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The map label for this gene is rimK

Identifier: 167623998

GI number: 167623998

Start: 2496871

End: 2498247

Strand: Direct

Name: rimK

Synonym: Shal_2072

Alternate gene names: 167623998

Gene position: 2496871-2498247 (Clockwise)

Preceding gene: 167623997

Following gene: 167623999

Centisome position: 47.77

GC content: 44.73

Gene sequence:

>1377_bases
ATGAGCGACAACAAGTTTATTATCGGAAGTGAAGAGTGGTGTGCATTCCCAGCTCTTGGTGTTCCAGCAATTAAAGCTAG
AGTTGACTCTGGCGCCAGAACGTCATCAATCCATGCTGTCAATATCCGCCCTTTTAAAAGAGAAGGCGAGCCATGGGTTT
CTTTTGAACTTCACCCGATACAAAATAGTCGCAAGATCATCCTTCGATGTGAAAGTAAAGTTATCGACCGTCGCAACATT
AAGAGCTCTAACGGTGAGATCGAAAAACGCTACGTTATCGCCTCTGTTATTCAGTTAGGCGGCCACGCTTGGGAAGTTGA
GCTAACCTTGACTAACCGCGACAGTATGGGATATCGCATGTTGTTAGGTCGTGAAGCGATGAGCAACAAGACCTTGGTTG
ACCCATCGGAATCATTTTGCTTAGGTCAGCAGAACGACGTTGAGGTTGAACAACTTTACGGTGTGAAATCAGCTAAGAAA
AGTGGTCTTAAGATCGGTTTGTTAGCAAGTAACCCTGACTTGTACAGTAACCGACGCATCATTGAAGCGGGTGAACAACG
TGGTCATGAGATGGTGTTTTTAAACATCAAACAGTGTTACTTAAAGCTCGATGCCTCTGAGCCTGAGGTACATTACCGTG
GCGGCCGCATCTTAAATGATCTTGATGCAGTCATTCCACGCATCAGACCAAGTATGACCTTCTACGGTTGCGCGTTAACG
CGTCACTTTGAAAGTTTGAATATCATGGCGCTTAACACCTCTGATGCGATTACACGCTCAAGAGATAAACTATTCTCGTT
GCAGTTACTGCAAAAGAATAACTTAGATATTCCAACAAGTGGCTTTGCTAATTCACCCGTTGATACGAAAGATCTTATCG
ATATGGTTGGTGGTGCACCACTTATCGTTAAGCTACTTGAGGGAACTCAAGGTAAAGGTGTTGTATTAGCCGAAACCACC
AAAGCGGCTGAAAGCGTAATCAATGCTTTCAAATCGCTACATGTAAATCTACTTGTGCAAGAATTCATTAAAGAAGCCCA
GGGTAAGGATTTACGCTGTTTTGTTATTGACGGCAAGGTTGTTGCTTCTATTGAGCGTACAGCGGCTCCTGGAGAGTTTA
GAGCTAACATCCATCAAGGTGGTAGTGCGTCTATTGTCTCGGTAAGTGCTGCAGAGCGACAATTGGCTCTGAAAGCTGCC
AAGACAATGGGTCTTCGTGTTGCTGGTGTTGATATCATTCGCTCTAGTCGCGGACCATTATTGCTTGAGATTAACTCATC
TCCAGGGCTTGAAGGTATCGAATCTGCGACAGGTATCGATATCGCTGGTGCGATGATCGATTCTATTGAGAAGAAGTTGG
GTTGGAAGGCTGAGTAA

Upstream 100 bases:

>100_bases
ATCCTATCCGTTTGTAGAAAATAACTAAAATTTAGTTTGCATCTGCTGAAAAAAGGAGTACTTTCGCGCTCCTTAATTTA
ACTTAACTGGAAGAATTGTA

Downstream 100 bases:

>100_bases
TCCGCTCCAACCTAGGATAAATGAAAAAGCGTTATGCCTCTGGCATAGCGCTTTTTTTATAGCCTAGTGATTATATTTTC
TTTAGTCATGAGATAAGTTT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPIQNSRKIILRCESKVIDRRNI
KSSNGEIEKRYVIASVIQLGGHAWEVELTLTNRDSMGYRMLLGREAMSNKTLVDPSESFCLGQQNDVEVEQLYGVKSAKK
SGLKIGLLASNPDLYSNRRIIEAGEQRGHEMVFLNIKQCYLKLDASEPEVHYRGGRILNDLDAVIPRIRPSMTFYGCALT
RHFESLNIMALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAPLIVKLLEGTQGKGVVLAETT
KAAESVINAFKSLHVNLLVQEFIKEAQGKDLRCFVIDGKVVASIERTAAPGEFRANIHQGGSASIVSVSAAERQLALKAA
KTMGLRVAGVDIIRSSRGPLLLEINSSPGLEGIESATGIDIAGAMIDSIEKKLGWKAE

Sequences:

