Click here to switch to the map view.

The map label for this gene is phnE [H]

Identifier: 167624450

GI number: 167624450

Start: 3057701

End: 3059254

Strand: Reverse

Name: phnE [H]

Synonym: Shal_2528

Alternate gene names: 167624450

Gene position: 3059254-3057701 (Counterclockwise)

Preceding gene: 167624451

Following gene: 167624448

Centisome position: 58.53

GC content: 46.72

Gene sequence:

>1554_bases
TTGCCAAAACAAAGCCTTACGCCGCCAACCTTGTTTGCATTTGTCGGCTACTGGAAAAAAGTGACCCTAACACTGTGGGC
GATAACACTGCTCAGTTTCTATTTTGCCGACACCGAAGTGATAGCCCTCGACCCATGGAGCGAGTTATTACTCATAGGTA
AAGGTTTTATTGCCCCGGACTTTTTTGCCACCGAATATTTATTCGATGCCTTATGGCAAACCGTTAGCTTTGCGCTGCTA
GGTGTATGCTTGGCGCTTGTGTGGGGCGCACCCGTTTCACTTATCTACCACAAACCTTATATTGCAGCCTTGTGTGCATT
TATTCGCTCTATTCATGAGATTTTTTGGGCACTGCTATTTTTACAAATTTTTGGTCTATCACCACTGACCGGTATTCTAG
CCATCGCACTGCCGTATGGCGCAACCTTTGCGCGGGTGTTTAGCGATATCTTGCAGCAAGCCCCTACAACAAGCTTGCAA
ACGTTTCCAAACCAGACAGACCGCATTAGCCGCTATTTATATGGCCGAATCGCGCATGTTTTTGTGCCTATTCGAGCCTA
TGTACGCTATCGGTTTGAGTGTGCCTTGAGAAGCAGCGCGGTGCTAGGTTTTGTCGGCATGCCAACCTTAGGTTTTTATT
TAGAATCGGCGTTCCGTCAGGGCCATTATCATCAAGGCGCAGCCTTACTCATGATGTTTATTGTCTTGATCGGCACAATT
CCATATTGGGGCCGAATAAAATTACTGCCAGTTTATTTTGTGATAGCGATATGGGCGTTGCCAACAATACCGGATGTTGA
CGGTGCGTTAATCTGGCGTTTTCTAAGCCAAGATATCTTGCCGCCCATGTTTCAAACCACGCTTCAGAGCCAAGCGTTAC
AAGCCAGTGCAATGAGTAGCGGTGATGCGTTTAGCCAGTTCCTTGATTGGATCAGCAGGCTACTCACAGAGCAAGCGTTG
CCCGGTGTGATAGCAACCTTAATACTTGCCCTTGCGGCATTGGGGATGAGCCACCTTTTTGCACTGATAGGTATTGCGGT
GAGTGACAAGCGCTTGAGCGGGCGCTTTGTTGCCAATATCAACAGCCTTGCGCTATTGATCCTGCGCTCTGTACCAGAAT
ATATTTTTGCCTTTGTGTTTATGTTGTTGCTTGGGCCATCAATGTTGCCAGCCATGTTAGCGTTGGCGATTCATAACGGT
GCGCTTATCGCTTACTTAACCATTCGTCATGCCGATGATGTCTCTGTGTCGGCGCACTTTAATGGCAGGCTCGATGGCTA
TGGTTACGAGGTCTTACCCGCAATATACCCAAACATGATGGCATTGCTATTTTATCGCTTCGAAGTGATCTTAAGAGAAA
CGGCAATTTTAGGTATGTTAGGCATCGCCACATTGGGATTTTATATCGACAGTAATTTTGAAGAGATCCGCTTTAATGGT
GCATTAGTGCTGATGTGCGTGACGGCTTTGGTCAATATTGTGGTTGATATCTTGAGTCGACGTGTCTTGTACAACAAAAA
CAAAACGCAACTAGGGTGTGCGCATCAGCAGTAG

Upstream 100 bases:

>100_bases
GCTTTCTATCAAGATGATACGGCACTCGATTTTATGGTGGCGGCTTCGCCAGCAACGAATCGTCTCGAATCGCTTCCACG
CGCTGTTAAGCGTTATTAGT

Downstream 100 bases:

>100_bases
AGCCTTGCTGAGGCTAGTTGCGTTTTTGATTAAGCTTATTTATTAACTGAAAATCTTACGTTGATTGCGTTTTTGAATGA
TGCCATTACCACACCAAGCG

Product: binding-protein-dependent transport systems inner membrane component

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MPKQSLTPPTLFAFVGYWKKVTLTLWAITLLSFYFADTEVIALDPWSELLLIGKGFIAPDFFATEYLFDALWQTVSFALL
GVCLALVWGAPVSLIYHKPYIAALCAFIRSIHEIFWALLFLQIFGLSPLTGILAIALPYGATFARVFSDILQQAPTTSLQ
TFPNQTDRISRYLYGRIAHVFVPIRAYVRYRFECALRSSAVLGFVGMPTLGFYLESAFRQGHYHQGAALLMMFIVLIGTI
PYWGRIKLLPVYFVIAIWALPTIPDVDGALIWRFLSQDILPPMFQTTLQSQALQASAMSSGDAFSQFLDWISRLLTEQAL
PGVIATLILALAALGMSHLFALIGIAVSDKRLSGRFVANINSLALLILRSVPEYIFAFVFMLLLGPSMLPAMLALAIHNG
ALIAYLTIRHADDVSVSAHFNGRLDGYGYEVLPAIYPNMMALLFYRFEVILRETAILGMLGIATLGFYIDSNFEEIRFNG
ALVLMCVTALVNIVVDILSRRVLYNKNKTQLGCAHQQ

Sequences:

>Translated_517_residues
MPKQSLTPPTLFAFVGYWKKVTLTLWAITLLSFYFADTEVIALDPWSELLLIGKGFIAPDFFATEYLFDALWQTVSFALL
GVCLALVWGAPVSLIYHKPYIAALCAFIRSIHEIFWALLFLQIFGLSPLTGILAIALPYGATFARVFSDILQQAPTTSLQ
TFPNQTDRISRYLYGRIAHVFVPIRAYVRYRFECALRSSAVLGFVGMPTLGFYLESAFRQGHYHQGAALLMMFIVLIGTI
PYWGRIKLLPVYFVIAIWALPTIPDVDGALIWRFLSQDILPPMFQTTLQSQALQASAMSSGDAFSQFLDWISRLLTEQAL
PGVIATLILALAALGMSHLFALIGIAVSDKRLSGRFVANINSLALLILRSVPEYIFAFVFMLLLGPSMLPAMLALAIHNG
ALIAYLTIRHADDVSVSAHFNGRLDGYGYEVLPAIYPNMMALLFYRFEVILRETAILGMLGIATLGFYIDSNFEEIRFNG
ALVLMCVTALVNIVVDILSRRVLYNKNKTQLGCAHQQ
>Mature_516_residues
PKQSLTPPTLFAFVGYWKKVTLTLWAITLLSFYFADTEVIALDPWSELLLIGKGFIAPDFFATEYLFDALWQTVSFALLG
VCLALVWGAPVSLIYHKPYIAALCAFIRSIHEIFWALLFLQIFGLSPLTGILAIALPYGATFARVFSDILQQAPTTSLQT
FPNQTDRISRYLYGRIAHVFVPIRAYVRYRFECALRSSAVLGFVGMPTLGFYLESAFRQGHYHQGAALLMMFIVLIGTIP
YWGRIKLLPVYFVIAIWALPTIPDVDGALIWRFLSQDILPPMFQTTLQSQALQASAMSSGDAFSQFLDWISRLLTEQALP
GVIATLILALAALGMSHLFALIGIAVSDKRLSGRFVANINSLALLILRSVPEYIFAFVFMLLLGPSMLPAMLALAIHNGA
LIAYLTIRHADDVSVSAHFNGRLDGYGYEVLPAIYPNMMALLFYRFEVILRETAILGMLGIATLGFYIDSNFEEIRFNGA
LVLMCVTALVNIVVDILSRRVLYNKNKTQLGCAHQQ

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 57608; Mature: 57477

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQSLTPPTLFAFVGYWKKVTLTLWAITLLSFYFADTEVIALDPWSELLLIGKGFIAPD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCCH
FFATEYLFDALWQTVSFALLGVCLALVWGAPVSLIYHKPYIAALCAFIRSIHEIFWALLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LQIFGLSPLTGILAIALPYGATFARVFSDILQQAPTTSLQTFPNQTDRISRYLYGRIAHV
HHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHH
FVPIRAYVRYRFECALRSSAVLGFVGMPTLGFYLESAFRQGHYHQGAALLMMFIVLIGTI
HHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
PYWGRIKLLPVYFVIAIWALPTIPDVDGALIWRFLSQDILPPMFQTTLQSQALQASAMSS
CCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GDAFSQFLDWISRLLTEQALPGVIATLILALAALGMSHLFALIGIAVSDKRLSGRFVANI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
NSLALLILRSVPEYIFAFVFMLLLGPSMLPAMLALAIHNGALIAYLTIRHADDVSVSAHF
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEE
NGRLDGYGYEVLPAIYPNMMALLFYRFEVILRETAILGMLGIATLGFYIDSNFEEIRFNG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECC
ALVLMCVTALVNIVVDILSRRVLYNKNKTQLGCAHQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PKQSLTPPTLFAFVGYWKKVTLTLWAITLLSFYFADTEVIALDPWSELLLIGKGFIAPD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCCH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HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]