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The map label for this gene is thiG

Identifier: 167623834

GI number: 167623834

Start: 2287759

End: 2288526

Strand: Direct

Name: thiG

Synonym: Shal_1904

Alternate gene names: 167623834

Gene position: 2287759-2288526 (Clockwise)

Preceding gene: 167623833

Following gene: 167623835

Centisome position: 43.77

GC content: 51.17

Gene sequence:

>768_bases
ATGTTAACTATTGCACAGCACCAATTTGAATCACGATTATTTACCGGAACGGGCAAATTTGCCGCGTCGGAGATCATGCT
CACTGCCATTCAAGCATCGCAATCTCAACTTGTGACACTGGCGATTAAAAGGCTCGATCTTAAAACTGGTAGCGACAATA
TTCTCAAACCTTTGTTAGACAGAGGTGTAAAGCTTCTGCCTAACACTGCTGGGGCTCGCAACGTCAAAGAAGCCATTTTT
GCCGCCCATCTTTCACGAGAGATGTTAGGCACCAATTGGATTAAGCTCGAGATCCACCCGGATCCTAAGTACTTAATGCC
AGATCCTATCGAAACCTTAGAGGCGGCGCGGATTTTATGTGAGCAAGGTTATGTGGTCTTGCCCTATGTCCATGCCGACC
CTGTGCTGTGCCGCAGACTCGAAGAGGTGGGCTGCGCCGCAGTGATGCCACTTGGCAGCCCGATTGGCTCCAATCAAGGC
TTAGCCACAGAAACCTTTTTAAAGATTATTATTGAGCAAGCCAATGTACCTGTGATTGTCGATGCCGGGATAGGCGCACC
ATCTCAGGCTACCCACGCCATGGAGCTGGGCGCTGACGCGGTGTTGGTTAATACCGCGATTGCCAGCAGTTCAGATCCCG
TGGCTATGGGCCGCTGCTTTGCCCAAGCGGTCAACACGGGGCGGCAGGCATATGTGGCAGGGCTTGGTGCTGTTAGCCAT
CAAGCGCACAATACCAGTCCGCTTACGGGGTTCTTAAATGATGAGTAA

Upstream 100 bases:

>100_bases
GCACTTAACGCTGAGGTCGTGCCTCGTCAAAGCTGGCCGATTATCCATTGCCAAGCAGGCGATAAACTTGAACTCTTTTC
TGTCGTGGCCGGAGGCTAAA

Downstream 100 bases:

>100_bases
TTTTTCAGCCTCGAAGGAGGGGTTATGAGCTTTGTCGAAGTATTTAAAAAGCTATCACGCTCTGAGCTTAAGTTAAGGCT
GTATTCCTGCACGGCAGCCG

Product: thiazole biosynthesis family protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTIAQHQFESRLFTGTGKFAASEIMLTAIQASQSQLVTLAIKRLDLKTGSDNILKPLLDRGVKLLPNTAGARNVKEAIF
AAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILCEQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQG
LATETFLKIIIEQANVPVIVDAGIGAPSQATHAMELGADAVLVNTAIASSSDPVAMGRCFAQAVNTGRQAYVAGLGAVSH
QAHNTSPLTGFLNDE

Sequences:

>Translated_255_residues
MLTIAQHQFESRLFTGTGKFAASEIMLTAIQASQSQLVTLAIKRLDLKTGSDNILKPLLDRGVKLLPNTAGARNVKEAIF
AAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILCEQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQG
LATETFLKIIIEQANVPVIVDAGIGAPSQATHAMELGADAVLVNTAIASSSDPVAMGRCFAQAVNTGRQAYVAGLGAVSH
QAHNTSPLTGFLNDE
>Mature_255_residues
MLTIAQHQFESRLFTGTGKFAASEIMLTAIQASQSQLVTLAIKRLDLKTGSDNILKPLLDRGVKLLPNTAGARNVKEAIF
AAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILCEQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQG
LATETFLKIIIEQANVPVIVDAGIGAPSQATHAMELGADAVLVNTAIASSSDPVAMGRCFAQAVNTGRQAYVAGLGAVSH
QAHNTSPLTGFLNDE

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=66.798418972332, Blast_Score=346, Evalue=7e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_SHEHH (B0TS89)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674128.1
- ProteinModelPortal:   B0TS89
- SMR:   B0TS89
- GeneID:   5904708
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1904
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   CLSK981588
- BioCyc:   SHAL458817:SHAL_1904-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27147; Mature: 27147

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIAQHQFESRLFTGTGKFAASEIMLTAIQASQSQLVTLAIKRLDLKTGSDNILKPLLD
CCCCCHHHHHHHEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
RGVKLLPNTAGARNVKEAIFAAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILC
CCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
EQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQGLATETFLKIIIEQANVPVIV
HCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
DAGIGAPSQATHAMELGADAVLVNTAIASSSDPVAMGRCFAQAVNTGRQAYVAGLGAVSH
ECCCCCCCHHHHHHHHCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
QAHNTSPLTGFLNDE
CCCCCCCCEECCCCC
>Mature Secondary Structure
MLTIAQHQFESRLFTGTGKFAASEIMLTAIQASQSQLVTLAIKRLDLKTGSDNILKPLLD
CCCCCHHHHHHHEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
RGVKLLPNTAGARNVKEAIFAAHLSREMLGTNWIKLEIHPDPKYLMPDPIETLEAARILC
CCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
EQGYVVLPYVHADPVLCRRLEEVGCAAVMPLGSPIGSNQGLATETFLKIIIEQANVPVIV
HCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
DAGIGAPSQATHAMELGADAVLVNTAIASSSDPVAMGRCFAQAVNTGRQAYVAGLGAVSH
ECCCCCCCHHHHHHHHCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
QAHNTSPLTGFLNDE
CCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA