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The map label for this gene is pduQ [H]

Identifier: 16765382

GI number: 16765382

Start: 2127627

End: 2128739

Strand: Direct

Name: pduQ [H]

Synonym: STM2052

Alternate gene names: 16765382

Gene position: 2127627-2128739 (Clockwise)

Preceding gene: 16765381

Following gene: 16765383

Centisome position: 43.8

GC content: 60.38

Gene sequence:

>1113_bases
ATGAATACCTTCTCACTACAAACGCGGTTGTACAGCGGTCAGGGCAGCCTGGCGGTGCTCAAGCGCTTTACCAATAAGCA
CATCTGGATAATCTGCGATGGCTTTCTGGCGCACTCTCCGCTGCTGGATACCCTGCGTAACGCGCTGCCCGCAGATAACC
GCATCAGCGTCTTTAGCGAGATAACGCCGGACCCCACCATCCACACAGTGGTTCAGGGCATTGCGCAAATGCAGGCTCTG
CAACCGCAGGTGGTGATCGGTTTTGGCGGCGGCTCGGCAATGGACGCGGCGAAAGCGATTGTCTGGTTTAGCCAGCAGAG
CGGGATCAACATCGAAACCTGCGTGGCGATCCCGACCACCAGCGGCACCGGCTCGGAAGTGACCAGCGCCTGCGTAATTA
GCGACCCGGATAAAGGCATCAAGTATCCGCTGTTCAACAATGCGCTGTATCCGGATATGGCGATCCTCGACCCGGAGCTG
GTGGTCAGCGTACCGCCGCAGATTACCGCCAACACCGGCATGGACGTGCTGACCCACGCCCTGGAGGCCTGGGTGTCACC
GCGCGCCAGCGACTTTACCGACGCGCTAGCGGAAAAAGCCGCCAAACTGGTGTTCCAGTATCTGCCCACGGCGGTGGAAA
AAGGCGACTGCGTGGCGACGCGCGGGAAAATGCACAATGCCTCAACGCTCGCCGGGATGGCCTTCAGCCAGGCGGGGCTG
GGGCTTAACCACGCGATAGCCCACCAGCTTGGCGGGCAGTTTCATCTGCCGCATGGCCTGGCCAACGCCCTGCTGTTAAC
CACGGTTATCCGTTTTAACGCCGGCGTTCCGCGCGCCGCCAAACGCTACGCGCGGCTGGCCAAAGCCTGCGGTTTTTGCC
CGGCAGAAGCCAATGATATCGCGGCGATCAATGCGCTGATTCAGCAAATCGAACTGCTTAAGCAACGCTGTGTCCTTCCC
TCACTGGCCGTTGCGCTTAAAGAAGGAAGATCCGACTTTTCCGCACGCATTCCGGCGATGGTGCAGGCTGCGCTGGCTGA
CGTTACGCTGCGCACTAACCCGCGCCCGGCCAATGCCGAAGCCATTCGGGAACTGCTGGAGGAACTGCTATGA

Upstream 100 bases:

>100_bases
CCACACCAACCGGTGAAGGGACCACGTCAGCGCGTACTTTTGCCCGTTCCCGGCGCTGCGTACTGACCAACGGCTTTTCT
ATTCGCTAACTGAGGTCGCG

Downstream 100 bases:

>100_bases
GCACCGCCATCAACAGCGTTGAAATGTCCCTTAGCGCCGATGAAATTCGCGAGCGCGTTCGCGCAGCGGGCGTTGTCGGC
GCGGGCGGCGCAGGTTTTCC

Product: propanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLAHSPLLDTLRNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPRASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDIAAINALIQQIELLKQRCVLP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAEAIRELLEELL

Sequences:

>Translated_370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLAHSPLLDTLRNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPRASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDIAAINALIQQIELLKQRCVLP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAEAIRELLEELL
>Mature_370_residues
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLAHSPLLDTLRNALPADNRISVFSEITPDPTIHTVVQGIAQMQAL
QPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPEL
VVSVPPQITANTGMDVLTHALEAWVSPRASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDIAAINALIQQIELLKQRCVLP
SLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAEAIRELLEELL

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=327, Percent_Identity=26.2996941896024, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1787493, Length=405, Percent_Identity=36.0493827160494, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI87082107, Length=382, Percent_Identity=38.7434554973822, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI48994951, Length=348, Percent_Identity=36.7816091954023, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789163, Length=383, Percent_Identity=32.3759791122715, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789386, Length=344, Percent_Identity=26.1627906976744, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=401, Percent_Identity=25.1870324189526, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6321181, Length=375, Percent_Identity=32, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24657991, Length=410, Percent_Identity=24.8780487804878, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 39470; Mature: 39470

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLAHSPLLDTLRNALPADNRISVFSE
CCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEHHHC
ITPDPTIHTVVQGIAQMQALQPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTT
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEEEEECC
SGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPELVVSVPPQITANTGMDVLTHA
CCCCHHHHCEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHEEECCCCCCCCCCHHHHHHH
LEAWVSPRASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDI
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHH
AAINALIQQIELLKQRCVLPSLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAE
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCCCHH
AIRELLEELL
HHHHHHHHHC
>Mature Secondary Structure
MNTFSLQTRLYSGQGSLAVLKRFTNKHIWIICDGFLAHSPLLDTLRNALPADNRISVFSE
CCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEHHHC
ITPDPTIHTVVQGIAQMQALQPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETCVAIPTT
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHEEEECCCCCCEEEEEEEECC
SGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPELVVSVPPQITANTGMDVLTHA
CCCCHHHHCEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHEEECCCCCCCCCCHHHHHHH
LEAWVSPRASDFTDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNASTLAGMAFSQAGL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCC
GLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAKRYARLAKACGFCPAEANDI
CHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHH
AAINALIQQIELLKQRCVLPSLAVALKEGRSDFSARIPAMVQAALADVTLRTNPRPANAE
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCCCHH
AIRELLEELL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]