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The map label for this gene is nfnB [C]

Identifier: 167623673

GI number: 167623673

Start: 2088845

End: 2089501

Strand: Reverse

Name: nfnB [C]

Synonym: Shal_1742

Alternate gene names: 167623673

Gene position: 2089501-2088845 (Counterclockwise)

Preceding gene: 167623677

Following gene: 167623671

Centisome position: 39.98

GC content: 42.62

Gene sequence:

>657_bases
ATGACTCACCCTATTATTGCCGATCTTGAAAGCCGTTACACTGCAAAACGCTATGATGCGTCAAAGCGTATTCCTCAGAC
CGATCTTGATGTGATCTATAAAGCAATTAACCTCTCGGCGTCATCTATCAATTCACAGCCTTGGAAATTTATTGTGATCG
AAAGTGACGAAGCTAAACAGCGTATGCACGATACGTTTGCCAACAAGTTTCAGTTTAACCAGCCACATATCAAGTCTGCA
TCGCATATTGTTTTATTTGCTTACAACCCTCGCTACACTCGCGAAGATTATGCAAAAGTCGTTGATAAAGGTATTGCCGA
TGGCCGTATGGGAGTTGAGGATAGAGAACAAGCATTCGGCGCTTTTGCTTTCGCTGAGTTAAATACCGATGAAACTGGTA
ATACATCAGCCTGGACCAAGGCACAAACCTATCTAGCCCTAGGTAACACATTGCATACCCTTGCGCGTCTTGGAATTGAC
TCTACCACCATGGAAGGTGTTGATAGTGAGTTGATTGGTGAAATATTTAAAGAAGAGCTTGGCGGTTATCAGTGTGATGT
TGCATTAGCCATGGGCTACCATCATTCTGAAGAAGATTACAATGCAGCACTACCTAAGTCTCGCTTAGCAATAGAAGATG
TACTGCAGGTTTTATAA

Upstream 100 bases:

>100_bases
TAATAATTCAACGAACAAACACTATTTCAATACTGAATATGGGGGTAGGATAGTGGTCAGTTTTATTATTGCCTCAACCT
TGCCACATTAGGATACGAAA

Downstream 100 bases:

>100_bases
CACAAGTCAGTTTTCATTAATCGAGTAAACTAGGTTTCTCGTTATGATAAGAGTCAAGTTTAGAGGCTCGGGGCTACAGC
CCCGAGCTTTTGTTTATAGT

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: FRASE I [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTHPIIADLESRYTAKRYDASKRIPQTDLDVIYKAINLSASSINSQPWKFIVIESDEAKQRMHDTFANKFQFNQPHIKSA
SHIVLFAYNPRYTREDYAKVVDKGIADGRMGVEDREQAFGAFAFAELNTDETGNTSAWTKAQTYLALGNTLHTLARLGID
STTMEGVDSELIGEIFKEELGGYQCDVALAMGYHHSEEDYNAALPKSRLAIEDVLQVL

Sequences:

>Translated_218_residues
MTHPIIADLESRYTAKRYDASKRIPQTDLDVIYKAINLSASSINSQPWKFIVIESDEAKQRMHDTFANKFQFNQPHIKSA
SHIVLFAYNPRYTREDYAKVVDKGIADGRMGVEDREQAFGAFAFAELNTDETGNTSAWTKAQTYLALGNTLHTLARLGID
STTMEGVDSELIGEIFKEELGGYQCDVALAMGYHHSEEDYNAALPKSRLAIEDVLQVL
>Mature_217_residues
THPIIADLESRYTAKRYDASKRIPQTDLDVIYKAINLSASSINSQPWKFIVIESDEAKQRMHDTFANKFQFNQPHIKSAS
HIVLFAYNPRYTREDYAKVVDKGIADGRMGVEDREQAFGAFAFAELNTDETGNTSAWTKAQTYLALGNTLHTLARLGIDS
TTMEGVDSELIGEIFKEELGGYQCDVALAMGYHHSEEDYNAALPKSRLAIEDVLQVL

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction. Major FMN reductase. It is capable of using both NADH and NADPH as electron donors. As electron acceptor, FMN is the most effectiv

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=207, Percent_Identity=35.7487922705314, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24474; Mature: 24342

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPIIADLESRYTAKRYDASKRIPQTDLDVIYKAINLSASSINSQPWKFIVIESDEAKQ
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCCCEEEEEEECCHHHH
RMHDTFANKFQFNQPHIKSASHIVLFAYNPRYTREDYAKVVDKGIADGRMGVEDREQAFG
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
AFAFAELNTDETGNTSAWTKAQTYLALGNTLHTLARLGIDSTTMEGVDSELIGEIFKEEL
HHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHH
GGYQCDVALAMGYHHSEEDYNAALPKSRLAIEDVLQVL
CCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
THPIIADLESRYTAKRYDASKRIPQTDLDVIYKAINLSASSINSQPWKFIVIESDEAKQ
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCCCEEEEEEECCHHHH
RMHDTFANKFQFNQPHIKSASHIVLFAYNPRYTREDYAKVVDKGIADGRMGVEDREQAFG
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
AFAFAELNTDETGNTSAWTKAQTYLALGNTLHTLARLGIDSTTMEGVDSELIGEIFKEEL
HHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHH
GGYQCDVALAMGYHHSEEDYNAALPKSRLAIEDVLQVL
CCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8206830; 8033996; 9654450 [H]