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The map label for this gene is trpA [H]

Identifier: 167623584

GI number: 167623584

Start: 1983021

End: 1983830

Strand: Reverse

Name: trpA [H]

Synonym: Shal_1653

Alternate gene names: 167623584

Gene position: 1983830-1983021 (Counterclockwise)

Preceding gene: 167623585

Following gene: 167623574

Centisome position: 37.95

GC content: 52.47

Gene sequence:

>810_bases
ATGAGTAATCGTTATCAGGCAGCCTTTGCCGCCCTTAATGAGAAAAACCAAGGCGCATTTGTGCCGTTCGTGACCTTAGG
CGACCCATCACCTGAGCTATCGCTCAAGATCATCGATACCTTAGTGGAAAACGGCGCCGATGCCCTAGAGCTAGGCTTTC
CATTCTCAGATCCACTAGCCGATGGTCCGGTTATTCAAGGTGCAAACCTGCGCGCATTAGCCGCAGGCACCACGCCATCT
CAGTGCTTTGAGATGCTAGGCACTATTCGCGCTAAGTACCCAGAGCTACCTATCGGCCTGCTTTTATATGCAAACCTCGT
GTTTGCCAACGGCATCGATAAGTTTTACGGCAAATGCCAAGCCGCTGGCGTCGATTCGGTATTAATTGCCGACGTACCAG
TCGAGGAAGCCGCGCCTTTTATCGCATCAGCCAAGGCCCACGGCGTGTGCCCAATCTTTATCGCACCGCCTAATGCCGAT
AGCGAGACCTTAAAGCAGGTCAGCGAAAAAGGGGAAGGCTACACTTACCTGTTATCACGCGCGGGTGTTACTGGCACAGA
ATCGAAAGCGGGTATGCCAATTGGCGACATTCTCAATCGCCTAAAGGAGTTTAATGGTGCGCCGCCACTACTCGGTTTTG
GTATTGCCGAACCTTCGCAGGTCAAAGCGGCCATCGATGCGGGCGCAGCTGGCGCGATTTCAGGTTCTGCGGTGGTAAAA
ATCATCGCAGCCAATAAAGACAACGAACAAGCCTTATTAAGCGCTCTCGGTGAATTTACCCGCAATATGAAGGCGGCAAC
GTCTCGCTAA

Upstream 100 bases:

>100_bases
GTTGCGGACATTCTTGAGCAACAAAAAGTTGAGCAACAAAAAGTTGAGCAACAAAAAGCTGACAATCAGAATACAGAGAA
AAACAATCAGGAGAGTGGCA

Downstream 100 bases:

>100_bases
GTAGTCGAACGACAGATAAAAAAATAGCGCCTAGGGCGCTATTTTTTTGGTTGATCCGTCATCGAATATTCTTAATCAAT
GACTTATTAACTACTTAAGC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSNRYQAAFAALNEKNQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLADGPVIQGANLRALAAGTTPS
QCFEMLGTIRAKYPELPIGLLLYANLVFANGIDKFYGKCQAAGVDSVLIADVPVEEAAPFIASAKAHGVCPIFIAPPNAD
SETLKQVSEKGEGYTYLLSRAGVTGTESKAGMPIGDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVK
IIAANKDNEQALLSALGEFTRNMKAATSR

Sequences:

>Translated_269_residues
MSNRYQAAFAALNEKNQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLADGPVIQGANLRALAAGTTPS
QCFEMLGTIRAKYPELPIGLLLYANLVFANGIDKFYGKCQAAGVDSVLIADVPVEEAAPFIASAKAHGVCPIFIAPPNAD
SETLKQVSEKGEGYTYLLSRAGVTGTESKAGMPIGDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVK
IIAANKDNEQALLSALGEFTRNMKAATSR
>Mature_268_residues
SNRYQAAFAALNEKNQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLADGPVIQGANLRALAAGTTPSQ
CFEMLGTIRAKYPELPIGLLLYANLVFANGIDKFYGKCQAAGVDSVLIADVPVEEAAPFIASAKAHGVCPIFIAPPNADS
ETLKQVSEKGEGYTYLLSRAGVTGTESKAGMPIGDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVKI
IAANKDNEQALLSALGEFTRNMKAATSR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=265, Percent_Identity=66.0377358490566, Blast_Score=342, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6321412, Length=242, Percent_Identity=31.404958677686, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 27913; Mature: 27782

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRYQAAFAALNEKNQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLA
CCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPVIQGANLRALAAGTTPSQCFEMLGTIRAKYPELPIGLLLYANLVFANGIDKFYGKCQ
CCCEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
AAGVDSVLIADVPVEEAAPFIASAKAHGVCPIFIAPPNADSETLKQVSEKGEGYTYLLSR
CCCCCEEEEECCCHHHHCCHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
AGVTGTESKAGMPIGDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVK
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCEEEE
IIAANKDNEQALLSALGEFTRNMKAATSR
EEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNRYQAAFAALNEKNQGAFVPFVTLGDPSPELSLKIIDTLVENGADALELGFPFSDPLA
CCHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPVIQGANLRALAAGTTPSQCFEMLGTIRAKYPELPIGLLLYANLVFANGIDKFYGKCQ
CCCEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
AAGVDSVLIADVPVEEAAPFIASAKAHGVCPIFIAPPNADSETLKQVSEKGEGYTYLLSR
CCCCCEEEEECCCHHHHCCHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
AGVTGTESKAGMPIGDILNRLKEFNGAPPLLGFGIAEPSQVKAAIDAGAAGAISGSAVVK
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCEEEE
IIAANKDNEQALLSALGEFTRNMKAATSR
EEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]