAAAATTCCGGAGCCTGCGGCTGGACCAGTACCTGATGGTGGGAAAAAAGCAGAACCTACGGGTATTAATATCA
ATATTAATATTGATAGCAGTAACCATAGCGTGGATAACAGTAAGCATATTAACAATAGCCGAAGCCATGTCGATAATAGC
CAGCGCCATATCGATAATAGCAACCATGATAATAGCCGGAAGACGATTGATAATAGCCGAACATTTATTGATAACAGCCA
ACGCAATGGCGAGTCACACCATTCGACTAACAGCAGCAACGTAAGTCATAGTCATTCGCGTGTGGATTCGACTACGCATC
AAACGGAGACGGCACACAGCGCCAGCACAGGGGCAATTGACCATGGCATCGCGGGTAAAATTGACGTCACGGCTCATGCG
ACGGCAGAGGCTGTGACCAACGCTTCATCCGAAAGTAAAGATGGAAAGGTGGTCACGTCAGAAAAGGGCACTACGGGTGA
AACAACCTCTTTTGATGAAGTCGATGGCGTAACCAGCAAGAGCATTATCGGCAAGCCGGTACAGGCCACGGTGCACGGCG
TTGATGATAATAAACAGCAAAGCCAGACGGCAGAGATTGTGAATGTGAAGCCGCTTGCCAGTCAACTGGCTGGCGTCGAG
AATGTTAAAACCGATACCTTACAATCAGACACGACGGTAATTACAGGTAATAAAGCCGGTACGACCGATAATGATAATAG
TCAGACAGATAAGACTGGCCCATTTTCTGGTTTGAAGTTTAAGCAAAATAGTTTCCTCTCAACAGTACCGAGCGTTACGA
ATATGCATTCAATGCATTTCGACGCCCGTGAGACGTTTTTGGGTGTGATACGTAAAGCCCTGGAGCCAGATACCTCCACA
CCGTTCCCTGTACGCAGAGCGTTTGACGGCTTGCGTGCGGAAATATTACCCAATGACACGATAAAGAGTGCAGCGTTGAA
AGCGCAATGTAGCGATATTGACAAGCACCCAGAATTGAAAGCGAAAATGGAGACTCTCAAAGAGGTCATTACTCATCATC
CACAAAAAGAAAAACTGGCTGAGATTGCACTGCAGTTTGCCAGAGAGGCGGGGCTGACCAGGCTAAAAGGGGAAACTGAC
TATGTGTTAAGTAATGTGCTGGACGGCCTTATCGGAGACGGTAGCTGGCGAGCTGGCCCGGCTTACGAGTCATACCTGAA
TAAACCTGGCGTGGATCGGGTTATTACTACCGTTGATGGCTTGCACATGCAGCGTTAA

Upstream 100 bases:

>100_bases
ATTGTACGACAACCTGGTAAAAGTGCTGAGCAGTACGATAAGTAGCAGCCTGGAAACCGCCAAAAGCTTCCTGCAAGGAT
AACAGAAGAGGATATTAATA

Downstream 100 bases:

>100_bases
TTAACCGGGAAAGATGCGATGAATATGGATATTGAAGCAAGAGTCAAAAAAGTGATCACCTCTTGTATTGCCGTTGATGT
TGATAGTATCAATGGTCAGA

Product: secreted effector protein

Products: NA

Alternate protein names: Effector protein sipA [H]

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MVTSVRTQPPVIMPGMQTEIKTQATNLAANLSAVRESATATLSGEIKGPQLEDFPALIKQASLDALFKCGKDAEALKEVF
TNSNNVAGKKAIMEFAGLFRSALNATSDSPEAKTLLMKVGAEYTAQIIKDGLKEKSAFGPWLPETKKAEAKLENLEKQLL
DIIKNNTGGELSKLSTNLVMQEVMPYIASCIEHNFGCTLDPLTRSNLTHLVDKAAAKAVEALDMCHQKLTQEQGTSVGRE
ARHLEMQTLIPLLLRNVFAQIPADKLPDPKIPEPAAGPVPDGGKKAEPTGINININIDSSNHSVDNSKHINNSRSHVDNS
QRHIDNSNHDNSRKTIDNSRTFIDNSQRNGESHHSTNSSNVSHSHSRVDSTTHQTETAHSASTGAIDHGIAGKIDVTAHA
TAEAVTNASSESKDGKVVTSEKGTTGETTSFDEVDGVTSKSIIGKPVQATVHGVDDNKQQSQTAEIVNVKPLASQLAGVE
NVKTDTLQSDTTVITGNKAGTTDNDNSQTDKTGPFSGLKFKQNSFLSTVPSVTNMHSMHFDARETFLGVIRKALEPDTST
PFPVRRAFDGLRAEILPNDTIKSAALKAQCSDIDKHPELKAKMETLKEVITHHPQKEKLAEIALQFAREAGLTRLKGETD
YVLSNVLDGLIGDGSWRAGPAYESYLNKPGVDRVITTVDGLHMQR

Sequences:

>Translated_685_residues
MVTSVRTQPPVIMPGMQTEIKTQATNLAANLSAVRESATATLSGEIKGPQLEDFPALIKQASLDALFKCGKDAEALKEVF
TNSNNVAGKKAIMEFAGLFRSALNATSDSPEAKTLLMKVGAEYTAQIIKDGLKEKSAFGPWLPETKKAEAKLENLEKQLL
DIIKNNTGGELSKLSTNLVMQEVMPYIASCIEHNFGCTLDPLTRSNLTHLVDKAAAKAVEALDMCHQKLTQEQGTSVGRE
ARHLEMQTLIPLLLRNVFAQIPADKLPDPKIPEPAAGPVPDGGKKAEPTGINININIDSSNHSVDNSKHINNSRSHVDNS
QRHIDNSNHDNSRKTIDNSRTFIDNSQRNGESHHSTNSSNVSHSHSRVDSTTHQTETAHSASTGAIDHGIAGKIDVTAHA
TAEAVTNASSESKDGKVVTSEKGTTGETTSFDEVDGVTSKSIIGKPVQATVHGVDDNKQQSQTAEIVNVKPLASQLAGVE
NVKTDTLQSDTTVITGNKAGTTDNDNSQTDKTGPFSGLKFKQNSFLSTVPSVTNMHSMHFDARETFLGVIRKALEPDTST
PFPVRRAFDGLRAEILPNDTIKSAALKAQCSDIDKHPELKAKMETLKEVITHHPQKEKLAEIALQFAREAGLTRLKGETD
YVLSNVLDGLIGDGSWRAGPAYESYLNKPGVDRVITTVDGLHMQR
>Mature_685_residues
MVTSVRTQPPVIMPGMQTEIKTQATNLAANLSAVRESATATLSGEIKGPQLEDFPALIKQASLDALFKCGKDAEALKEVF
TNSNNVAGKKAIMEFAGLFRSALNATSDSPEAKTLLMKVGAEYTAQIIKDGLKEKSAFGPWLPETKKAEAKLENLEKQLL
DIIKNNTGGELSKLSTNLVMQEVMPYIASCIEHNFGCTLDPLTRSNLTHLVDKAAAKAVEALDMCHQKLTQEQGTSVGRE
ARHLEMQTLIPLLLRNVFAQIPADKLPDPKIPEPAAGPVPDGGKKAEPTGINININIDSSNHSVDNSKHINNSRSHVDNS
QRHIDNSNHDNSRKTIDNSRTFIDNSQRNGESHHSTNSSNVSHSHSRVDSTTHQTETAHSASTGAIDHGIAGKIDVTAHA
TAEAVTNASSESKDGKVVTSEKGTTGETTSFDEVDGVTSKSIIGKPVQATVHGVDDNKQQSQTAEIVNVKPLASQLAGVE
NVKTDTLQSDTTVITGNKAGTTDNDNSQTDKTGPFSGLKFKQNSFLSTVPSVTNMHSMHFDARETFLGVIRKALEPDTST
PFPVRRAFDGLRAEILPNDTIKSAALKAQCSDIDKHPELKAKMETLKEVITHHPQKEKLAEIALQFAREAGLTRLKGETD
YVLSNVLDGLIGDGSWRAGPAYESYLNKPGVDRVITTVDGLHMQR

Specific function: Actin-binding protein that interferes with host cell actin cytoskeleton. It stimulates actin polymerization and counteracts F-actin destabilizing proteins. Potentiates sipC activity; both are required for an efficient bacterial internalization [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the type III secretion system 1 (SPI-1 TTSS) (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sipA/ipaA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015138
- InterPro:   IPR023224
- InterPro:   IPR023225 [H]

Pfam domain/function: PF09052 SipA [H]

EC number: NA

Molecular weight: Translated: 73943; Mature: 73943

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSVRTQPPVIMPGMQTEIKTQATNLAANLSAVRESATATLSGEIKGPQLEDFPALIKQ
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
ASLDALFKCGKDAEALKEVFTNSNNVAGKKAIMEFAGLFRSALNATSDSPEAKTLLMKVG
HHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AEYTAQIIKDGLKEKSAFGPWLPETKKAEAKLENLEKQLLDIIKNNTGGELSKLSTNLVM
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
QEVMPYIASCIEHNFGCTLDPLTRSNLTHLVDKAAAKAVEALDMCHQKLTQEQGTSVGRE
HHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ARHLEMQTLIPLLLRNVFAQIPADKLPDPKIPEPAAGPVPDGGKKAEPTGINININIDSS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
NHSVDNSKHINNSRSHVDNSQRHIDNSNHDNSRKTIDNSRTFIDNSQRNGESHHSTNSSN
CCCCCCCHHCCCCHHHCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
VSHSHSRVDSTTHQTETAHSASTGAIDHGIAGKIDVTAHATAEAVTNASSESKDGKVVTS
CCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCEEEC
EKGTTGETTSFDEVDGVTSKSIIGKPVQATVHGVDDNKQQSQTAEIVNVKPLASQLAGVE
CCCCCCCCCCCHHHCCCCHHHHCCCCHHHEEECCCCCHHHHHHHHHHCCHHHHHHHCCCC
NVKTDTLQSDTTVITGNKAGTTDNDNSQTDKTGPFSGLKFKQNSFLSTVPSVTNMHSMHF
CCCHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHCCCHHHHHHHCC
DARETFLGVIRKALEPDTSTPFPVRRAFDGLRAEILPNDTIKSAALKAQCSDIDKHPELK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHH
AKMETLKEVITHHPQKEKLAEIALQFAREAGLTRLKGETDYVLSNVLDGLIGDGSWRAGP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCCCC
AYESYLNKPGVDRVITTVDGLHMQR
HHHHHCCCCCHHHHHHHHCCHHCCC
>Mature Secondary Structure
MVTSVRTQPPVIMPGMQTEIKTQATNLAANLSAVRESATATLSGEIKGPQLEDFPALIKQ
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHH
ASLDALFKCGKDAEALKEVFTNSNNVAGKKAIMEFAGLFRSALNATSDSPEAKTLLMKVG
HHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AEYTAQIIKDGLKEKSAFGPWLPETKKAEAKLENLEKQLLDIIKNNTGGELSKLSTNLVM
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
QEVMPYIASCIEHNFGCTLDPLTRSNLTHLVDKAAAKAVEALDMCHQKLTQEQGTSVGRE
HHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ARHLEMQTLIPLLLRNVFAQIPADKLPDPKIPEPAAGPVPDGGKKAEPTGINININIDSS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
NHSVDNSKHINNSRSHVDNSQRHIDNSNHDNSRKTIDNSRTFIDNSQRNGESHHSTNSSN
CCCCCCCHHCCCCHHHCCCHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
VSHSHSRVDSTTHQTETAHSASTGAIDHGIAGKIDVTAHATAEAVTNASSESKDGKVVTS
CCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCEEEC
EKGTTGETTSFDEVDGVTSKSIIGKPVQATVHGVDDNKQQSQTAEIVNVKPLASQLAGVE
CCCCCCCCCCCHHHCCCCHHHHCCCCHHHEEECCCCCHHHHHHHHHHCCHHHHHHHCCCC
NVKTDTLQSDTTVITGNKAGTTDNDNSQTDKTGPFSGLKFKQNSFLSTVPSVTNMHSMHF
CCCHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHCCCHHHHHHHCC
DARETFLGVIRKALEPDTSTPFPVRRAFDGLRAEILPNDTIKSAALKAQCSDIDKHPELK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHH
AKMETLKEVITHHPQKEKLAEIALQFAREAGLTRLKGETDYVLSNVLDGLIGDGSWRAGP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCCCC
AYESYLNKPGVDRVITTVDGLHMQR
HHHHHCCCCCHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA