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The map label for this gene is proB

Identifier: 167625082

GI number: 167625082

Start: 3871584

End: 3872702

Strand: Reverse

Name: proB

Synonym: Shal_3170

Alternate gene names: 167625082

Gene position: 3872702-3871584 (Counterclockwise)

Preceding gene: 167625083

Following gene: 167625081

Centisome position: 74.09

GC content: 48.35

Gene sequence:

>1119_bases
ATGAACTTAAGTGAGATTAGTTACCGCCGCGTAGTGGTTAAATTGGGAACAAGTGTACTGACATCTGGCAGCTTAAAGTT
AGATAAAGCGCACATGGTTGAATTAGCACGACAGATGGCTTGCTTGATGAAAGCAGGTGTTGAGGTCGTATTGTGTACCT
CGGGAGCAATCGCTGCGGGTAAAGAGCATTTAGGCTACCCCCAATTACCCGATACCATAGCCAGTAAACAGCTGCTCGCT
GCGGTAGGGCAAAGCCAGCTAATCCTTGCTTGGTCTCAGTTATTTAGTATTTATGGTTTGCACGTTGGCCAGCTATTGCT
GACCCGCGCCGATCTGCATGACAGGGAGCGCTATTTAAACGCTAGAGACTCTCTTAATGCGCTGCTTAACAATGGCATTA
TCCCAATTATTAATGAAAATGACGCCGTAGCAACGGCCGAAATTAAAGTTGGCGACAACGATAATCTGTCTGCTAGAGCC
GCACTGCTATGCGATGCAGATTTGTTGATCTTATTGACCGACCAAAAAGGTCTATTTGACGCCGACCCGCGTAAAAACCC
TGATGCAAAGCTGATCACCGAAGTCCAAAACATCGATGATAGCCTACGTATGTTGGCTGGGGGCGCGGTTTCAGGCCTTG
GTACGGGTGGAATGGCGACTAAGCTTGAAGCTGCGGATATTGCACGTCGTGCAGGTGTTGAAGTGGTGATCGCATCGGGA
CATCATAAAGATGTCATTCAAAATGTAGTGTGCGCAAAGCCTGTCGGTACCCATTTTACTGCGCTTGAGCATCCGCTTGA
GAGCCGTAAACAGTGGATTCTGGCCGGGCCTAAGGCTCGTGGTCAAGTCGTGCTGGATACTGGTGCAATTACAGCGGTAA
CCCAGAAAGGCAGAAGCTTGCTCTCTAAAGGGATCATCGAGGTTAACGGGCTATTTCAGCGCGGCGATACCATAGAACTT
GTTGATACTAAGGGTAAGGTTTACGCCAAGGGCATGAGTCGATACGGCTCGCTTGATGTCAGTAAGATTGCTGGTAAGCA
CTCTGATAACATCGAAGAGATACTAGGCTACGATTATGGTGATGCAGTAGTTCACCGCAACGACATGGTGGTGTTATGA

Upstream 100 bases:

>100_bases
CTAATTCAGCTTTTTTTTCAGTTTCTTTGTTGGTGCTTACTACCATTTCAAACCTAGATTGGGTATAAGGTTTTATTCAA
TGTAATTTTGTTAGCAAATA

Downstream 100 bases:

>100_bases
ATAACGACGAATATTTAACGGCTTTAGGCCAAAATGCAAAGCAGGCGAGCTATGCCTTGGCCACGCTTTCGGGTCAACAG
AAGTCAGCGCTATTACGCTG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNLSEISYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAGKEHLGYPQLPDTIASKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
HHKDVIQNVVCAKPVGTHFTALEHPLESRKQWILAGPKARGQVVLDTGAITAVTQKGRSLLSKGIIEVNGLFQRGDTIEL
VDTKGKVYAKGMSRYGSLDVSKIAGKHSDNIEEILGYDYGDAVVHRNDMVVL

Sequences:

>Translated_372_residues
MNLSEISYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAGKEHLGYPQLPDTIASKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
HHKDVIQNVVCAKPVGTHFTALEHPLESRKQWILAGPKARGQVVLDTGAITAVTQKGRSLLSKGIIEVNGLFQRGDTIEL
VDTKGKVYAKGMSRYGSLDVSKIAGKHSDNIEEILGYDYGDAVVHRNDMVVL
>Mature_372_residues
MNLSEISYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAGKEHLGYPQLPDTIASKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
HHKDVIQNVVCAKPVGTHFTALEHPLESRKQWILAGPKARGQVVLDTGAITAVTQKGRSLLSKGIIEVNGLFQRGDTIEL
VDTKGKVYAKGMSRYGSLDVSKIAGKHSDNIEEILGYDYGDAVVHRNDMVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=36.3945578231293, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=36.1486486486487, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=62.5, Blast_Score=448, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=34.0823970037453, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=34.0823970037453, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=37.1084337349398, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=411, Percent_Identity=34.0632603406326, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=34.1379310344828, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_SHEHH (B0TQC5)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675376.1
- ProteinModelPortal:   B0TQC5
- SMR:   B0TQC5
- GeneID:   5904757
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3170
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   SHAL458817:SHAL_3170-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39827; Mature: 39827

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEISYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAG
CCCCHHHHHHHHHHHCHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
KEHLGYPQLPDTIASKQLLAAVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLN
HHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
ARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARAALLCDADLLILLTDQKGLFD
HHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
CCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECC
HHKDVIQNVVCAKPVGTHFTALEHPLESRKQWILAGPKARGQVVLDTGAITAVTQKGRSL
CCHHHHHHHHHCCCCCCCHHHHHCCHHHCCEEEEECCCCCCEEEEECCCCHHHHHHHHHH
LSKGIIEVNGLFQRGDTIELVDTKGKVYAKGMSRYGSLDVSKIAGKHSDNIEEILGYDYG
HHCCCEEECCEECCCCEEEEEECCCCEEECHHHHCCCCCHHHHCCCCCCCHHHHHCCCCC
DAVVHRNDMVVL
CEEEECCCEEEC
>Mature Secondary Structure
MNLSEISYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAG
CCCCHHHHHHHHHHHCHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
KEHLGYPQLPDTIASKQLLAAVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLN
HHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
ARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARAALLCDADLLILLTDQKGLFD
HHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
CCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECC
HHKDVIQNVVCAKPVGTHFTALEHPLESRKQWILAGPKARGQVVLDTGAITAVTQKGRSL
CCHHHHHHHHHCCCCCCCHHHHHCCHHHCCEEEEECCCCCCEEEEECCCCHHHHHHHHHH
LSKGIIEVNGLFQRGDTIELVDTKGKVYAKGMSRYGSLDVSKIAGKHSDNIEEILGYDYG
HHCCCEEECCEECCCCEEEEEECCCCEEECHHHHCCCCCHHHHCCCCCCCHHHHHCCCCC
DAVVHRNDMVVL
CEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA