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The map label for this gene is truA

Identifier: 167624561

GI number: 167624561

Start: 3202310

End: 3203095

Strand: Reverse

Name: truA

Synonym: Shal_2641

Alternate gene names: 167624561

Gene position: 3203095-3202310 (Counterclockwise)

Preceding gene: 167624562

Following gene: 167624560

Centisome position: 61.28

GC content: 45.55

Gene sequence:

>786_bases
ATGCGGGTTGCGCTAGGTATAGAGTACGATGGTAGTCAATATTTCGGATGGCAACGTCAGGCCGAAGTGGACACTGTACA
AGAGCGGCTAGAAAAAGCGCTTTCGATCGTTGCTAATGAACCTATTGCGGTGCAATGTGCGGGTCGTACTGACGCGGGGG
TTCACGCGACAGGACAAGTGGTTCATTTTGAAACGAATGCCGTGCGCAAGGATACCGCTTGGACACTTGGCGTGAATGTG
AACCTGCCAGATAATATTGCAGTGCGCTGGGTTAAAGTCGTTGATGATGAGTTTCATGCTCGTTTTACGGCAACGGCAAG
ACGTTATCGTTACATGATTTATAATCATCAGCTTCGTCCAGGCATTTTACGCAGTGGCGTAAGCCATTACCCTGGTGTTA
TCGACGAAGCCAAGATGCACCAAGCGGCGCAGTATCTTCTGGGTGAACAAGATTTTACTAGCTTTAGAGCCGTACAGTGC
CAGTCCAATACACCGTTTCGCTGTGTACATGAAGTCAATGTGAGCCGTCAGGGTATGTATATCTGCGTTGATATCAAGGC
CAATGCATTCTTGCATCACATGGTGCGTAATATTGTCGGTTCGTTATTAGAGGTTGGTTTAGGTAATCAACCTATTGAGT
GGATTGAGCAATTGCTTGCACTAAAAGATAGAAATAAAGCTGCAGCAACAGCCAAACCTAATGGTCTGTATTTAGTGGAT
GTCACTTATCCTGAAAGTTACCAATTACCTAAGCTATCACTTGGTCCATTATTTATGTTGGACTAA

Upstream 100 bases:

>100_bases
GCTAGAATCGCCGCCTATTAAAAGATTTCGTTTATCCGATCTGTTCGCCAAATACGGTGGGCGGTCGAATAGCAGTTTAA
CCGAGTTATAGAGGTGTGAG

Downstream 100 bases:

>100_bases
GGCATGTTTAATGAGGGTTAAGGATAATCTTAACGGTAAAGGTTTGGGCCTTTAGGTTTTAAATGGATAAAAGTTTGTGA
TTGAACAAGACACATTAATG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVQCAGRTDAGVHATGQVVHFETNAVRKDTAWTLGVNV
NLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRPGILRSGVSHYPGVIDEAKMHQAAQYLLGEQDFTSFRAVQC
QSNTPFRCVHEVNVSRQGMYICVDIKANAFLHHMVRNIVGSLLEVGLGNQPIEWIEQLLALKDRNKAAATAKPNGLYLVD
VTYPESYQLPKLSLGPLFMLD

Sequences:

>Translated_261_residues
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVQCAGRTDAGVHATGQVVHFETNAVRKDTAWTLGVNV
NLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRPGILRSGVSHYPGVIDEAKMHQAAQYLLGEQDFTSFRAVQC
QSNTPFRCVHEVNVSRQGMYICVDIKANAFLHHMVRNIVGSLLEVGLGNQPIEWIEQLLALKDRNKAAATAKPNGLYLVD
VTYPESYQLPKLSLGPLFMLD
>Mature_261_residues
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVQCAGRTDAGVHATGQVVHFETNAVRKDTAWTLGVNV
NLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRPGILRSGVSHYPGVIDEAKMHQAAQYLLGEQDFTSFRAVQC
QSNTPFRCVHEVNVSRQGMYICVDIKANAFLHHMVRNIVGSLLEVGLGNQPIEWIEQLLALKDRNKAAATAKPNGLYLVD
VTYPESYQLPKLSLGPLFMLD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=269, Percent_Identity=27.8810408921933, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=71.5384615384615, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_SHEHH (B0TKZ4)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674855.1
- ProteinModelPortal:   B0TKZ4
- SMR:   B0TKZ4
- GeneID:   5906521
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2641
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- ProtClustDB:   PRK00021
- BioCyc:   SHAL458817:SHAL_2641-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29402; Mature: 29402

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVQCAGRTDAGVHATGQV
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCEE
VHFETNAVRKDTAWTLGVNVNLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRP
EEEECCCCCCCCEEEEEEEECCCCCHHEEEEEEECCCHHHHHHHHHHHEEEEEEECCCCC
GILRSGVSHYPGVIDEAKMHQAAQYLLGEQDFTSFRAVQCQSNTPFRCVHEVNVSRQGMY
HHHHCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCEE
ICVDIKANAFLHHMVRNIVGSLLEVGLGNQPIEWIEQLLALKDRNKAAATAKPNGLYLVD
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
VTYPESYQLPKLSLGPLFMLD
EECCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVQCAGRTDAGVHATGQV
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCEE
VHFETNAVRKDTAWTLGVNVNLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRP
EEEECCCCCCCCEEEEEEEECCCCCHHEEEEEEECCCHHHHHHHHHHHEEEEEEECCCCC
GILRSGVSHYPGVIDEAKMHQAAQYLLGEQDFTSFRAVQCQSNTPFRCVHEVNVSRQGMY
HHHHCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCEE
ICVDIKANAFLHHMVRNIVGSLLEVGLGNQPIEWIEQLLALKDRNKAAATAKPNGLYLVD
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
VTYPESYQLPKLSLGPLFMLD
EECCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA