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The map label for this gene is trpB

Identifier: 167623585

GI number: 167623585

Start: 1983823

End: 1985082

Strand: Reverse

Name: trpB

Synonym: Shal_1654

Alternate gene names: 167623585

Gene position: 1985082-1983823 (Counterclockwise)

Preceding gene: 167623586

Following gene: 167623584

Centisome position: 37.98

GC content: 49.76

Gene sequence:

>1260_bases
ATGAGCAAGTTAAATCCCTACTTTGGGGAATACGGTGGTATGTACGTTCCACAAATCTTAATGCCAGCACTTAAGCAGCT
AGAGACCGCATTTATTGAAGCGCAAGAAGACGAAGCGTTTCAAGAAGAATTTACCGACCTATTAAAGAACTATGCGGGTC
GCCCAACGGCATTAACGCTAACCCGTAACTTAAGCCCTAACCCACTGGCTAAGATTTACTTAAAGCGTGAAGACCTACTT
CATGGTGGCGCCCACAAGACCAACCAAGTACTAGGTCAGGCGCTACTAGCAAAGCGCATGGGCAAGAAAGAGATCATTGC
CGAGACGGGCGCGGGTCAACACGGCGTGGCAACGGCACTTGCCTGTGCCCTGCTCGGTCTTAAATGCAGAGTATATATGG
GCGCCAAAGATGTTGAGCGTCAATCACCTAACGTATTTAGAATGAAGTTAATGGGCGCAGAAGTGATCCCGGTAACCTCA
GGCTCTGCCACCTTAAAAGATGCTTGTAACGAAGCGATGCGTGACTGGTCTGGTTGTTATGACAAGGCCCACTATCTACT
TGGCACCGCTGCGGGGCCTCATCCATATCCTACAATAGTACGCGAGTTCCAGCGCATGATTGGCGCTGAAACTAAGCGTC
AAATTCTTGAGCGTGAAGGTCGCCTGCCCGATGCCGTTATTGCCTGTGTTGGCGGTGGTTCAAATGCGATTGGTATGTTT
GCCGACTTTATCGATGAGCCAGAGGTTGCCTTGATTGGGGTTGAGCCTGCAGGTAAAGGCATTGATACGCCAATGCATGG
CGCGCCACTTCATCATGGTAAAACGGGGATTTTCTTCGGTATGAAAGCGCCACTGATGCAAGATAGCGAAGGTCAAGTCG
AAGAGTCTTACTCTGTATCGGCAGGCCTTGATTTCCCATCGGTTGGGCCGCAACATGCGCACCTTGCCGCCATTGGCCGC
GCAACTTACGAGTCGGCGACCGATGATGAAGCACTGGAAACCTTCCAACTGCTCGCCCGCTGTGAAGGGATTATTCCGGC
ACTGGAATCGGCTCACGCAATCGCTTATGCGGTGAAGTTAGCCAAAGAAGCCACCAAAGAGACCCTATTAGTGGTTAACC
TATCGGGCCGTGGTGACAAAGATATCTTCACCGTTGCGGACATTCTTGAGCAACAAAAAGTTGAGCAACAAAAAGTTGAG
CAACAAAAAGCTGACAATCAGAATACAGAGAAAAACAATCAGGAGAGTGGCAATGAGTAA

Upstream 100 bases:

>100_bases
GACGCCTTGTTTAACCTAAGACTAGGCAAGTAAAAACAACAGAATATCGCCACAAACTAAGAACAGATGTGGCAACAAAT
TGAAGATAAAGGATAATACA

Downstream 100 bases:

>100_bases
TCGTTATCAGGCAGCCTTTGCCGCCCTTAATGAGAAAAACCAAGGCGCATTTGTGCCGTTCGTGACCTTAGGCGACCCAT
CACCTGAGCTATCGCTCAAG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSKLNPYFGEYGGMYVPQILMPALKQLETAFIEAQEDEAFQEEFTDLLKNYAGRPTALTLTRNLSPNPLAKIYLKREDLL
HGGAHKTNQVLGQALLAKRMGKKEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVTS
GSATLKDACNEAMRDWSGCYDKAHYLLGTAAGPHPYPTIVREFQRMIGAETKRQILEREGRLPDAVIACVGGGSNAIGMF
ADFIDEPEVALIGVEPAGKGIDTPMHGAPLHHGKTGIFFGMKAPLMQDSEGQVEESYSVSAGLDFPSVGPQHAHLAAIGR
ATYESATDDEALETFQLLARCEGIIPALESAHAIAYAVKLAKEATKETLLVVNLSGRGDKDIFTVADILEQQKVEQQKVE
QQKADNQNTEKNNQESGNE

Sequences:

>Translated_419_residues
MSKLNPYFGEYGGMYVPQILMPALKQLETAFIEAQEDEAFQEEFTDLLKNYAGRPTALTLTRNLSPNPLAKIYLKREDLL
HGGAHKTNQVLGQALLAKRMGKKEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVTS
GSATLKDACNEAMRDWSGCYDKAHYLLGTAAGPHPYPTIVREFQRMIGAETKRQILEREGRLPDAVIACVGGGSNAIGMF
ADFIDEPEVALIGVEPAGKGIDTPMHGAPLHHGKTGIFFGMKAPLMQDSEGQVEESYSVSAGLDFPSVGPQHAHLAAIGR
ATYESATDDEALETFQLLARCEGIIPALESAHAIAYAVKLAKEATKETLLVVNLSGRGDKDIFTVADILEQQKVEQQKVE
QQKADNQNTEKNNQESGNE
>Mature_418_residues
SKLNPYFGEYGGMYVPQILMPALKQLETAFIEAQEDEAFQEEFTDLLKNYAGRPTALTLTRNLSPNPLAKIYLKREDLLH
GGAHKTNQVLGQALLAKRMGKKEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVTSG
SATLKDACNEAMRDWSGCYDKAHYLLGTAAGPHPYPTIVREFQRMIGAETKRQILEREGRLPDAVIACVGGGSNAIGMFA
DFIDEPEVALIGVEPAGKGIDTPMHGAPLHHGKTGIFFGMKAPLMQDSEGQVEESYSVSAGLDFPSVGPQHAHLAAIGRA
TYESATDDEALETFQLLARCEGIIPALESAHAIAYAVKLAKEATKETLLVVNLSGRGDKDIFTVADILEQQKVEQQKVEQ
QKADNQNTEKNNQESGNE

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=388, Percent_Identity=79.1237113402062, Blast_Score=639, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=54.1666666666667, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_SHEHH (B0TP63)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673879.1
- ProteinModelPortal:   B0TP63
- SMR:   B0TP63
- GeneID:   5905306
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1654
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   SHAL458817:SHAL_1654-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 45414; Mature: 45283

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNPYFGEYGGMYVPQILMPALKQLETAFIEAQEDEAFQEEFTDLLKNYAGRPTALTL
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEE
TRNLSPNPLAKIYLKREDLLHGGAHKTNQVLGQALLAKRMGKKEIIAETGAGQHGVATAL
ECCCCCCCHHHEEECHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHH
ACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVTSGSATLKDACNEAMRDWSGCY
HHHHHHHHHEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHH
DKAHYLLGTAAGPHPYPTIVREFQRMIGAETKRQILEREGRLPDAVIACVGGGSNAIGMF
HHHHHHEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
ADFIDEPEVALIGVEPAGKGIDTPMHGAPLHHGKTGIFFGMKAPLMQDSEGQVEESYSVS
HHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCC
AGLDFPSVGPQHAHLAAIGRATYESATDDEALETFQLLARCEGIIPALESAHAIAYAVKL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEATKETLLVVNLSGRGDKDIFTVADILEQQKVEQQKVEQQKADNQNTEKNNQESGNE
HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKLNPYFGEYGGMYVPQILMPALKQLETAFIEAQEDEAFQEEFTDLLKNYAGRPTALTL
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEE
TRNLSPNPLAKIYLKREDLLHGGAHKTNQVLGQALLAKRMGKKEIIAETGAGQHGVATAL
ECCCCCCCHHHEEECHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHH
ACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVTSGSATLKDACNEAMRDWSGCY
HHHHHHHHHEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHH
DKAHYLLGTAAGPHPYPTIVREFQRMIGAETKRQILEREGRLPDAVIACVGGGSNAIGMF
HHHHHHEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
ADFIDEPEVALIGVEPAGKGIDTPMHGAPLHHGKTGIFFGMKAPLMQDSEGQVEESYSVS
HHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCC
AGLDFPSVGPQHAHLAAIGRATYESATDDEALETFQLLARCEGIIPALESAHAIAYAVKL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEATKETLLVVNLSGRGDKDIFTVADILEQQKVEQQKVEQQKADNQNTEKNNQESGNE
HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA