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The map label for this gene is pflF [H]

Identifier: 16764205

GI number: 16764205

Start: 911347

End: 913779

Strand: Reverse

Name: pflF [H]

Synonym: STM0843

Alternate gene names: 16764205

Gene position: 913779-911347 (Counterclockwise)

Preceding gene: 16764206

Following gene: 16764203

Centisome position: 18.81

GC content: 55.65

Gene sequence:

>2433_bases
ATGACCCAACTGAAACTGGACACGCTCAGTGACCGCATTAAGGCGCACAAAACCGCGCTGGTACATATCGTGAAACCGCC
GGTCTGTACCGAGCGCGCGCAGCATTATACCGAAATGTACCAGCAGCATCTGGATAAACCGATTCCGGTACGCCGTGCGC
TGGCGCTGGCCCATCACCTGGCGGAACGCACTATCTGGATAAAACACGATGAACTGATCGTCGGCAACCAGGCAAGTGAA
GTCCGCGCCGCGCCGATTTTCCCGGAATATACCGTCTCGTGGATTGAAAAAGAGATCGACGACCTGGCGGACCGGCCCGG
CGCAGGTTTTTCCGTAAGTGAAGAAAACAAACGTATTCTGCACGACGTCTGTCCGTGGTGGCGCGGTCAAACCGTCCAGG
ATCGCTGCTACGGCATGTTTACCGATGAACAAAAAGGGCTTCTGGCGACTGGCATTATCAAAGCCGAAGGCAATATGACT
TCCGGCGATGCGCACCTGGCGGTGAACTTCCCGCTGCTGCTGGAAAAAGGGCTTTATGGCCTGCGCGATAAAGTCGCCGA
GCGCCGCTCACGCATCAACCTGACCGTGCTGGAAGATCTGCATGGCGAGCAGTTCCTGAAGGCGATTGATATTGTGCTGG
ACGCGGTAAGCCAGCATATCACGCGCTTTGCCGCGCTGGCGCGCCAGATGGCGGGCGAAGAGTCCCGTGAAAGCCGTCGT
AAAGAGCTACTTACCATCGCCGAAAACTGCGAGGTGATCGCCCACCAACCGCCGCAGACCTTCTGGCAAGCACTGCAATT
GTGCTACTTCATCCAACTGATTCTACAAATTGAGTCTAACGGTCACTCGGTGTCATTTGGTCGTATGGACCAGTATCTTT
ACCCCTATTATCGTCGCGATGTCGAACTAAACCAGACGCTGGATCGTGAGCACGCCATTGAACTGCTGCACAGCTGCTGG
CTGAAACTGCTGGAAGTAAACAAGATCCGCTCCGGTTCGCACTCTAAAGCCTCCGCGGGCAGCCCGCTGTATCAAAACGT
CACCATTGGCGGTCAGAAACTTATCAACGGTCAGCCAATGGATGCAGTGAATCCGCTGTCTTACGCCATTCTGGAGTCCT
GTGGACGTCTGCGTTCTACCCAGCCCAACCTCAGTGTGCGTTATCACGCCGGAATGAGTAACGATTTCCTTGACGCCTGC
GTCCAGGTCATCCGCTGCGGCTTTGGGATGCCGGCGTTTAACAATGATGAAATCGTCATCCCGGAATTTATCAAGTTGGG
GATCGAACCGCAGGACGCTTACGATTATGCGGCGATCGGCTGTATCGAAACCGCCGTCGGCGGGAAATGGGGTTATCGCT
GCACCGGCATGAGCTTTATTAACTTCGCCCGCGTCATGCTGGCGGCGCTGGAAGGCGGTCGCGACGCCACCAGCGGCAAG
GTATTTCTGCCGCAGGAAAAGGCGCTCTCCGCAGGCAACTTTAACAATTTTGACGAAGTGATGGCCGCCTGGGATACCCA
AATTCGCTACTACACGCGCAAATCGATTGAAATTGAGTATGTGGTCGACACCATGTTGGAAGAGAACGTCCACGATATCC
TCTGCTCAGCGCTGGTAGATGACTGTATTGAGCGAGCGAAAAGTATCAAGCAAGGCGGCGCGAAATATGACTGGGTATCC
GGTCTCCAGGTCGGTATCGCCAACCTGGGCAACAGCCTCGCCGCTGTGAAAAAACTGGTCTTCGAACAGGGCGTTATCGG
TCAACAGCAGCTTGCCGCCGCGCTGGCCGATGACTTTGACGGGCTGACCCATGAGCAGCTGCGCCAGCGTCTGATTAACG
GCGCGCCGAAGTACGGTAACGACGATGATACCGTCGATACGCTGCTGGCGCGCGCTTATCAGACCTATATTGATGAGCTG
AAGCAATATCATAACCCGCGCTACGGTCGTGGCCCTGTCGGCGGCAACTATTACGCCGGTACGTCCTCTATCTCAGCAAA
CGTGCCATTTGGCGCCGCGACTATGGCTACCCCGGATGGTCGCAAAGCGCATACACCGCTGGCGGAAGGCGCAAGCCCTG
CATCAGGTACGGATCATCTCGGCCCGACGGCGGTGATTGGTTCCGTAGGCAAATTACCTACCGGTTCCATTCTCGGCGGC
GTGCTGCTCAACCAGAAACTGAACCCAACGACGCTGGAAAACGAATCCGACAAACAGAAACTGATGGTTCTGCTACGGAC
GTTCTTTGAGGTGCATAAAGGCTGGCATATTCAGTACAACATCGTGTCACGCGAAACGCTGTTGGATGCGAAAAAACATC
CAGACCAGTACCGCGATCTGGTCGTGCGTGTGGCAGGCTACTCCGCCTTCTTCACCGCGCTGTCGCCGGATGCGCAGGAC
GATATCATCGCCCGTACCGAACATATGCTGTAA

