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The map label for this gene is rfbM

Identifier: 16765414

GI number: 16765414

Start: 2163853

End: 2165292

Strand: Reverse

Name: rfbM

Synonym: STM2084

Alternate gene names: 16765414

Gene position: 2165292-2163853 (Counterclockwise)

Preceding gene: 16765415

Following gene: 16765413

Centisome position: 44.58

GC content: 40.28

Gene sequence:

>1440_bases
ATGTCTTTTCTTCCCGTAATTATGGCTGGCGGCACAGGTAGCCGTTTATGGCCGCTTTCACGCGAATATCATCCGAAGCA
GTTTCTAAGCGTTGAAGGTAAACTATCAATGCTGCAAAATACTATAAAGCGATTAGCTTCACTTTCTACAGAAGAACCCG
TTGTCATTTGCAATGACAGACACCGTTTCTTAGTCGCTGAACAACTCCGTGAAATTGACAAGTTAGCAAATAATATTATT
CTCGAACCGGTAGGCCGTAATACTGCACCAGCGATCGCTCTTGCCGCGTTTTGTGCGCTCCAGAATGCTGATAATGCTGA
TCCTCTTTTGTTGGTTCTTGCTGCAGATCATGTGATTCAGGATGAAATAGCTTTTACGAAAGCTGTCAGACATGCTGAAG
AATACGCTGCAAATGGTAAGCTTGTAACTTTTGGTATTGTTCCAACGCATGCTGAAACGGGTTATGGATATATTCGTCGT
GGTGAGTTGATAGGAAATGACGCTTATGCAGTGGCTGAATTTGTGGAGAAACCGGATATCGATACCGCCGGTGACTATTT
CAAATCAGGGAAATATTACTGGAATAGCGGTATGTTTTTATTTCGTGCAAGCTCTTATTTAAACGAATTAAAGTATTTAT
CACCTGAAATTTATAAAGCTTGTGAAAAGGCGGTAGGACATATAAATCCCGATCTTGATTTTATTCGTATTGATAAAGAA
GAGTTTATGTCATGCCCGAGTGATTCTATCGATTATGCAGTTATGGAGCACACACAGCATGCGGTGGTGATACCAATGAG
CGCTGGCTGGTCGGATGTGGGTTCCTGGTCCTCACTTTGGGATATATCGAATAAAGATCATCAGAGAAATGTTTTAAAAG
GAGATATTTTCGCACATGCTTGTAATGATAATTACATTTATTCCGAAGATATGTTTATAAGTGCGATTGGTGTAAGCAAT
CTTGTCATTGTTCAAACAACAGACGCTTTACTGGTGGCTAATAAAGATACAGTACAAGATGTTAAAAAAATTGTCGATTA
TTTAAAACGGAATGATAGGAACGAATATAAACAACATCAAGAAGTTTTCCGCCCCTGGGGAAAATATAATGTGATTGATA
GCGGCAAAAATTACCTCGTTCGATGTATCACTGTTAAGCCGGGTGAGAAATTTGTGGCGCAGATGCATCACCACCGGGCT
GAGCATTGGATAGTATTATCCGGGACTGCTCGTGTTACAAAGGGAGAGCAGACTTATATGGTTTCTGAAAATGAATCAAC
ATTTATTCCTCCGAATACTATTCACGCGCTGGAAAATCCTGGAATGACCCCCCTGAAGTTAATTGAGATTCAATCAGGTA
CCTATCTTGGTGAGGATGATATTATTCGTTTAGAACAACGTTCTGGATTTTCGAAGGAGTGGACTAATGAACGTAGTTAA

Upstream 100 bases:

>100_bases
GCATTGGCAATCTTTACCGTTGTCTACATGTCGCTATTTTAGCATGTACAAGAGTTATTGGAATAATATCCAATATTCTT
CGTCAAAAGAGATAAAATAA

Downstream 100 bases:

