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The map label for this gene is pbuE [H]

Identifier: 167624345

GI number: 167624345

Start: 2923193

End: 2924374

Strand: Reverse

Name: pbuE [H]

Synonym: Shal_2422

Alternate gene names: 167624345

Gene position: 2924374-2923193 (Counterclockwise)

Preceding gene: 167624346

Following gene: 167624337

Centisome position: 55.95

GC content: 48.14

Gene sequence:

>1182_bases
ATGAACCCCAAAGTATACCTCTTAGCGCTGATCACTTTTGCTACGGGAATGGATGAAAATATCGCTGGTGGTATCGTCCC
CTTTATTGCTGAAGATCTTGGTGTTTCGATCAGTGCTGCAGGCCAGCTCACTACCGTATTTTCACTTACTTTCGCCTTGG
CCGCCCCCGTTTTACTGTTTGCGACCGCAAAGTTTAATCGCCTTCGCCTTTTACAGGTCGCGCTGTTCACCTTCTGTTTA
GGTAACCTATTCTCAGCGCTCAGCCCAAGCTACGCCTTTCTTTTTGGTATGCGTATTATCTCAGCGGCAAGCTGCGCACT
TATCGTTGTACTGGTGACCACCATAGCCACCGAAATGGTTGAAGCTGAGTTTAAAGGCCGAGCGATCGGCATCATCTTTA
TGGGCATTAGCGGCTCGTTAGTATTGGGGGTTCCGGTAGGCTTACTTTTTTGTAACTGGTTAGGCTGGCGCGCTCCCTTT
GCTTTTATCGCCCTTATGGCGCTATTACTCTGCATTATTGTGCCATTGATGTTACCCAATGTTTCTAGCAATGGGCAACG
CATTCCGCTCTCAAAATATGCAGAACACCTTAAAAACCCGAAACTTCTTACTGCCCAGCTAGTATCGATCTTGATGATCG
GCGGCCACTTTGTTCTTTTTGCCTATCTGGCCCCCTACCTACAGGCCACTATCGGTGCCAGTAAATCAGAGCTGACCTTC
TACTATTTTCTATTCGGAGCCGCAGCGATCACTGGCGGCTATATGGGTGGCTGGCTGTCAGACAAGCTAGGCCATATCAC
CGCTATCATACTCATACCGTCGATATTTATCCTATTTCTCGGTGCGATCCCCTTCACGCTTAATTCATTAGTACTGTTTA
TCCCCTGCATGATGCTCTGGGGTGGCTTAAGCTGGACGATATCTCCTATTGTGCAGAACTACCTGATCCAATCTGACCAC
GCCAATGCTGGTGTTAGTATCGCTGTCAACAACACCTCAATGCACCTTGGTGTAGCGCTTGGTTCACTACTCGGTGGCGT
AGTCATAAAGTACAGTTCTGTTAATTGGACTCCAGCAATAGGCGTGTTCGTGGTGGTGCTATCTCTGCTATGCGCACTCT
ACTCCACACGCTGTCAGGAAATCACGCGAGAAGAATGTAAGGAAAAGCTAATACAGCCATAA

Upstream 100 bases:

>100_bases
GTTAAATCATTCTATAAAACAAAAGTTTAGCCGAAACTGAGTTATACCAATAACGAAGTAAGCCATTAAGCTATTTTATT
AATATGACCACGAGTATGAC

Downstream 100 bases:

>100_bases
TTATTTTCTGTTATGCAGTAAAGCGGTTTACCACGAAGAATATTGAGGCAATGAAAGACACTGGGGCCACGAAGAAAAGC
CACAGCTTATATCGAACAAC

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNPKVYLLALITFATGMDENIAGGIVPFIAEDLGVSISAAGQLTTVFSLTFALAAPVLLFATAKFNRLRLLQVALFTFCL
GNLFSALSPSYAFLFGMRIISAASCALIVVLVTTIATEMVEAEFKGRAIGIIFMGISGSLVLGVPVGLLFCNWLGWRAPF
AFIALMALLLCIIVPLMLPNVSSNGQRIPLSKYAEHLKNPKLLTAQLVSILMIGGHFVLFAYLAPYLQATIGASKSELTF
YYFLFGAAAITGGYMGGWLSDKLGHITAIILIPSIFILFLGAIPFTLNSLVLFIPCMMLWGGLSWTISPIVQNYLIQSDH
ANAGVSIAVNNTSMHLGVALGSLLGGVVIKYSSVNWTPAIGVFVVVLSLLCALYSTRCQEITREECKEKLIQP

Sequences:

>Translated_393_residues
MNPKVYLLALITFATGMDENIAGGIVPFIAEDLGVSISAAGQLTTVFSLTFALAAPVLLFATAKFNRLRLLQVALFTFCL
GNLFSALSPSYAFLFGMRIISAASCALIVVLVTTIATEMVEAEFKGRAIGIIFMGISGSLVLGVPVGLLFCNWLGWRAPF
AFIALMALLLCIIVPLMLPNVSSNGQRIPLSKYAEHLKNPKLLTAQLVSILMIGGHFVLFAYLAPYLQATIGASKSELTF
YYFLFGAAAITGGYMGGWLSDKLGHITAIILIPSIFILFLGAIPFTLNSLVLFIPCMMLWGGLSWTISPIVQNYLIQSDH
ANAGVSIAVNNTSMHLGVALGSLLGGVVIKYSSVNWTPAIGVFVVVLSLLCALYSTRCQEITREECKEKLIQP
>Mature_393_residues
MNPKVYLLALITFATGMDENIAGGIVPFIAEDLGVSISAAGQLTTVFSLTFALAAPVLLFATAKFNRLRLLQVALFTFCL
GNLFSALSPSYAFLFGMRIISAASCALIVVLVTTIATEMVEAEFKGRAIGIIFMGISGSLVLGVPVGLLFCNWLGWRAPF
AFIALMALLLCIIVPLMLPNVSSNGQRIPLSKYAEHLKNPKLLTAQLVSILMIGGHFVLFAYLAPYLQATIGASKSELTF
YYFLFGAAAITGGYMGGWLSDKLGHITAIILIPSIFILFLGAIPFTLNSLVLFIPCMMLWGGLSWTISPIVQNYLIQSDH
ANAGVSIAVNNTSMHLGVALGSLLGGVVIKYSSVNWTPAIGVFVVVLSLLCALYSTRCQEITREECKEKLIQP

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=378, Percent_Identity=28.8359788359788, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786595, Length=389, Percent_Identity=23.6503856041131, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787808, Length=237, Percent_Identity=25.7383966244726, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42194; Mature: 42194

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKVYLLALITFATGMDENIAGGIVPFIAEDLGVSISAAGQLTTVFSLTFALAAPVLLF
CCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHH
ATAKFNRLRLLQVALFTFCLGNLFSALSPSYAFLFGMRIISAASCALIVVLVTTIATEMV
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAEFKGRAIGIIFMGISGSLVLGVPVGLLFCNWLGWRAPFAFIALMALLLCIIVPLMLPN
HHHHCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VSSNGQRIPLSKYAEHLKNPKLLTAQLVSILMIGGHFVLFAYLAPYLQATIGASKSELTF
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
YYFLFGAAAITGGYMGGWLSDKLGHITAIILIPSIFILFLGAIPFTLNSLVLFIPCMMLW
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGLSWTISPIVQNYLIQSDHANAGVSIAVNNTSMHLGVALGSLLGGVVIKYSSVNWTPAI
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEECCCCCCHHH
GVFVVVLSLLCALYSTRCQEITREECKEKLIQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNPKVYLLALITFATGMDENIAGGIVPFIAEDLGVSISAAGQLTTVFSLTFALAAPVLLF
CCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHH
ATAKFNRLRLLQVALFTFCLGNLFSALSPSYAFLFGMRIISAASCALIVVLVTTIATEMV
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAEFKGRAIGIIFMGISGSLVLGVPVGLLFCNWLGWRAPFAFIALMALLLCIIVPLMLPN
HHHHCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VSSNGQRIPLSKYAEHLKNPKLLTAQLVSILMIGGHFVLFAYLAPYLQATIGASKSELTF
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
YYFLFGAAAITGGYMGGWLSDKLGHITAIILIPSIFILFLGAIPFTLNSLVLFIPCMMLW
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGLSWTISPIVQNYLIQSDHANAGVSIAVNNTSMHLGVALGSLLGGVVIKYSSVNWTPAI
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEECCCCCCHHH
GVFVVVLSLLCALYSTRCQEITREECKEKLIQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA