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The map label for this gene is folC [H]

Identifier: 167624560

GI number: 167624560

Start: 3200947

End: 3202212

Strand: Reverse

Name: folC [H]

Synonym: Shal_2640

Alternate gene names: 167624560

Gene position: 3202212-3200947 (Counterclockwise)

Preceding gene: 167624561

Following gene: 167624559

Centisome position: 61.26

GC content: 47.31

Gene sequence:

>1266_bases
ATGCCAGTAGCACCAAAGTCAGGTGCTACGTTAAACCGCTGGTTAGATTTTTTATTAGCGATTCATCCAACCGAAATTGA
TATGGGCCTTGGGCGTGTATCACAGGTTGCTAAACGCATGGGCTTAACCGATTTAGGTGAGACCAAGGTTGTCACTGTAG
CAGGCACCAATGGCAAGGGAACCACCTGCGCCATGATTGAAGCTGCATTGCAGTTGTCAGGACAAACCGTCGGTGTTTAC
AGCTCTCCTCATCTGCTGAAGTACAATGAACGCGTGCGCATAAATGGCCTTGATGCGACAGATGCAGCGTTAGTTAATGC
ATTTGAATCGATTGAAACCGCGCGCGCAGAGATCTCATTAACCTTTTTTGAATACGCGACACTAGCAGGTCTTTATCTTT
TTAAAGCCGCTAAGTTGGACGTGATATTGCTCGAAGTTGGTCTGGGCGGTCGACTAGACGCCACCAATATTATCGATACC
GATATTGCGGTCGTGACCTCTATCGATTTAGATCATCAAGAGTACCTCGGTGATACGAGGGAGCACGTTGCTGCAGAAAA
AGCGGGTATATTCAGAGCCAATCGTCCGGCAATCGTGGGTGAGCCTGATTGCCCAAGCTCGCTGCTTGATGTAGCCAATC
GAGTTGGTGCATCACTTTACCGAGTCAATAGTGAGTTCAGTTATCGTTGCAGCGATAAAGATTGGCACTATCAAGGCCAA
CATTTCGACCTAGATAACTTGCCGTTCCCTTATTTGCCTCAACCTAATGCCGCCACGGTTATTGCGGTACTTGAGCATCT
TTGCCCCACTATTTCAGAGGATATTATTGCTAAAGCAGTGGGAGAGACCCAACTTGCGGGGCGATTAGAGTGTCTTAATG
ATTCTCCAAGAGTGATTGTTGATGTCGCACATAACCCTCATGCCGCGCGTTATTTAAAGTCACGTTTAGCCGCGATGCCA
AAGCGTCGCTTAATTGCACTTTGTGGCATGTTAAAAGATAAAGATGTGCGCTCTGTGCTACAGGAGCTATCGGCAGATGT
TGATCAGTGGTATTTGTGCGATCTGTCTGTGCCACGGGGAGCCACCGCTGCAGACTTGAAAGCATTCATGCCTGCACAAA
CACAAGCTTCAACTTATACCGATATGAGTGCCGCCTGGCATGCGCTTCGAGCGCAATTGCAGCCTGATGATGTGGTAATT
GTATTTGGCTCATTTTACACTGTTGCAGGTTTTAAAGATTTGTTTAAGGGAGAGAGTCTTGTCTAG

Upstream 100 bases:

>100_bases
TAAGGCATGTTTAATGAGGGTTAAGGATAATCTTAACGGTAAAGGTTTGGGCCTTTAGGTTTTAAATGGATAAAAGTTTG
TGATTGAACAAGACACATTA

Downstream 100 bases:

>100_bases
TCAATTTCAAAACCGTCTTGTCGGTGTCATCGTTATTGTGGCATTAGGGGTTATATTTTTACCCGATATTTTGGATGGTA
AAAAACAGCGCGAAGAAGAA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPVAPKSGATLNRWLDFLLAIHPTEIDMGLGRVSQVAKRMGLTDLGETKVVTVAGTNGKGTTCAMIEAALQLSGQTVGVY
SSPHLLKYNERVRINGLDATDAALVNAFESIETARAEISLTFFEYATLAGLYLFKAAKLDVILLEVGLGGRLDATNIIDT
DIAVVTSIDLDHQEYLGDTREHVAAEKAGIFRANRPAIVGEPDCPSSLLDVANRVGASLYRVNSEFSYRCSDKDWHYQGQ
HFDLDNLPFPYLPQPNAATVIAVLEHLCPTISEDIIAKAVGETQLAGRLECLNDSPRVIVDVAHNPHAARYLKSRLAAMP
KRRLIALCGMLKDKDVRSVLQELSADVDQWYLCDLSVPRGATAADLKAFMPAQTQASTYTDMSAAWHALRAQLQPDDVVI
VFGSFYTVAGFKDLFKGESLV

Sequences:

>Translated_421_residues
MPVAPKSGATLNRWLDFLLAIHPTEIDMGLGRVSQVAKRMGLTDLGETKVVTVAGTNGKGTTCAMIEAALQLSGQTVGVY
SSPHLLKYNERVRINGLDATDAALVNAFESIETARAEISLTFFEYATLAGLYLFKAAKLDVILLEVGLGGRLDATNIIDT
DIAVVTSIDLDHQEYLGDTREHVAAEKAGIFRANRPAIVGEPDCPSSLLDVANRVGASLYRVNSEFSYRCSDKDWHYQGQ
HFDLDNLPFPYLPQPNAATVIAVLEHLCPTISEDIIAKAVGETQLAGRLECLNDSPRVIVDVAHNPHAARYLKSRLAAMP
KRRLIALCGMLKDKDVRSVLQELSADVDQWYLCDLSVPRGATAADLKAFMPAQTQASTYTDMSAAWHALRAQLQPDDVVI
VFGSFYTVAGFKDLFKGESLV
>Mature_420_residues
PVAPKSGATLNRWLDFLLAIHPTEIDMGLGRVSQVAKRMGLTDLGETKVVTVAGTNGKGTTCAMIEAALQLSGQTVGVYS
SPHLLKYNERVRINGLDATDAALVNAFESIETARAEISLTFFEYATLAGLYLFKAAKLDVILLEVGLGGRLDATNIIDTD
IAVVTSIDLDHQEYLGDTREHVAAEKAGIFRANRPAIVGEPDCPSSLLDVANRVGASLYRVNSEFSYRCSDKDWHYQGQH
FDLDNLPFPYLPQPNAATVIAVLEHLCPTISEDIIAKAVGETQLAGRLECLNDSPRVIVDVAHNPHAARYLKSRLAAMPK
RRLIALCGMLKDKDVRSVLQELSADVDQWYLCDLSVPRGATAADLKAFMPAQTQASTYTDMSAAWHALRAQLQPDDVVIV
FGSFYTVAGFKDLFKGESLV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=194, Percent_Identity=36.5979381443299, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI66932990, Length=194, Percent_Identity=36.5979381443299, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1788654, Length=406, Percent_Identity=50.2463054187192, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71984923, Length=160, Percent_Identity=33.75, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17553150, Length=160, Percent_Identity=33.75, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17553148, Length=160, Percent_Identity=33.75, Blast_Score=111, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=421, Percent_Identity=25.6532066508314, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324815, Length=211, Percent_Identity=32.7014218009479, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24641571, Length=190, Percent_Identity=34.2105263157895, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24581568, Length=205, Percent_Identity=33.1707317073171, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45840; Mature: 45709

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAPKSGATLNRWLDFLLAIHPTEIDMGLGRVSQVAKRMGLTDLGETKVVTVAGTNGKG
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCC
TTCAMIEAALQLSGQTVGVYSSPHLLKYNERVRINGLDATDAALVNAFESIETARAEISL
CHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHEEEE
TFFEYATLAGLYLFKAAKLDVILLEVGLGGRLDATNIIDTDIAVVTSIDLDHQEYLGDTR
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCCHHHHHCCHH
EHVAAEKAGIFRANRPAIVGEPDCPSSLLDVANRVGASLYRVNSEFSYRCSDKDWHYQGQ
HHHHHHHHCCEECCCCCEECCCCCCHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCCCC
HFDLDNLPFPYLPQPNAATVIAVLEHLCPTISEDIIAKAVGETQLAGRLECLNDSPRVIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHEECCCCCCEEE
DVAHNPHAARYLKSRLAAMPKRRLIALCGMLKDKDVRSVLQELSADVDQWYLCDLSVPRG
EECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
ATAADLKAFMPAQTQASTYTDMSAAWHALRAQLQPDDVVIVFGSFYTVAGFKDLFKGESL
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCC
V
C
>Mature Secondary Structure 
PVAPKSGATLNRWLDFLLAIHPTEIDMGLGRVSQVAKRMGLTDLGETKVVTVAGTNGKG
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCC
TTCAMIEAALQLSGQTVGVYSSPHLLKYNERVRINGLDATDAALVNAFESIETARAEISL
CHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHEEEE
TFFEYATLAGLYLFKAAKLDVILLEVGLGGRLDATNIIDTDIAVVTSIDLDHQEYLGDTR
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCCHHHHHCCHH
EHVAAEKAGIFRANRPAIVGEPDCPSSLLDVANRVGASLYRVNSEFSYRCSDKDWHYQGQ
HHHHHHHHCCEECCCCCEECCCCCCHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCCCC
HFDLDNLPFPYLPQPNAATVIAVLEHLCPTISEDIIAKAVGETQLAGRLECLNDSPRVIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHEECCCCCCEEE
DVAHNPHAARYLKSRLAAMPKRRLIALCGMLKDKDVRSVLQELSADVDQWYLCDLSVPRG
EECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
ATAADLKAFMPAQTQASTYTDMSAAWHALRAQLQPDDVVIVFGSFYTVAGFKDLFKGESL
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]