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The map label for this gene is bioB

Identifier: 167624399

GI number: 167624399

Start: 2978318

End: 2979370

Strand: Reverse

Name: bioB

Synonym: Shal_2477

Alternate gene names: 167624399

Gene position: 2979370-2978318 (Counterclockwise)

Preceding gene: 167624402

Following gene: 167624398

Centisome position: 57.0

GC content: 45.96

Gene sequence:

>1053_bases
ATGTCTGAAGTACAACTAAGAAATAACTGGAAGCGTGAAGAGATTGAAAGCTTATTTGCGCTGCCAATGAACGATCTTCT
ATTTCAGGCGCATAGCATTCATCGCCAAGAGTTCGATCCAAATGAAGTTCAGATAAGCCGTTTATTGTCCATTAAAACCG
GTGCTTGCCCTGAGGATTGTAAATATTGCCCACAAAGCGCACGTTATGACACCGGTCTTGAAAAAGAGCGCTTACTCGCC
ATGGAAACTGTATTGACCGAAGCGCGCAGTGCTAAAGCCGCTGGAGCATCTCGTTTCTGTATGGGCGCAGCTTGGCGTAA
TCCGAAAGAGCGTGATATGCCTTACCTTAAGACTATGGTAGAAGAAGTTAAAGCATTGGGCATGGAAACCTGTATGACCC
TTGGCATGTTGTCTGCGGAGCAAGCCAATACACTTGCTGATGCCGGTTTGGATTATTACAACCATAACTTAGACACATCG
CCTGAATATTATGGCGATGTGATCACAACTCGCACTTATCAGAGTCGTTTAGATACCTTATCCAATGTACGCGCATCAGG
CATGAAAGTGTGTTCTGGCGGTATTGTTGGCATGGGCGAGAAAGCGACCGACCGAGCGGGTCTCATTCAGCAATTGGCAA
ACTTAGATCAGCACCCAGATTCTGTGCCGATCAATATGTTGGTTAAAGTTGAAGGTACGCCATTTGAAAAGCTAGATGAT
TTAGACCCATTAGAGTTTGTTCGTACCATTGCTGTTGCACGTATTACTATGCCCAAGTCTAGAGTGCGCTTATCGGCTGG
TCGTGAGAATATGAGCGATGAACTGCAAGCGATGTGCTTTTTTGCGGGAGCCAATTCAATCTTCTACGGTTGCAAGCTGC
TAACTACGCCCAACCCAGAAGAAAATGATGATATGGGTCTATTTAGACGACTGGGGCTCCATCCAGAGCAGGGCGTAGCC
TCAACTAAAGAACAAGATGAAGCCATGTTGGCAAAAGCTGCTGCGCAGCAAGACAAGAAAGTGTCAGCATTTTATGATGC
TGGTGCGCTTTAA

Upstream 100 bases:

>100_bases
AATGTATTACGCGAATGTTCGCCTTCTTTTTAAAGTAAGAAGATAGTGGCAGACGAAAAAGTATCAGATTAATTGAACGT
ATGAGCAAAAAGGGACCAGC

Downstream 100 bases:

>100_bases
TTTAATTGTCATAAGCCAATATTACAAAAACCCGCCAAAGATAAACGGTGGGTTTACAAGGCCACAGAGCCTTTTAACTG
AGCATACTAAATGTCAGAAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSEVQLRNNWKREEIESLFALPMNDLLFQAHSIHRQEFDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMVEEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTS
PEYYGDVITTRTYQSRLDTLSNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLDQHPDSVPINMLVKVEGTPFEKLDD
LDPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMGLFRRLGLHPEQGVA
STKEQDEAMLAKAAAQQDKKVSAFYDAGAL

Sequences:

>Translated_350_residues
MSEVQLRNNWKREEIESLFALPMNDLLFQAHSIHRQEFDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMVEEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTS
PEYYGDVITTRTYQSRLDTLSNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLDQHPDSVPINMLVKVEGTPFEKLDD
LDPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMGLFRRLGLHPEQGVA
STKEQDEAMLAKAAAQQDKKVSAFYDAGAL
>Mature_349_residues
SEVQLRNNWKREEIESLFALPMNDLLFQAHSIHRQEFDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLAM
ETVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMVEEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTSP
EYYGDVITTRTYQSRLDTLSNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLDQHPDSVPINMLVKVEGTPFEKLDDL
DPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMGLFRRLGLHPEQGVAS
TKEQDEAMLAKAAAQQDKKVSAFYDAGAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=345, Percent_Identity=65.2173913043478, Blast_Score=478, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=47.4683544303797, Blast_Score=311, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_SHEHH (B0TJN8)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674693.1
- ProteinModelPortal:   B0TJN8
- SMR:   B0TJN8
- GeneID:   5905856
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2477
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2304484
- BioCyc:   SHAL458817:SHAL_2477-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 38876; Mature: 38744

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVQLRNNWKREEIESLFALPMNDLLFQAHSIHRQEFDPNEVQISRLLSIKTGACPEDC
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
EEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTSPEYYGDVITTRTYQSRLDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
SNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLDQHPDSVPINMLVKVEGTPFEKLDD
HHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHCC
LDPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ENDDMGLFRRLGLHPEQGVASTKEQDEAMLAKAAAQQDKKVSAFYDAGAL
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure 
SEVQLRNNWKREEIESLFALPMNDLLFQAHSIHRQEFDPNEVQISRLLSIKTGACPEDC
CCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
EEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTSPEYYGDVITTRTYQSRLDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
SNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLDQHPDSVPINMLVKVEGTPFEKLDD
HHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHCC
LDPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ENDDMGLFRRLGLHPEQGVASTKEQDEAMLAKAAAQQDKKVSAFYDAGAL
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA