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The map label for this gene is thiH [H]

Identifier: 167623835

GI number: 167623835

Start: 2288551

End: 2289657

Strand: Direct

Name: thiH [H]

Synonym: Shal_1905

Alternate gene names: 167623835

Gene position: 2288551-2289657 (Clockwise)

Preceding gene: 167623834

Following gene: 167623836

Centisome position: 43.78

GC content: 46.97

Gene sequence:

>1107_bases
ATGAGCTTTGTCGAAGTATTTAAAAAGCTATCACGCTCTGAGCTTAAGTTAAGGCTGTATTCCTGCACGGCAGCCGATGT
GGAGCAGGCATTACAGGGGGCATCGGGTAATTTAGATAGTTTACTTGCGCTCCTGTCTCCAGCTGCTCAGCCCTATCTTG
AGCAGATGGCGCAGCAGGCTGTTACGTTAACGCGTCAGCGCTTTGGTGCCAGTATCGGCATGTATATTCCGTTATACCTA
TCGAATCTATGCGCCAATGAATGCGATTACTGTGGCTTTACCATGAGCAATAAAATTAAGCGTAAGACCTTGTCCAATGA
TGAGGTGTTGGATGAGATGCGCTTAATTAAAACTATGGGTTACGACTCTATTTTACTGGTATCAGGTGAGCACGAAACTA
AGGTGGGTATAGATTACTTTAAACAAGTCTTGCCCTTGGTTCGAGAGCAATTTAGCCATGTGGCCATGGAAGTACAGCCT
CTTGGAGAGCAAGATTATCGAGAGTTAGTGGCCAGTGGGCTCGATGCGGTCATGTTGTATCAAGAAACCTATAACCCCGA
GACTTATAGCAAACACCATACGCGAGGCAAAAAGAAAGATTTTATCTTTCGGCTCGAGTCCCCCGACAGAGTCGCGAGAG
CCGGTGTCGATAAGATTGGCTTGGGCGTATTGTTAGGTTTGGATGACTGGCGACTAGACGCGCTGTTAATGGGTCATCAT
ATTGATTATTTAGAAAAAACTTACTGGCGTAGTCGCTATAGTATTTCTCTGCCAAGACTTAGGCCCTGTACGGGCGGGAT
ATCGCCAAAAGTGGAGCTAACCGATAAAGGCCTAGTGCAGATGATCTGCGCCTTTAGGTTGTTTAATCAACAGTTAGAGA
TTAGTCTATCGACACGAGAAACCCCGAAACTTCGAGATAATTTGTTTGCCCTAGGAGTAACCAATGTTAGCGCTGGCAGT
TCAACTCAGCCAGGCGGTTATGTCGAGCCCAATACCGAACTGGATCAATTTGAGATCAGTGACGGGCGAACGCCGCAAGT
GGTCGCCAAAGCGATGCTTGAGCGAGGGTTAAATCCGGTTTGGAAAGACTGGGAAACTGGGTGGTAG

Upstream 100 bases:

>100_bases
TGTGGCAGGGCTTGGTGCTGTTAGCCATCAAGCGCACAATACCAGTCCGCTTACGGGGTTCTTAAATGATGAGTAATTTT
TCAGCCTCGAAGGAGGGGTT

Downstream 100 bases:

>100_bases
CTCTGGTATTTAGGTTAATAGTGTGAGTTTCGCTAAAGTAAAATCAATCGAATGGATGCATTGATAATATGTTTAAACAG
GGAAAAGCTATGGATGTGAA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSFVEVFKKLSRSELKLRLYSCTAADVEQALQGASGNLDSLLALLSPAAQPYLEQMAQQAVTLTRQRFGASIGMYIPLYL
SNLCANECDYCGFTMSNKIKRKTLSNDEVLDEMRLIKTMGYDSILLVSGEHETKVGIDYFKQVLPLVREQFSHVAMEVQP
LGEQDYRELVASGLDAVMLYQETYNPETYSKHHTRGKKKDFIFRLESPDRVARAGVDKIGLGVLLGLDDWRLDALLMGHH
IDYLEKTYWRSRYSISLPRLRPCTGGISPKVELTDKGLVQMICAFRLFNQQLEISLSTRETPKLRDNLFALGVTNVSAGS
STQPGGYVEPNTELDQFEISDGRTPQVVAKAMLERGLNPVWKDWETGW

Sequences:

>Translated_368_residues
MSFVEVFKKLSRSELKLRLYSCTAADVEQALQGASGNLDSLLALLSPAAQPYLEQMAQQAVTLTRQRFGASIGMYIPLYL
SNLCANECDYCGFTMSNKIKRKTLSNDEVLDEMRLIKTMGYDSILLVSGEHETKVGIDYFKQVLPLVREQFSHVAMEVQP
LGEQDYRELVASGLDAVMLYQETYNPETYSKHHTRGKKKDFIFRLESPDRVARAGVDKIGLGVLLGLDDWRLDALLMGHH
IDYLEKTYWRSRYSISLPRLRPCTGGISPKVELTDKGLVQMICAFRLFNQQLEISLSTRETPKLRDNLFALGVTNVSAGS
STQPGGYVEPNTELDQFEISDGRTPQVVAKAMLERGLNPVWKDWETGW
>Mature_367_residues
SFVEVFKKLSRSELKLRLYSCTAADVEQALQGASGNLDSLLALLSPAAQPYLEQMAQQAVTLTRQRFGASIGMYIPLYLS
NLCANECDYCGFTMSNKIKRKTLSNDEVLDEMRLIKTMGYDSILLVSGEHETKVGIDYFKQVLPLVREQFSHVAMEVQPL
GEQDYRELVASGLDAVMLYQETYNPETYSKHHTRGKKKDFIFRLESPDRVARAGVDKIGLGVLLGLDDWRLDALLMGHHI
DYLEKTYWRSRYSISLPRLRPCTGGISPKVELTDKGLVQMICAFRLFNQQLEISLSTRETPKLRDNLFALGVTNVSAGSS
TQPGGYVEPNTELDQFEISDGRTPQVVAKAMLERGLNPVWKDWETGW

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=366, Percent_Identity=55.1912568306011, Blast_Score=446, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41526; Mature: 41395

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVEVFKKLSRSELKLRLYSCTAADVEQALQGASGNLDSLLALLSPAAQPYLEQMAQQA
CCHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
VTLTRQRFGASIGMYIPLYLSNLCANECDYCGFTMSNKIKRKTLSNDEVLDEMRLIKTMG
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCC
YDSILLVSGEHETKVGIDYFKQVLPLVREQFSHVAMEVQPLGEQDYRELVASGLDAVMLY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCEEEEE
QETYNPETYSKHHTRGKKKDFIFRLESPDRVARAGVDKIGLGVLLGLDDWRLDALLMGHH
HHCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHCCCHHHCCEEEECCCCHHHHHHHHHHH
IDYLEKTYWRSRYSISLPRLRPCTGGISPKVELTDKGLVQMICAFRLFNQQLEISLSTRE
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCHHHEEEECCCC
TPKLRDNLFALGVTNVSAGSSTQPGGYVEPNTELDQFEISDGRTPQVVAKAMLERGLNPV
CCHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCHH
WKDWETGW
HCCCCCCC
>Mature Secondary Structure 
SFVEVFKKLSRSELKLRLYSCTAADVEQALQGASGNLDSLLALLSPAAQPYLEQMAQQA
CHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
VTLTRQRFGASIGMYIPLYLSNLCANECDYCGFTMSNKIKRKTLSNDEVLDEMRLIKTMG
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCC
YDSILLVSGEHETKVGIDYFKQVLPLVREQFSHVAMEVQPLGEQDYRELVASGLDAVMLY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCEEEEE
QETYNPETYSKHHTRGKKKDFIFRLESPDRVARAGVDKIGLGVLLGLDDWRLDALLMGHH
HHCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHCCCHHHCCEEEECCCCHHHHHHHHHHH
IDYLEKTYWRSRYSISLPRLRPCTGGISPKVELTDKGLVQMICAFRLFNQQLEISLSTRE
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCHHHEEEECCCC
TPKLRDNLFALGVTNVSAGSSTQPGGYVEPNTELDQFEISDGRTPQVVAKAMLERGLNPV
CCHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCHH
WKDWETGW
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]