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The map label for this gene is hisH [H]

Identifier: 167623750

GI number: 167623750

Start: 2176287

End: 2177003

Strand: Reverse

Name: hisH [H]

Synonym: Shal_1820

Alternate gene names: 167623750

Gene position: 2177003-2176287 (Counterclockwise)

Preceding gene: 167623751

Following gene: 167623749

Centisome position: 41.65

GC content: 47.14

Gene sequence:

>717_bases
ATGAGCCAAACAGATATAGCAGCCATAGTCGGTAATACCGTCATTATCGATACTGGCTGCGCCAATTTAAGTTCTGTCCG
CTACGCCTTTGAACGCTTGATGACAGATATTGAGCGCGAAAACCTGTTTGTTAGCGATGACATTGAGCTAATTAAATCGG
CCGAGCGCGTCATTTTACCCGGTGTCGGTACCGCTGGCGCAGCAATGACTGCCCTCAAAGATAAAGGCTTGATAGAGGTG
ATTACTTCGCTCACCCAACCGGTACTCGGAGTGTGTTTAGGCATGCAGATGCTGACCCGCGCCTCGAAAGAGCGCGGTAC
ACAAAGCGATGACTGCCAATGCCTAAATATTATTGCAACGGATATCGAGCTACTCGATAGTCAGGGCTTACCTCTGCCCC
ATATGGGCTGGAATCAAATAACGCCTTCGAGTCACCCTATTTTTGATGGTATTACTGCCGGTAACTACGTTTACTTTGTG
CATAGCTACCGTGCGCCAATTAGCGACTACACCATTGCCACCGCCGAACATGGTGAGTGTTTTAGCGCGGCCATTGCAAA
AGATAATTTTATTGGACTGCAATTTCATCCGGAAAAAAGCGCTGAAGTTGGCGCGAAGATATTGGCAAACTTCCTCACTA
TGGACAGAAATACCTTTGGCCAATCAAGCACTCAACAAGCGAGTAAACTTGCTAAAAAGCAATTAGAGGCACTGTAA

Upstream 100 bases:

>100_bases
ACCACCACAAAGTTGAAAGTTTATATAAGGTGTTAGGTCGGACTCTAAGGCAAGCTGTTGCAGTTGAAGGAGATAGCCTT
CCCTCAAGTAAAGGTGTGTT

Downstream 100 bases:

>100_bases
TGATTATTCCTGCAATCGATCTTATCGACGGTCAAGTCGTTCGCCTCTACCAAGGTGATTACGAACAACAAACAACCTTT
AATTTGAGTCCGTTAGATCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSQTDIAAIVGNTVIIDTGCANLSSVRYAFERLMTDIERENLFVSDDIELIKSAERVILPGVGTAGAAMTALKDKGLIEV
ITSLTQPVLGVCLGMQMLTRASKERGTQSDDCQCLNIIATDIELLDSQGLPLPHMGWNQITPSSHPIFDGITAGNYVYFV
HSYRAPISDYTIATAEHGECFSAAIAKDNFIGLQFHPEKSAEVGAKILANFLTMDRNTFGQSSTQQASKLAKKQLEAL

Sequences:

>Translated_238_residues
MSQTDIAAIVGNTVIIDTGCANLSSVRYAFERLMTDIERENLFVSDDIELIKSAERVILPGVGTAGAAMTALKDKGLIEV
ITSLTQPVLGVCLGMQMLTRASKERGTQSDDCQCLNIIATDIELLDSQGLPLPHMGWNQITPSSHPIFDGITAGNYVYFV
HSYRAPISDYTIATAEHGECFSAAIAKDNFIGLQFHPEKSAEVGAKILANFLTMDRNTFGQSSTQQASKLAKKQLEAL
>Mature_237_residues
SQTDIAAIVGNTVIIDTGCANLSSVRYAFERLMTDIERENLFVSDDIELIKSAERVILPGVGTAGAAMTALKDKGLIEVI
TSLTQPVLGVCLGMQMLTRASKERGTQSDDCQCLNIIATDIELLDSQGLPLPHMGWNQITPSSHPIFDGITAGNYVYFVH
SYRAPISDYTIATAEHGECFSAAIAKDNFIGLQFHPEKSAEVGAKILANFLTMDRNTFGQSSTQQASKLAKKQLEAL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=49.0196078431373, Blast_Score=201, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=33.4905660377358, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 25746; Mature: 25614

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTDIAAIVGNTVIIDTGCANLSSVRYAFERLMTDIERENLFVSDDIELIKSAERVILP
CCCCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECCHHHHHHCCCCEEEC
GVGTAGAAMTALKDKGLIEVITSLTQPVLGVCLGMQMLTRASKERGTQSDDCQCLNIIAT
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DIELLDSQGLPLPHMGWNQITPSSHPIFDGITAGNYVYFVHSYRAPISDYTIATAEHGEC
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCH
FSAAIAKDNFIGLQFHPEKSAEVGAKILANFLTMDRNTFGQSSTQQASKLAKKQLEAL
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQTDIAAIVGNTVIIDTGCANLSSVRYAFERLMTDIERENLFVSDDIELIKSAERVILP
CCCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECCHHHHHHCCCCEEEC
GVGTAGAAMTALKDKGLIEVITSLTQPVLGVCLGMQMLTRASKERGTQSDDCQCLNIIAT
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DIELLDSQGLPLPHMGWNQITPSSHPIFDGITAGNYVYFVHSYRAPISDYTIATAEHGEC
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCH
FSAAIAKDNFIGLQFHPEKSAEVGAKILANFLTMDRNTFGQSSTQQASKLAKKQLEAL
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA