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The map label for this gene is hisF

Identifier: 167623748

GI number: 167623748

Start: 2174795

End: 2175568

Strand: Reverse

Name: hisF

Synonym: Shal_1818

Alternate gene names: 167623748

Gene position: 2175568-2174795 (Counterclockwise)

Preceding gene: 167623749

Following gene: 167623747

Centisome position: 41.62

GC content: 47.03

Gene sequence:

>774_bases
ATGTTGGCCAAACGCATAGTCCCTTGCCTCGATGTCAAAGAGGGTAAAGTGGTTAAAGGGGTTCAATTTAGAAACCATGA
AATTGTAGGTGATATTGTGCCGCTCGCTAAGCGCTACGCTGAAGAAGGTGCAGATGAATTAGTCTTCTATGACATCACCG
CCAGCGCACATGATAGGGTCATTGATAAATCTTGGATAAGCCGAGTCGCCGAAAAAATAGATATCCCTTTTTGTGTTGCA
GGTGGGATCAGAACCATAGAGCAAGCACGGGAAAAGCTGGCCTTTGGCGCAGATAAAATTTCAATTAATTCACCAGCATT
AACCGACCCTAGCTTGATTGCCAGACTGCAAGATGAATTTGGCCGCCAGTGCATCGTCATCGGTATCGACTCCTACTATG
ACGCTAGTAGCGACAGCTATAGGGTCAAGCAGTTTACTGGCGACGAAGCGGCGACCAAAGACACTCAGTGGTATACACAA
GACTGGGTTGAAGAGGTGCAAAAACAAGGCTGCGGCGAAATCGTACTCAACGTCATGAACCAAGATGGTGTCAGGCAAGG
TTATGACATTAAGCAACTGTCGATGATACGTAGCATCTGCGATGTGCCATTAATCGCATCGGGTGGCGCCGGCACCATGG
CGCACTTTAGGGATGTATTTAGCCGAGCCAATGTCGATGCGGCGCTCGCGGCAAGTGTATTTCATAAAGGGATCATCGAT
ATCCAAGAGTTAAAACAGTATCTTAAAGATAATCAGATTGCAGTACGCGTTTAG

Upstream 100 bases:

>100_bases
CCTTGGCCGATGTCAATGCAGTAAAAGATAGCGGCGCCGATGGCATCATCATCGGAAAAGCCTTACTCATTAAACAGTTT
TCAGTAAAGGAGGCGATTGC

Downstream 100 bases:

>100_bases
GCTAAATCTAAAAGATTTAATTAAACGAGTTTATCCAATTAGGGACATAATATGACTAAGCTTGACCAATCAAAGCCGGT
AGACAGCAACCTTATTGGTC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASAHDRVIDKSWISRVAEKIDIPFCVA
GGIRTIEQAREKLAFGADKISINSPALTDPSLIARLQDEFGRQCIVIGIDSYYDASSDSYRVKQFTGDEAATKDTQWYTQ
DWVEEVQKQGCGEIVLNVMNQDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMAHFRDVFSRANVDAALAASVFHKGIID
IQELKQYLKDNQIAVRV

Sequences:

>Translated_257_residues
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASAHDRVIDKSWISRVAEKIDIPFCVA
GGIRTIEQAREKLAFGADKISINSPALTDPSLIARLQDEFGRQCIVIGIDSYYDASSDSYRVKQFTGDEAATKDTQWYTQ
DWVEEVQKQGCGEIVLNVMNQDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMAHFRDVFSRANVDAALAASVFHKGIID
IQELKQYLKDNQIAVRV
>Mature_257_residues
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASAHDRVIDKSWISRVAEKIDIPFCVA
GGIRTIEQAREKLAFGADKISINSPALTDPSLIARLQDEFGRQCIVIGIDSYYDASSDSYRVKQFTGDEAATKDTQWYTQ
DWVEEVQKQGCGEIVLNVMNQDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMAHFRDVFSRANVDAALAASVFHKGIID
IQELKQYLKDNQIAVRV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=71.2062256809339, Blast_Score=390, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=27.7602523659306, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SHEHH (B0TQY8)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674042.1
- ProteinModelPortal:   B0TQY8
- SMR:   B0TQY8
- GeneID:   5904615
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1818
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- ProtClustDB:   PRK02083
- BioCyc:   SHAL458817:SHAL_1818-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28545; Mature: 28545

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASAHDRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
IDKSWISRVAEKIDIPFCVAGGIRTIEQAREKLAFGADKISINSPALTDPSLIARLQDEF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSYYDASSDSYRVKQFTGDEAATKDTQWYTQDWVEEVQKQGCGEIVLNVMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMAHFRDVFSRANVDAALAASVFHKGIID
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
IQELKQYLKDNQIAVRV
HHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MLAKRIVPCLDVKEGKVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASAHDRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
IDKSWISRVAEKIDIPFCVAGGIRTIEQAREKLAFGADKISINSPALTDPSLIARLQDEF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSYYDASSDSYRVKQFTGDEAATKDTQWYTQDWVEEVQKQGCGEIVLNVMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRQGYDIKQLSMIRSICDVPLIASGGAGTMAHFRDVFSRANVDAALAASVFHKGIID
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
IQELKQYLKDNQIAVRV
HHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA