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The map label for this gene is pflE [H]

Identifier: 16764206

GI number: 16764206

Start: 913785

End: 914684

Strand: Reverse

Name: pflE [H]

Synonym: STM0844

Alternate gene names: 16764206

Gene position: 914684-913785 (Counterclockwise)

Preceding gene: 16764207

Following gene: 16764205

Centisome position: 18.83

GC content: 55.56

Gene sequence:

>900_bases
ATGATCTTCAATATTCAGCGCTATTCTACCCACGATGGCCCCGGTATCCGTACCGTAGTATTTCTCAAAGGCTGTTCGTT
GGGCTGTCGCTGGTGCCAGAACCCGGAAAGCCGCGCCCGCGCTCAGGATCTGTTATACGACGCGCGGCTGTGTCTCGAAG
GATGCGACCTGTGCGCGCAAGCCGCGCCGGACGTCATTGAGCGCGCGCTAAACGGCCTGCTCATCCATCGCGAGAAACTG
ACCGACGCCCATTTTTCCATCCTGGCGCATTGCTGCCCCACACAGGCGTTAACCGTGTGCGGCGAAATAAAAAGCGTGGA
TGAGATCATGGCGACGGTACTGCGCGATAAACCTTTTTACGATCGCAGCGGCGGCGGTCTTACGCTTTCCGGCGGCGAAC
CGTTTATGCAGCCGGAACTGGCGGCAGAGCTGTTTAAAGCCAGCCATGACGCCGGTATTCATACTGCGGTTGAGACCTGT
CTGCATGTCCCATGGAAATACATTGCGCCCTCACTGCCTTATATCGATCTGTTTCTGGCTGATTTGAAGCATGTCGCCGA
CGGGCCGTTTAAGCAGTGGACCGATGGCAGCGCCTCGCGGGTGCTGGAAAATCTCAGGAAACTCGCCGCCGCGGGCAAAA
AAATGGTGATTCGCGTCCCGCTGATTCAGGGATTTAATGCCGATGAGGAGGCCATTAAAGCCATTACCGACTTTGCCGCC
GACGAACTGCACGTCGGGGAAATTCATTTTCTGCCCTACCACACGCTGGGCATCAATAAATACCACTTACTCAGTCAACC
CTATCATGCCCCGGATAAACCACTGGATGCGCCGGCGTTACTTGATTTTGCCCAGAAGTACGCCTGCCAAAAAGGTTTAA
CCGCGACCCTACGAGGATAA

Upstream 100 bases:

>100_bases
TAACGCCTCTTTGTCAGAACCTCTCCATTCGTTGACGCACATCAAGATAGCTTTCATTCGAAAGTAATTTAATCTTTATA
TGAAATAAGAGAGGCCGTTT

Downstream 100 bases:

>100_bases
TTATCATGACCCAACTGAAACTGGACACGCTCAGTGACCGCATTAAGGCGCACAAAACCGCGCTGGTACATATCGTGAAA
CCGCCGGTCTGTACCGAGCG

Product: pyruvate formate lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARAQDLLYDARLCLEGCDLCAQAAPDVIERALNGLLIHREKL
TDAHFSILAHCCPTQALTVCGEIKSVDEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPELAAELFKASHDAGIHTAVETC
LHVPWKYIAPSLPYIDLFLADLKHVADGPFKQWTDGSASRVLENLRKLAAAGKKMVIRVPLIQGFNADEEAIKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLSQPYHAPDKPLDAPALLDFAQKYACQKGLTATLRG

Sequences:

>Translated_299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARAQDLLYDARLCLEGCDLCAQAAPDVIERALNGLLIHREKL
TDAHFSILAHCCPTQALTVCGEIKSVDEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPELAAELFKASHDAGIHTAVETC
LHVPWKYIAPSLPYIDLFLADLKHVADGPFKQWTDGSASRVLENLRKLAAAGKKMVIRVPLIQGFNADEEAIKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLSQPYHAPDKPLDAPALLDFAQKYACQKGLTATLRG
>Mature_299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARAQDLLYDARLCLEGCDLCAQAAPDVIERALNGLLIHREKL
TDAHFSILAHCCPTQALTVCGEIKSVDEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPELAAELFKASHDAGIHTAVETC
LHVPWKYIAPSLPYIDLFLADLKHVADGPFKQWTDGSASRVLENLRKLAAAGKKMVIRVPLIQGFNADEEAIKAITDFAA
DELHVGEIHFLPYHTLGINKYHLLSQPYHAPDKPLDAPALLDFAQKYACQKGLTATLRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=87.9598662207358, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1790389, Length=299, Percent_Identity=34.4481605351171, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1787130, Length=270, Percent_Identity=26.6666666666667, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790839, Length=233, Percent_Identity=26.6094420600858, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 32949; Mature: 32949

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARAQDLLYDARLCLEGCDLCAQ
CEEECEECCCCCCCCCEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAPDVIERALNGLLIHREKLTDAHFSILAHCCPTQALTVCGEIKSVDEIMATVLRDKPFY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRSGGGLTLSGGEPFMQPELAAELFKASHDAGIHTAVETCLHVPWKYIAPSLPYIDLFLA
CCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCCHHHHHHH
DLKHVADGPFKQWTDGSASRVLENLRKLAAAGKKMVIRVPLIQGFNADEEAIKAITDFAA
HHHHHCCCCCHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
DELHVGEIHFLPYHTLGINKYHLLSQPYHAPDKPLDAPALLDFAQKYACQKGLTATLRG
CCEEECEEEEECEEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARAQDLLYDARLCLEGCDLCAQ
CEEECEECCCCCCCCCEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAPDVIERALNGLLIHREKLTDAHFSILAHCCPTQALTVCGEIKSVDEIMATVLRDKPFY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DRSGGGLTLSGGEPFMQPELAAELFKASHDAGIHTAVETCLHVPWKYIAPSLPYIDLFLA
CCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCCHHHHHHH
DLKHVADGPFKQWTDGSASRVLENLRKLAAAGKKMVIRVPLIQGFNADEEAIKAITDFAA
HHHHHCCCCCHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
DELHVGEIHFLPYHTLGINKYHLLSQPYHAPDKPLDAPALLDFAQKYACQKGLTATLRG
CCEEECEEEEECEEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]