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The map label for this gene is yhjG [H]

Identifier: 16764891

GI number: 16764891

Start: 1622640

End: 1624148

Strand: Reverse

Name: yhjG [H]

Synonym: STM1546

Alternate gene names: 16764891

Gene position: 1624148-1622640 (Counterclockwise)

Preceding gene: 16764894

Following gene: 16764890

Centisome position: 33.44

GC content: 52.88

Gene sequence:

>1509_bases
ATGACAAGATACACCACTACAGATGTACTGATCTGCGGCGCTGGCGTTACCGGGCTTACTTTAGCCATCGAACTGGCGCG
TCACGGGGTCTCATTTCGCCTGATTGAGAAAAGAACGACGCCTTTTATCGGCTCCCGCGGTAAAGGTATTCAGCCGCGAA
CGCAAGAAATCTTCGAGGATCTTGGAATACTCAATAAAGTGGTCGCCGCCGGCGGGCTCTATCCCCGACTACGAACCTAC
CGTCACGACGGAAGCTATGTAGATTCAGATATTGCGCATCATACAAAACCGACCCACGCCGAACCCTACCATCTGCCATT
GATGGTTCCACAGAACGTGACAGAAACGATTATGCGAGAGCAATTAAAAGCATGGGGACATCGGGTGGAGTTCGGCTGCG
AGTTACGACATTTTGCGCAAACCCCACGCACAGTCACCGCGTATGTTGCCGGGCCGGCTGGAGAAGAGGTCATCATCGCG
CATTATTTGATAGGTGCGGATGGCGGCGGAAGCTTTGTCAGAAAAAAATTAGGCGTCAGCTTCCCCGGTCGTACGTTGGG
AATCCATGCGCTTGTGGCTGACGCTTCACTTTCCGGTCTGAACCGGGATGTATGGCACCATTTTAACGATGGCGATATGG
CGCGGATGATAACGATTTGCCCTCTCGCCGGCACGCAGCTCTTTCAAATCCAGGCGTTGTTGGCCCCGGACGACTCGCAG
AATTTCTCAGCCGACGTGCTCACGGCTTTTCTTACTGAACGAATCGGGCGGACGGATGTTCGCATCCATTCGATTCCCTG
GGTATCGAAGTACCAGATGAATGCGCGCATAGCGGAACATTACCGTGTGGGTAAGGTATTTTTAGCCGGTGATGCCGCTC
ATGTGCATCCGCCGACGGGTGGGCAAGGGTTAAATACCAGCATCCAGGATGCCTATAACCTGGGATGGAAAATGGCAGCC
TCGCTGCGGGGCGCAGGAGAAGAACTTCTCGATAGCTATGAACAGGAACGCCGCCCGATAGCCGAATCACTGCTCCACCT
TTCTACCCGATTACTTGACTCTCAAAAACAGAGAGGCATCAAACGAGAACGCGATGTTCAACAACTTGACATCCAGTATA
CCAACTCCCCCTTAGCCCACACGTTACCCGAACGTCAGCATGGGTTACAAGCGGGAGAAAGAGCTCCGGATGCTCCTCTT
TTGGGCGCCGGCGGCCAATCATTACGATTATTTCAGTTACTACAAGGTCCGGACTGGAATCTGTTGGCCTACGAAACCCA
TGGTAAGGTCATCGATGCGCGGCGAGGACTGCGTATTCATCACATTGGCGAGCAGGACGAGCTCATCGATACCCTGGGGC
ACTTCCGGGAATCCTATCATCTGGCGCCGGGTCAATGCGTGCTGATCCGACCCGATGGCTATGTCGGCGCATTCTTTCAC
GGCAAACAGAGCAACGATATTGAAAATTATCTCTCTCGTTTTGCCATAGGGATTAAAGATGAATACTAA

Upstream 100 bases:

>100_bases
AGCGCCTTCATTTGCTCGGAAATATGTGTATCTTGCACATTTTTATTTTCTGACATAGCATGTCTCCTCTTATGTGCATA
ATACACATATCGGAGAGACG

Downstream 100 bases:

>100_bases
TGTCTATGAGAACACCGACAGCGAAACTATCACCCCGCTCAACAAGCGGCGTATTTTGCCTGTTTTCCTGCTTGTCGGCC
TTTACGCCGCCAGTACCGCG

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTRYTTTDVLICGAGVTGLTLAIELARHGVSFRLIEKRTTPFIGSRGKGIQPRTQEIFEDLGILNKVVAAGGLYPRLRTY
RHDGSYVDSDIAHHTKPTHAEPYHLPLMVPQNVTETIMREQLKAWGHRVEFGCELRHFAQTPRTVTAYVAGPAGEEVIIA
HYLIGADGGGSFVRKKLGVSFPGRTLGIHALVADASLSGLNRDVWHHFNDGDMARMITICPLAGTQLFQIQALLAPDDSQ
NFSADVLTAFLTERIGRTDVRIHSIPWVSKYQMNARIAEHYRVGKVFLAGDAAHVHPPTGGQGLNTSIQDAYNLGWKMAA
SLRGAGEELLDSYEQERRPIAESLLHLSTRLLDSQKQRGIKRERDVQQLDIQYTNSPLAHTLPERQHGLQAGERAPDAPL
LGAGGQSLRLFQLLQGPDWNLLAYETHGKVIDARRGLRIHHIGEQDELIDTLGHFRESYHLAPGQCVLIRPDGYVGAFFH
GKQSNDIENYLSRFAIGIKDEY

Sequences:

>Translated_502_residues
MTRYTTTDVLICGAGVTGLTLAIELARHGVSFRLIEKRTTPFIGSRGKGIQPRTQEIFEDLGILNKVVAAGGLYPRLRTY
RHDGSYVDSDIAHHTKPTHAEPYHLPLMVPQNVTETIMREQLKAWGHRVEFGCELRHFAQTPRTVTAYVAGPAGEEVIIA
HYLIGADGGGSFVRKKLGVSFPGRTLGIHALVADASLSGLNRDVWHHFNDGDMARMITICPLAGTQLFQIQALLAPDDSQ
NFSADVLTAFLTERIGRTDVRIHSIPWVSKYQMNARIAEHYRVGKVFLAGDAAHVHPPTGGQGLNTSIQDAYNLGWKMAA
SLRGAGEELLDSYEQERRPIAESLLHLSTRLLDSQKQRGIKRERDVQQLDIQYTNSPLAHTLPERQHGLQAGERAPDAPL
LGAGGQSLRLFQLLQGPDWNLLAYETHGKVIDARRGLRIHHIGEQDELIDTLGHFRESYHLAPGQCVLIRPDGYVGAFFH
GKQSNDIENYLSRFAIGIKDEY
>Mature_501_residues
TRYTTTDVLICGAGVTGLTLAIELARHGVSFRLIEKRTTPFIGSRGKGIQPRTQEIFEDLGILNKVVAAGGLYPRLRTYR
HDGSYVDSDIAHHTKPTHAEPYHLPLMVPQNVTETIMREQLKAWGHRVEFGCELRHFAQTPRTVTAYVAGPAGEEVIIAH
YLIGADGGGSFVRKKLGVSFPGRTLGIHALVADASLSGLNRDVWHHFNDGDMARMITICPLAGTQLFQIQALLAPDDSQN
FSADVLTAFLTERIGRTDVRIHSIPWVSKYQMNARIAEHYRVGKVFLAGDAAHVHPPTGGQGLNTSIQDAYNLGWKMAAS
LRGAGEELLDSYEQERRPIAESLLHLSTRLLDSQKQRGIKRERDVQQLDIQYTNSPLAHTLPERQHGLQAGERAPDAPLL
GAGGQSLRLFQLLQGPDWNLLAYETHGKVIDARRGLRIHHIGEQDELIDTLGHFRESYHLAPGQCVLIRPDGYVGAFFHG
KQSNDIENYLSRFAIGIKDEY

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=350, Percent_Identity=28.8571428571429, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 55740; Mature: 55609

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYTTTDVLICGAGVTGLTLAIELARHGVSFRLIEKRTTPFIGSRGKGIQPRTQEIFED
CCCEEECCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LGILNKVVAAGGLYPRLRTYRHDGSYVDSDIAHHTKPTHAEPYHLPLMVPQNVTETIMRE
HHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHH
QLKAWGHRVEFGCELRHFAQTPRTVTAYVAGPAGEEVIIAHYLIGADGGGSFVRKKLGVS
HHHHCCCEEECCHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHCCC
FPGRTLGIHALVADASLSGLNRDVWHHFNDGDMARMITICPLAGTQLFQIQALLAPDDSQ
CCCCHHEEEEEEECCHHCCCCHHHHCCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCC
NFSADVLTAFLTERIGRTDVRIHSIPWVSKYQMNARIAEHYRVGKVFLAGDAAHVHPPTG
CCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHEECEEEEECCCCEECCCCC
GQGLNTSIQDAYNLGWKMAASLRGAGEELLDSYEQERRPIAESLLHLSTRLLDSQKQRGI
CCCCCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
KRERDVQQLDIQYTNSPLAHTLPERQHGLQAGERAPDAPLLGAGGQSLRLFQLLQGPDWN
HHHCCHHHEEEEECCCCHHHCCCCHHHCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCC
LLAYETHGKVIDARRGLRIHHIGEQDELIDTLGHFRESYHLAPGQCVLIRPDGYVGAFFH
EEEEECCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEE
GKQSNDIENYLSRFAIGIKDEY
CCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TRYTTTDVLICGAGVTGLTLAIELARHGVSFRLIEKRTTPFIGSRGKGIQPRTQEIFED
CCEEECCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LGILNKVVAAGGLYPRLRTYRHDGSYVDSDIAHHTKPTHAEPYHLPLMVPQNVTETIMRE
HHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHH
QLKAWGHRVEFGCELRHFAQTPRTVTAYVAGPAGEEVIIAHYLIGADGGGSFVRKKLGVS
HHHHCCCEEECCHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHCCC
FPGRTLGIHALVADASLSGLNRDVWHHFNDGDMARMITICPLAGTQLFQIQALLAPDDSQ
CCCCHHEEEEEEECCHHCCCCHHHHCCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCC
NFSADVLTAFLTERIGRTDVRIHSIPWVSKYQMNARIAEHYRVGKVFLAGDAAHVHPPTG
CCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHEECEEEEECCCCEECCCCC
GQGLNTSIQDAYNLGWKMAASLRGAGEELLDSYEQERRPIAESLLHLSTRLLDSQKQRGI
CCCCCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
KRERDVQQLDIQYTNSPLAHTLPERQHGLQAGERAPDAPLLGAGGQSLRLFQLLQGPDWN
HHHCCHHHEEEEECCCCHHHCCCCHHHCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCC
LLAYETHGKVIDARRGLRIHHIGEQDELIDTLGHFRESYHLAPGQCVLIRPDGYVGAFFH
EEEEECCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEE
GKQSNDIENYLSRFAIGIKDEY
CCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]