Upstream 100 bases:

>100_bases
ATGCCCCGGATAAACCACTGGATGCGCCGGCGTTACTTGATTTTGCCCAGAAGTACGCCTGCCAAAAAGGTTTAACCGCG
ACCCTACGAGGATAATTATC

Downstream 100 bases:

>100_bases
TTTACCCTGGCGCCATCGGCAATATGTCCGAACTGCCTGATGGCGCTACGTTTAACAGGCCTACGCAGGCCTGTTAATCA
GAAGGGATCGGTGCCGTCAA

Product: pyruvate formate lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MTQLKLDTLSDRIKAHKTALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLAERTIWIKHDELIVGNQASE
VRAAPIFPEYTVSWIEKEIDDLADRPGAGFSVSEENKRILHDVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
SGDAHLAVNFPLLLEKGLYGLRDKVAERRSRINLTVLEDLHGEQFLKAIDIVLDAVSQHITRFAALARQMAGEESRESRR
KELLTIAENCEVIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCW
LKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQKLINGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDAC
VQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
VFLPQEKALSAGNFNNFDEVMAAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVS
GLQVGIANLGNSLAAVKKLVFEQGVIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDEL
KQYHNPRYGRGPVGGNYYAGTSSISANVPFGAATMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTGSILGG
VLLNQKLNPTTLENESDKQKLMVLLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDAQD
DIIARTEHML

Sequences:

>Translated_810_residues
MTQLKLDTLSDRIKAHKTALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLAERTIWIKHDELIVGNQASE
VRAAPIFPEYTVSWIEKEIDDLADRPGAGFSVSEENKRILHDVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
SGDAHLAVNFPLLLEKGLYGLRDKVAERRSRINLTVLEDLHGEQFLKAIDIVLDAVSQHITRFAALARQMAGEESRESRR
KELLTIAENCEVIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCW
LKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQKLINGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDAC
VQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
VFLPQEKALSAGNFNNFDEVMAAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVS
GLQVGIANLGNSLAAVKKLVFEQGVIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDEL
KQYHNPRYGRGPVGGNYYAGTSSISANVPFGAATMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTGSILGG
VLLNQKLNPTTLENESDKQKLMVLLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDAQD
DIIARTEHML
>Mature_809_residues
TQLKLDTLSDRIKAHKTALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLAERTIWIKHDELIVGNQASEV
RAAPIFPEYTVSWIEKEIDDLADRPGAGFSVSEENKRILHDVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTS
GDAHLAVNFPLLLEKGLYGLRDKVAERRSRINLTVLEDLHGEQFLKAIDIVLDAVSQHITRFAALARQMAGEESRESRRK
ELLTIAENCEVIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWL
KLLEVNKIRSGSHSKASAGSPLYQNVTIGGQKLINGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACV
QVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGKV
FLPQEKALSAGNFNNFDEVMAAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSG
LQVGIANLGNSLAAVKKLVFEQGVIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELK
QYHNPRYGRGPVGGNYYAGTSSISANVPFGAATMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTGSILGGV
LLNQKLNPTTLENESDKQKLMVLLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDD
IIARTEHML

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=810, Percent_Identity=95.8024691358025, Blast_Score=1631, Evalue=0.0,
Organism=Escherichia coli, GI1790388, Length=805, Percent_Identity=37.5155279503106, Blast_Score=480, Evalue=1e-136,
Organism=Escherichia coli, GI1787131, Length=563, Percent_Identity=26.9982238010657, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI48994926, Length=564, Percent_Identity=28.1914893617021, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788933, Length=56, Percent_Identity=50, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90175; Mature: 90043

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLKLDTLSDRIKAHKTALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHL
CCCEEHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AERTIWIKHDELIVGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFSVSEENKRIL
HHCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
HDVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLYG
HHHCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECHHHHHHCHHH
LRDKVAERRSRINLTVLEDLHGEQFLKAIDIVLDAVSQHITRFAALARQMAGEESRESRR
HHHHHHHHHHCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KELLTIAENCEVIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRD
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
VELNQTLDREHAIELLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQKLINGQPM
CCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHCCCCCCC
DAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVI
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEC
PEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCC
VFLPQEKALSAGNFNNFDEVMAAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVD
EECCHHHHCCCCCCCCHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHH
DCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGVIGQQQLAAALADDFD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAG
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
TSSISANVPFGAATMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTGSILGG
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHH
VLLNQKLNPTTLENESDKQKLMVLLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCHHHHHH
VVRVAGYSAFFTALSPDAQDDIIARTEHML
HHHHHCHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TQLKLDTLSDRIKAHKTALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHL
CCEEHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AERTIWIKHDELIVGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFSVSEENKRIL
HHCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
HDVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLYG
HHHCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECHHHHHHCHHH
LRDKVAERRSRINLTVLEDLHGEQFLKAIDIVLDAVSQHITRFAALARQMAGEESRESRR
HHHHHHHHHHCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KELLTIAENCEVIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRD
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
VELNQTLDREHAIELLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQKLINGQPM
CCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHCCCCCCC
DAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVI
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEC
PEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCC
VFLPQEKALSAGNFNNFDEVMAAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVD
EECCHHHHCCCCCCCCHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHH
DCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGVIGQQQLAAALADDFD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAG
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
TSSISANVPFGAATMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTGSILGG
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHH
VLLNQKLNPTTLENESDKQKLMVLLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCHHHHHH
VVRVAGYSAFFTALSPDAQDDIIARTEHML
HHHHHCHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]