>100_bases
TAATAGCCGTGATGTTATTTATTCATCAGGTATTGTGTTTGGAACGAGTGGGGCTCGCGGTCTTGTAAAAGATTTTACAC
CTCAGGTATGTGCTGCTTTT

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP; GTP--mannose-1-phosphate guanylyltransferase

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDRHRFLVAEQLREIDKLANNII
LEPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQDEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRR
GELIGNDAYAVAEFVEKPDIDTAGDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKE
EFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHACNDNYIYSEDMFISAIGVSN
LVIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQEVFRPWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRA
EHWIVLSGTARVTKGEQTYMVSENESTFIPPNTIHALENPGMTPLKLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS

Sequences:

>Translated_479_residues
MSFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDRHRFLVAEQLREIDKLANNII
LEPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQDEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRR
GELIGNDAYAVAEFVEKPDIDTAGDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKE
EFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHACNDNYIYSEDMFISAIGVSN
LVIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQEVFRPWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRA
EHWIVLSGTARVTKGEQTYMVSENESTFIPPNTIHALENPGMTPLKLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS
>Mature_478_residues
SFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDRHRFLVAEQLREIDKLANNIIL
EPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQDEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRRG
ELIGNDAYAVAEFVEKPDIDTAGDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKEE
FMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHACNDNYIYSEDMFISAIGVSNL
VIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQEVFRPWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRAE
HWIVLSGTARVTKGEQTYMVSENESTFIPPNTIHALENPGMTPLKLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family

Homologues:

Organism=Escherichia coli, GI1788362, Length=471, Percent_Identity=59.0233545647558, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFBM_SALTY (P26404)

Other databases:

- EMBL:   X56793
- EMBL:   AE006468
- PIR:   S15312
- RefSeq:   NP_461029.1
- ProteinModelPortal:   P26404
- SMR:   P26404
- PRIDE:   P26404
- GeneID:   1253605
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2084
- NMPDR:   fig|99287.1.peg.2009
- HOGENOM:   HBG422488
- OMA:   AILMEPF
- ProtClustDB:   CLSK894266
- BioCyc:   STYP99287:STM2084-MONOMER
- BRENDA:   2.7.7.13
- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- TIGRFAMs:   TIGR01479

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase; SSF51182 RmlC_like_cupin

EC number: =2.7.7.13

Molecular weight: Translated: 54047; Mature: 53916

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDR
CCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
HRFLVAEQLREIDKLANNIILEPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQ
CCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHH
DEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRRGELIGNDAYAVAEFVEKPDI
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECEEECCCHHHHHHHHHCCCC
DTAGDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKE
CCCHHHHHCCCEEEECCEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECHH
EFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHA
HHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCCHHCCCCCCCEEEEE
CNDNYIYSEDMFISAIGVSNLVIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQ
CCCCEEECCCCEEEEECCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHH
EVFRPWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRAEHWIVLSGTARVTKGEQTYM
HHHCCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCEEEECCCEEEE
VSENESTFIPPNTIHALENPGMTPLKLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS
EECCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCEECCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRLASLSTEEPVVICNDR
CCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
HRFLVAEQLREIDKLANNIILEPVGRNTAPAIALAAFCALQNADNADPLLLVLAADHVIQ
CCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHH
DEIAFTKAVRHAEEYAANGKLVTFGIVPTHAETGYGYIRRGELIGNDAYAVAEFVEKPDI
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECEEECCCHHHHHHHHHCCCC
DTAGDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKE
CCCHHHHHCCCEEEECCEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECHH
EFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHA
HHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCCHHCCCCCCCEEEEE
CNDNYIYSEDMFISAIGVSNLVIVQTTDALLVANKDTVQDVKKIVDYLKRNDRNEYKQHQ
CCCCEEECCCCEEEEECCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHH
EVFRPWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHRAEHWIVLSGTARVTKGEQTYM
HHHCCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCEEEECCCEEEE
VSENESTFIPPNTIHALENPGMTPLKLIEIQSGTYLGEDDIIRLEQRSGFSKEWTNERS
EECCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCEECCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609