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The map label for this gene is eda [H]

Identifier: 167623882

GI number: 167623882

Start: 2346822

End: 2347469

Strand: Direct

Name: eda [H]

Synonym: Shal_1952

Alternate gene names: 167623882

Gene position: 2346822-2347469 (Clockwise)

Preceding gene: 167623881

Following gene: 167623884

Centisome position: 44.9

GC content: 47.69

Gene sequence:

>648_bases
ATGCTTGAGAATAACTGGTCAATACAGCCACAAGATATTTTTAAACGCAGCCCTATTGTTCCTGTCATGGTGATCAATAA
GATTGAACATGCTGTACCGTTAGCCAAGGCGCTAGTTGCCGGGGGTATTTCAGTACTTGAAGTCACTCTACGTACCGACT
GTGCGTTAGAGGCGATCACTAAGATTGCTCAAGAAGTACCAGAAGCAATAGTGGGAGCGGGAACAATTCTAAACAAGACG
CAGTTAGAGCAAGCCGTTCAAGCTGGCGCGCAGTTTGTGATCACCCCTGGTGCGACTACTGAACTGTTAGAGGCTGCAAT
GGCGGGCACAACACCGCTGATCCCTGGCGTTGCCAGCATCTCTGAAGTGATGAAAGGGATGGCACTGGGTTATACAAACT
TTAAGTTTTTCCCAGCTGAAGCCTCTGGTGGTGTTAATGCACTTAAAGCGTTTTCGGGTCCATTAGCCGACATTCGTTTC
TGCCCAACGGGTGGCATTACTCCAAGTAACTATAAAGAGTACTTAGCGCTTAAGAATGTCGACTGTATTGGGGGAAGCTG
GATTGCGCCTACTGATGCAATGGAGCAAGGTGACTGGGAAAGGATCACCGCCTTATGTAAAGAAGCCATCGATGCGCTTA
AAGTGTAA

Upstream 100 bases:

>100_bases
ATTCGGTGCACTGCGCGCTAACCTAAGTAGTCCTGAAACTGGTGCCCGTTGCACTAGCGCAATTGATGAAAACTATTAAC
GTTTAAGGAAGAGTAACGCA

Downstream 100 bases:

>100_bases
ATGATTCTTTCTAATAAAAAAGCTACCCTTGGGTAGCTTTTTTTATGTCCATTATTGGATTAGTTTCATCTCATCAAATT
ACAAGCGGTGCTATTTTGTA

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MLENNWSIQPQDIFKRSPIVPVMVINKIEHAVPLAKALVAGGISVLEVTLRTDCALEAITKIAQEVPEAIVGAGTILNKT
QLEQAVQAGAQFVITPGATTELLEAAMAGTTPLIPGVASISEVMKGMALGYTNFKFFPAEASGGVNALKAFSGPLADIRF
CPTGGITPSNYKEYLALKNVDCIGGSWIAPTDAMEQGDWERITALCKEAIDALKV

Sequences:

>Translated_215_residues
MLENNWSIQPQDIFKRSPIVPVMVINKIEHAVPLAKALVAGGISVLEVTLRTDCALEAITKIAQEVPEAIVGAGTILNKT
QLEQAVQAGAQFVITPGATTELLEAAMAGTTPLIPGVASISEVMKGMALGYTNFKFFPAEASGGVNALKAFSGPLADIRF
CPTGGITPSNYKEYLALKNVDCIGGSWIAPTDAMEQGDWERITALCKEAIDALKV
>Mature_215_residues
MLENNWSIQPQDIFKRSPIVPVMVINKIEHAVPLAKALVAGGISVLEVTLRTDCALEAITKIAQEVPEAIVGAGTILNKT
QLEQAVQAGAQFVITPGATTELLEAAMAGTTPLIPGVASISEVMKGMALGYTNFKFFPAEASGGVNALKAFSGPLADIRF
CPTGGITPSNYKEYLALKNVDCIGGSWIAPTDAMEQGDWERITALCKEAIDALKV

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=212, Percent_Identity=60.8490566037736, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI48994954, Length=170, Percent_Identity=25.8823529411765, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22701; Mature: 22701

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENNWSIQPQDIFKRSPIVPVMVINKIEHAVPLAKALVAGGISVLEVTLRTDCALEAIT
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KIAQEVPEAIVGAGTILNKTQLEQAVQAGAQFVITPGATTELLEAAMAGTTPLIPGVASI
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHH
SEVMKGMALGYTNFKFFPAEASGGVNALKAFSGPLADIRFCPTGGITPSNYKEYLALKNV
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHCCC
DCIGGSWIAPTDAMEQGDWERITALCKEAIDALKV
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLENNWSIQPQDIFKRSPIVPVMVINKIEHAVPLAKALVAGGISVLEVTLRTDCALEAIT
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KIAQEVPEAIVGAGTILNKTQLEQAVQAGAQFVITPGATTELLEAAMAGTTPLIPGVASI
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHH
SEVMKGMALGYTNFKFFPAEASGGVNALKAFSGPLADIRFCPTGGITPSNYKEYLALKNV
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHCCC
DCIGGSWIAPTDAMEQGDWERITALCKEAIDALKV
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]