>Translated_458_residues
MSDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPIQNSRKIILRCESKVIDRRNI
KSSNGEIEKRYVIASVIQLGGHAWEVELTLTNRDSMGYRMLLGREAMSNKTLVDPSESFCLGQQNDVEVEQLYGVKSAKK
SGLKIGLLASNPDLYSNRRIIEAGEQRGHEMVFLNIKQCYLKLDASEPEVHYRGGRILNDLDAVIPRIRPSMTFYGCALT
RHFESLNIMALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAPLIVKLLEGTQGKGVVLAETT
KAAESVINAFKSLHVNLLVQEFIKEAQGKDLRCFVIDGKVVASIERTAAPGEFRANIHQGGSASIVSVSAAERQLALKAA
KTMGLRVAGVDIIRSSRGPLLLEINSSPGLEGIESATGIDIAGAMIDSIEKKLGWKAE
>Mature_457_residues
SDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPIQNSRKIILRCESKVIDRRNIK
SSNGEIEKRYVIASVIQLGGHAWEVELTLTNRDSMGYRMLLGREAMSNKTLVDPSESFCLGQQNDVEVEQLYGVKSAKKS
GLKIGLLASNPDLYSNRRIIEAGEQRGHEMVFLNIKQCYLKLDASEPEVHYRGGRILNDLDAVIPRIRPSMTFYGCALTR
HFESLNIMALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAPLIVKLLEGTQGKGVVLAETTK
AAESVINAFKSLHVNLLVQEFIKEAQGKDLRCFVIDGKVVASIERTAAPGEFRANIHQGGSASIVSVSAAERQLALKAAK
TMGLRVAGVDIIRSSRGPLLLEINSSPGLEGIESATGIDIAGAMIDSIEKKLGWKAE

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=210, Percent_Identity=26.1904761904762, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI134254462, Length=210, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1787076, Length=289, Percent_Identity=57.439446366782, Blast_Score=339, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK_SHEHH (B0TTR5)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674292.1
- ProteinModelPortal:   B0TTR5
- SMR:   B0TTR5
- GeneID:   5904846
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2072
- HOGENOM:   HBG679591
- OMA:   CREPRLY
- ProtClustDB:   CLSK922420
- BioCyc:   SHAL458817:SHAL_2072-MONOMER
- GO:   GO:0006508
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008503
- InterPro:   IPR009007
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:2.40.70.10
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF05618 DUF785; PF08443 RimK

EC number: NA

Molecular weight: Translated: 50125; Mature: 49994

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPI
CCCCEEEEECCCCCCCCCCCCCHHEEECCCCCCCCEEEEEEEEEECCCCCCEEEEEEEEC
QNSRKIILRCESKVIDRRNIKSSNGEIEKRYVIASVIQLGGHAWEVELTLTNRDSMGYRM
CCCCEEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCEEE
LLGREAMSNKTLVDPSESFCLGQQNDVEVEQLYGVKSAKKSGLKIGLLASNPDLYSNRRI
EECCHHHCCCEEECCCCCCCCCCCCCCCHHHHHCCHHHHCCCCEEEEEECCCCCCCCCEE
IEAGEQRGHEMVFLNIKQCYLKLDASEPEVHYRGGRILNDLDAVIPRIRPSMTFYGCALT
EECHHHCCCEEEEEEEEEEEEEECCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEHHHHH
RHFESLNIMALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAP
HHHCCEEEEEECCCHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
LIVKLLEGTQGKGVVLAETTKAAESVINAFKSLHVNLLVQEFIKEAQGKDLRCFVIDGKV
EEHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCE
VASIERTAAPGEFRANIHQGGSASIVSVSAAERQLALKAAKTMGLRVAGVDIIRSSRGPL
EEEEHHCCCCCHHEEEECCCCCCEEEEECHHHHHHHHHHHHHCCEEEEEEEEEECCCCCE
LLEINSSPGLEGIESATGIDIAGAMIDSIEKKLGWKAE
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPI
CCCEEEEECCCCCCCCCCCCCHHEEECCCCCCCCEEEEEEEEEECCCCCCEEEEEEEEC
QNSRKIILRCESKVIDRRNIKSSNGEIEKRYVIASVIQLGGHAWEVELTLTNRDSMGYRM
CCCCEEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCEEE
LLGREAMSNKTLVDPSESFCLGQQNDVEVEQLYGVKSAKKSGLKIGLLASNPDLYSNRRI
EECCHHHCCCEEECCCCCCCCCCCCCCCHHHHHCCHHHHCCCCEEEEEECCCCCCCCCEE
IEAGEQRGHEMVFLNIKQCYLKLDASEPEVHYRGGRILNDLDAVIPRIRPSMTFYGCALT
EECHHHCCCEEEEEEEEEEEEEECCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEHHHHH
RHFESLNIMALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAP
HHHCCEEEEEECCCHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
LIVKLLEGTQGKGVVLAETTKAAESVINAFKSLHVNLLVQEFIKEAQGKDLRCFVIDGKV
EEHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCE
VASIERTAAPGEFRANIHQGGSASIVSVSAAERQLALKAAKTMGLRVAGVDIIRSSRGPL
EEEEHHCCCCCHHEEEECCCCCCEEEEECHHHHHHHHHHHHHCCEEEEEEEEEECCCCCE
LLEINSSPGLEGIESATGIDIAGAMIDSIEKKLGWKAE
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA