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The map label for this gene is rfbK

Identifier: 16765413

GI number: 16765413

Start: 2162433

End: 2163866

Strand: Reverse

Name: rfbK

Synonym: STM2083

Alternate gene names: 16765413

Gene position: 2163866-2162433 (Counterclockwise)

Preceding gene: 16765414

Following gene: 16765412

Centisome position: 44.55

GC content: 40.86

Gene sequence:

>1434_bases
ATGAACGTAGTTAATAATAGCCGTGATGTTATTTATTCATCAGGTATTGTGTTTGGAACGAGTGGGGCTCGCGGTCTTGT
AAAAGATTTTACACCTCAGGTATGTGCTGCTTTTACGGTTTCATTTGTTGCCGTTATGCAGGAACATTTTTCCTTTGATA
CCGTAGCATTGGCAATAGATAATCGTCCAAGTAGTTATGGGATGGCTCAGGCGTGTGCTGCTGCATTGGCGGATAAAGGC
GTTAACTGTATTTTTTATGGAGTGGTACCAACCCCAGCTTTGGCCTTTCAGTCTATGTCTGACAATATGCCTGCGATAAT
GGTTACGGGAAGTCATATTCCATTCGAGCGGAACGGCCTCAAGTTTTATCGTCCTGATGGTGAAATCACGAAACATGATG
AGGCTGCGATCCTTAGTGTTGAAGATACGTGCAGCCATTTAGAGCTTAAAGAACTCATAGTTTCAGAAATGGCTGCTGTT
AATTATATATCTCGTTATACATCTTTATTTTCTACTCCATTCCTGAAAAATAAGCGTATTGGTATTTACGAACATTCAAG
CGCTGGGCGTGATCTTTATAAGCCTTTATTTATTGCATTGGGGGCTGAAGTCGTTAGCTTGGGTAGAAGCGATAATTTTG
TACCTATAGATACAGAGGCTGTAAGCAAAGAGGATCGGGAAAAAGCTCGCTCATGGGCTAAAGAGTTCGATTTAGATGCC
ATATTCTCGACAGATGGGGATGGTGATCGCCCTCTTATTGCTGATGAGGCCGGTGAGTGGCTAAGAGGCGATATACTAGG
TCTATTATGTTCACTTGCATTGGATGCAGAAGCCGTCGCTATTCCTGTTAGTTGTAACAGCATAATTTCTTCTGGCCGCT
TTTTTAAACATGTTAAGCTTACAAAAATTGGCTCGCCTTATGTTATCGAAGCTTTTAATGAATTATCGCGGAGTTATAGT
CGTATTGTCGGTTTTGAAGCCAATGGCGGTTTTTTATTAGGAAGCGACATCTGTATTAACGAGCAGAATCTTCATGCCTT
ACCAACTCGTGATGCTGTATTACCAGCAATAATGCTGCTTTACAAAAGTAGGAATACCAGCATTAGCGCTTTAGTCAATG
AACTCCCAACTCGTTACACCCATTCTGACAGATTACAGGGGATTACAACTGATAAAAGTCAATCCTTAATTAGTATGGGC
AGAGAAAATCTGAGCAACCTCTTAAGCTATATTGGTTTGGAGAATGAAGGTGCAATTTCTACAGATATGACAGATGGTAT
GCGAATTACTTTACGTGATGGATGTATTGTGCATTTGCGCGCTTCTGGTAATGCACCTGAGTTACGCTGCTATGCAGAAG
CTAATTTATTAAATAGGGCTCAGGATCTTGTAAATACAACGCTTGCTAATATTAAAAAACGATGCTTGCTGTAA

Upstream 100 bases:

>100_bases
ACCCCCCTGAAGTTAATTGAGATTCAATCAGGTACCTATCTTGGTGAGGATGATATTATTCGTTTAGAACAACGTTCTGG
ATTTTCGAAGGAGTGGACTA

Downstream 100 bases:

>100_bases
AAAAATTGAATGTTATTTACTTAATATGCCTATTTTATTTACATTATGCACGGTCAGAGGGTGAGGATTAAATGGATAAT
ATTGATAATAAGTATAATCC

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNVVNNSRDVIYSSGIVFGTSGARGLVKDFTPQVCAAFTVSFVAVMQEHFSFDTVALAIDNRPSSYGMAQACAAALADKG
VNCIFYGVVPTPALAFQSMSDNMPAIMVTGSHIPFERNGLKFYRPDGEITKHDEAAILSVEDTCSHLELKELIVSEMAAV
NYISRYTSLFSTPFLKNKRIGIYEHSSAGRDLYKPLFIALGAEVVSLGRSDNFVPIDTEAVSKEDREKARSWAKEFDLDA
IFSTDGDGDRPLIADEAGEWLRGDILGLLCSLALDAEAVAIPVSCNSIISSGRFFKHVKLTKIGSPYVIEAFNELSRSYS
RIVGFEANGGFLLGSDICINEQNLHALPTRDAVLPAIMLLYKSRNTSISALVNELPTRYTHSDRLQGITTDKSQSLISMG
RENLSNLLSYIGLENEGAISTDMTDGMRITLRDGCIVHLRASGNAPELRCYAEANLLNRAQDLVNTTLANIKKRCLL

Sequences:

>Translated_477_residues
MNVVNNSRDVIYSSGIVFGTSGARGLVKDFTPQVCAAFTVSFVAVMQEHFSFDTVALAIDNRPSSYGMAQACAAALADKG
VNCIFYGVVPTPALAFQSMSDNMPAIMVTGSHIPFERNGLKFYRPDGEITKHDEAAILSVEDTCSHLELKELIVSEMAAV
NYISRYTSLFSTPFLKNKRIGIYEHSSAGRDLYKPLFIALGAEVVSLGRSDNFVPIDTEAVSKEDREKARSWAKEFDLDA
IFSTDGDGDRPLIADEAGEWLRGDILGLLCSLALDAEAVAIPVSCNSIISSGRFFKHVKLTKIGSPYVIEAFNELSRSYS
RIVGFEANGGFLLGSDICINEQNLHALPTRDAVLPAIMLLYKSRNTSISALVNELPTRYTHSDRLQGITTDKSQSLISMG
RENLSNLLSYIGLENEGAISTDMTDGMRITLRDGCIVHLRASGNAPELRCYAEANLLNRAQDLVNTTLANIKKRCLL
>Mature_477_residues
MNVVNNSRDVIYSSGIVFGTSGARGLVKDFTPQVCAAFTVSFVAVMQEHFSFDTVALAIDNRPSSYGMAQACAAALADKG
VNCIFYGVVPTPALAFQSMSDNMPAIMVTGSHIPFERNGLKFYRPDGEITKHDEAAILSVEDTCSHLELKELIVSEMAAV
NYISRYTSLFSTPFLKNKRIGIYEHSSAGRDLYKPLFIALGAEVVSLGRSDNFVPIDTEAVSKEDREKARSWAKEFDLDA
IFSTDGDGDRPLIADEAGEWLRGDILGLLCSLALDAEAVAIPVSCNSIISSGRFFKHVKLTKIGSPYVIEAFNELSRSYS
RIVGFEANGGFLLGSDICINEQNLHALPTRDAVLPAIMLLYKSRNTSISALVNELPTRYTHSDRLQGITTDKSQSLISMG
RENLSNLLSYIGLENEGAISTDMTDGMRITLRDGCIVHLRASGNAPELRCYAEANLLNRAQDLVNTTLANIKKRCLL

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family

Homologues:

Organism=Escherichia coli, GI1789566, Length=257, Percent_Identity=31.5175097276265, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFBK_SALTY (P26405)

Other databases:

- EMBL:   X56793
- EMBL:   AE006468
- PIR:   S15313
- RefSeq:   NP_461028.1
- ProteinModelPortal:   P26405
- PRIDE:   P26405
- GeneID:   1253604
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2083
- NMPDR:   fig|99287.1.peg.2008
- HOGENOM:   HBG644964
- OMA:   RCYAEAD
- ProtClustDB:   CLSK894265
- BioCyc:   STYP99287:STM2083-MONOMER
- BRENDA:   5.4.2.8
- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- Gene3D:   G3DSA:3.40.120.10

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV; SSF53738 A-D-PHexomutase_a/b/a-I/II/III

EC number: =5.4.2.8

Molecular weight: Translated: 52087; Mature: 52087

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00710 PGM_PMM

Important sites: ACT_SITE 111-111

Signals:

None

Transmembrane regions:

HASH(0xedcb4f8)-; HASH(0xb4735ec)-;

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVNNSRDVIYSSGIVFGTSGARGLVKDFTPQVCAAFTVSFVAVMQEHFSFDTVALAID
CCCCCCCCCEEEECCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
NRPSSYGMAQACAAALADKGVNCIFYGVVPTPALAFQSMSDNMPAIMVTGSHIPFERNGL
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCC
KFYRPDGEITKHDEAAILSVEDTCSHLELKELIVSEMAAVNYISRYTSLFSTPFLKNKRI
EEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GIYEHSSAGRDLYKPLFIALGAEVVSLGRSDNFVPIDTEAVSKEDREKARSWAKEFDLDA
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHCHHHHHHHHHHHHHCCCCE
IFSTDGDGDRPLIADEAGEWLRGDILGLLCSLALDAEAVAIPVSCNSIISSGRFFKHVKL
EEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCEEEEEEE
TKIGSPYVIEAFNELSRSYSRIVGFEANGGFLLGSDICINEQNLHALPTRDAVLPAIMLL
EECCCCHHHHHHHHHHHHHHHHEEEECCCCEEECCCEEECCCCCCCCCCCHHHHHHHHHH
YKSRNTSISALVNELPTRYTHSDRLQGITTDKSQSLISMGRENLSNLLSYIGLENEGAIS
HHCCCCHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TDMTDGMRITLRDGCIVHLRASGNAPELRCYAEANLLNRAQDLVNTTLANIKKRCLL
CCCCCCEEEEEECCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MNVVNNSRDVIYSSGIVFGTSGARGLVKDFTPQVCAAFTVSFVAVMQEHFSFDTVALAID
CCCCCCCCCEEEECCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
NRPSSYGMAQACAAALADKGVNCIFYGVVPTPALAFQSMSDNMPAIMVTGSHIPFERNGL
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCC
KFYRPDGEITKHDEAAILSVEDTCSHLELKELIVSEMAAVNYISRYTSLFSTPFLKNKRI
EEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GIYEHSSAGRDLYKPLFIALGAEVVSLGRSDNFVPIDTEAVSKEDREKARSWAKEFDLDA
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHCHHHHHHHHHHHHHCCCCE
IFSTDGDGDRPLIADEAGEWLRGDILGLLCSLALDAEAVAIPVSCNSIISSGRFFKHVKL
EEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCEEEEEEE
TKIGSPYVIEAFNELSRSYSRIVGFEANGGFLLGSDICINEQNLHALPTRDAVLPAIMLL
EECCCCHHHHHHHHHHHHHHHHEEEECCCCEEECCCEEECCCCCCCCCCCHHHHHHHHHH
YKSRNTSISALVNELPTRYTHSDRLQGITTDKSQSLISMGRENLSNLLSYIGLENEGAIS
HHCCCCHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TDMTDGMRITLRDGCIVHLRASGNAPELRCYAEANLLNRAQDLVNTTLANIKKRCLL
CCCCCCEEEEEECCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609