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The map label for this gene is trpCF [H]

Identifier: 167623586

GI number: 167623586

Start: 1985187

End: 1986626

Strand: Reverse

Name: trpCF [H]

Synonym: Shal_1655

Alternate gene names: 167623586

Gene position: 1986626-1985187 (Counterclockwise)

Preceding gene: 167623587

Following gene: 167623585

Centisome position: 38.01

GC content: 48.82

Gene sequence:

>1440_bases
GTGAGCACGGATCTACGAGAAACAACGGTGGCCAAAGAAAGCAACGTATTAACTAAAATTGTTGATTCCAAAGCGGCTCA
TATCGCGTCACTGAAACAACGCTTTCCTGAGGCAAGCCTTACACCTAAGCTGTCTGATAGAAGTTTGTTTGATGCGCTTA
ACGCCCCAAATGCTGGGTTTATTCTAGAGTGCAAAAAAGCCAGCCCATCGAAAGGCTTAATCCGTGATGTATTTGATGTC
GATGCGATTAGCGACGTCTATAACCGCTACGCTGCGGGTATTTCGGTATTAACCGATGAACAGTTCTTCCAAGGTGATAT
GGACTATATTCCACGAGTGCGTGCTCGCGTCACTCAGCCTATCTTGTGCAAAGACTTCTTCGTCGACGAGTATCAGGTCA
AACTTGCCGCTCATCAGGGCGCCGATGCGATTCTATTGATGCTATCGGTACTGGATGATGCAAGATATCAAGTACTTGCA
ACCGAGGCGGCTAAGTATCAGCTGGATATCTTGACCGAAGTGAGTAACGAGGCAGAGCTGAGCCGCGCCATTGAGCTTAA
TGCTGCCATTATCGGCATTAATAACCGCAATCTTCGCGATCTATCAACCGATCTTGCTACCACAGAAGCGCTGGCGCCGC
ATATCCCAGCTGACAGAGTGGTGATCAGCGAATCGGGCATTTATAACCAAGCCCAAGTTCGCCGCTTGAGCCCACTGGTT
GATGGCTTCTTGGTGGGTAGCTCATTGATGGCCGAGGAGGATCTGGACCTTGCTTGCCGCACCCTGACTTTTGGCCACAA
CAAAGTATGCGGCCTGACCCGTATCGAAGACATGCTAGCTGTCGCCAAAGCTGGCGCCGTCTATGGTGGACTCATCTTTG
CGAAAAAGTCTCCACGAGCGGTGACAGCCGAGCAGACACAAGAGCTAGTCAAACAGCTAAAACAGAGTGGTACTAGGCTC
AATCTGGTTGGAGTATTTGTCAATGAAGCGGTCGATGTTATCGCAAAGCTCGCCGTCGATTTAGATCTATTTGCAGTGCA
ATTGCACGGTAAAGAAACCCACCTTGAAGTTAGCCAGCTAAAGGCGATTTTTGCTGAGCTAAACTGTCAAACTCAAATAT
GGAAAGCCGTTGCGGTGAGTATCGATGCTGAGGGTAATAGCGGCGATGAACTTGTTATCCCCGAAGGCATTGATCGAATC
GTATTTGATAGCAAGTCAGACAACCAATTTGGCGGCACTGGCCAAACCTTTGATTGGCAAAAGGTACTGCCAAACAGAAG
CGCAGCCTTGCTCGCGGGCGGCTTAAGCCCAGCCAATGCCACGCAAGCCGCTGCCCAAGGATTTTACGGCTTAGACTTTA
ACTCCGGCCTTGAGCAAATCCCTGGTATTAAAGATTTAGCCAAAATCACTGAGGCATTTAAGTTATTACGCCAATATTGA

Upstream 100 bases:

>100_bases
TCAGGCACGGCCTTAGCATTAGAAACAATAAAAAGCGGTAAAGCCTACGAATTATTGACCCAGTTTGCTAACGCTAGCCA
AGCTGGCAAAGGAGCCTAAT

Downstream 100 bases:

>100_bases
TTATGACGCCTTGTTTAACCTAAGACTAGGCAAGTAAAAACAACAGAATATCGCCACAAACTAAGAACAGATGTGGCAAC
AAATTGAAGATAAAGGATAA

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSTDLRETTVAKESNVLTKIVDSKAAHIASLKQRFPEASLTPKLSDRSLFDALNAPNAGFILECKKASPSKGLIRDVFDV
DAISDVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVTQPILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDARYQVLA
TEAAKYQLDILTEVSNEAELSRAIELNAAIIGINNRNLRDLSTDLATTEALAPHIPADRVVISESGIYNQAQVRRLSPLV
DGFLVGSSLMAEEDLDLACRTLTFGHNKVCGLTRIEDMLAVAKAGAVYGGLIFAKKSPRAVTAEQTQELVKQLKQSGTRL
NLVGVFVNEAVDVIAKLAVDLDLFAVQLHGKETHLEVSQLKAIFAELNCQTQIWKAVAVSIDAEGNSGDELVIPEGIDRI
VFDSKSDNQFGGTGQTFDWQKVLPNRSAALLAGGLSPANATQAAAQGFYGLDFNSGLEQIPGIKDLAKITEAFKLLRQY

Sequences:

>Translated_479_residues
MSTDLRETTVAKESNVLTKIVDSKAAHIASLKQRFPEASLTPKLSDRSLFDALNAPNAGFILECKKASPSKGLIRDVFDV
DAISDVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVTQPILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDARYQVLA
TEAAKYQLDILTEVSNEAELSRAIELNAAIIGINNRNLRDLSTDLATTEALAPHIPADRVVISESGIYNQAQVRRLSPLV
DGFLVGSSLMAEEDLDLACRTLTFGHNKVCGLTRIEDMLAVAKAGAVYGGLIFAKKSPRAVTAEQTQELVKQLKQSGTRL
NLVGVFVNEAVDVIAKLAVDLDLFAVQLHGKETHLEVSQLKAIFAELNCQTQIWKAVAVSIDAEGNSGDELVIPEGIDRI
VFDSKSDNQFGGTGQTFDWQKVLPNRSAALLAGGLSPANATQAAAQGFYGLDFNSGLEQIPGIKDLAKITEAFKLLRQY
>Mature_478_residues
STDLRETTVAKESNVLTKIVDSKAAHIASLKQRFPEASLTPKLSDRSLFDALNAPNAGFILECKKASPSKGLIRDVFDVD
AISDVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVTQPILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDARYQVLAT
EAAKYQLDILTEVSNEAELSRAIELNAAIIGINNRNLRDLSTDLATTEALAPHIPADRVVISESGIYNQAQVRRLSPLVD
GFLVGSSLMAEEDLDLACRTLTFGHNKVCGLTRIEDMLAVAKAGAVYGGLIFAKKSPRAVTAEQTQELVKQLKQSGTRLN
LVGVFVNEAVDVIAKLAVDLDLFAVQLHGKETHLEVSQLKAIFAELNCQTQIWKAVAVSIDAEGNSGDELVIPEGIDRIV
FDSKSDNQFGGTGQTFDWQKVLPNRSAALLAGGLSPANATQAAAQGFYGLDFNSGLEQIPGIKDLAKITEAFKLLRQY

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain [H]

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=471, Percent_Identity=49.0445859872611, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6322638, Length=232, Percent_Identity=32.7586206896552, Blast_Score=103, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00218 IGPS; PF00697 PRAI [H]

EC number: =4.1.1.48; =5.3.1.24 [H]

Molecular weight: Translated: 52077; Mature: 51946

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00614 IGPS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDLRETTVAKESNVLTKIVDSKAAHIASLKQRFPEASLTPKLSDRSLFDALNAPNAGF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCE
ILECKKASPSKGLIRDVFDVDAISDVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVTQP
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCHHHHHHHHHHCCCC
ILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDARYQVLATEAAKYQLDILTEVSNEAEL
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHEEEEHHHHCCCHHHH
SRAIELNAAIIGINNRNLRDLSTDLATTEALAPHIPADRVVISESGIYNQAQVRRLSPLV
HHHEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHH
DGFLVGSSLMAEEDLDLACRTLTFGHNKVCGLTRIEDMLAVAKAGAVYGGLIFAKKSPRA
HHHHHCCHHHCCCCCCCEEHEEECCCCCCCCHHHHHHHHHHHHHCHHHEEEEEECCCCCC
VTAEQTQELVKQLKQSGTRLNLVGVFVNEAVDVIAKLAVDLDLFAVQLHGKETHLEVSQL
CCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHH
KAIFAELNCQTQIWKAVAVSIDAEGNSGDELVIPEGIDRIVFDSKSDNQFGGTGQTFDWQ
HHHHHHHCCHHHEEEEEEEEEECCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCHH
KVLPNRSAALLAGGLSPANATQAAAQGFYGLDFNSGLEQIPGIKDLAKITEAFKLLRQY
HHCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STDLRETTVAKESNVLTKIVDSKAAHIASLKQRFPEASLTPKLSDRSLFDALNAPNAGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCE
ILECKKASPSKGLIRDVFDVDAISDVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVTQP
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCHHHHHHHHHHCCCC
ILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDARYQVLATEAAKYQLDILTEVSNEAEL
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHEEEEHHHHCCCHHHH
SRAIELNAAIIGINNRNLRDLSTDLATTEALAPHIPADRVVISESGIYNQAQVRRLSPLV
HHHEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHH
DGFLVGSSLMAEEDLDLACRTLTFGHNKVCGLTRIEDMLAVAKAGAVYGGLIFAKKSPRA
HHHHHCCHHHCCCCCCCEEHEEECCCCCCCCHHHHHHHHHHHHHCHHHEEEEEECCCCCC
VTAEQTQELVKQLKQSGTRLNLVGVFVNEAVDVIAKLAVDLDLFAVQLHGKETHLEVSQL
CCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHH
KAIFAELNCQTQIWKAVAVSIDAEGNSGDELVIPEGIDRIVFDSKSDNQFGGTGQTFDWQ
HHHHHHHCCHHHEEEEEEEEEECCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCHH
KVLPNRSAALLAGGLSPANATQAAAQGFYGLDFNSGLEQIPGIKDLAKITEAFKLLRQY
HHCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]