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The map label for this gene is truD [H]

Identifier: 167623160

GI number: 167623160

Start: 1490131

End: 1491195

Strand: Direct

Name: truD [H]

Synonym: Shal_1226

Alternate gene names: 167623160

Gene position: 1490131-1491195 (Clockwise)

Preceding gene: 167623159

Following gene: 167623161

Centisome position: 28.51

GC content: 45.26

Gene sequence:

>1065_bases
ATGAGTGAATTACATTATTTATACGGCAAGCCAACTTCAACTGCCGATCTACGTACCCACAATAGTGATTTCCAAGTGCA
GGAGATTTTACCTTTCGCGCCGACAGGTGAGGGGGAGCATCATCTGCTACACATACGTAAAGATGGGCTCAATACCCTTG
AAATCGTTAAGAGGATCTCGATCTTTGCTCATGTACACCCAAAAGAGGTGACTTATGCCGGGCAAAAAGATAAGCATGCG
GTTACAGAGCAGTGGTTTGGTGTACGTATTCCAGGTAAAGAAACGCCTGACTGGCAGCAGCTCAATAGCGACCAAGTTAC
AGTGCTTAACGCTGCAAGACATAGTAAAAAACTGCGGATTGGCGCGCTGGCTGGTAATCGCTTTACCTTAATATTGCGTA
ATGTCAGCGATATGAGTGATGTGATTAGACGCATTGACTTAGTCAAAGGGCAGGGCGTACCAAACTATTTTGGTGAGCAG
CGATTTGGGCATGATGGTAAAAATTTAGAGTTTGGTCGGCAGATGTTTACTGGCCGTAAAGTTAAAGATCGTAATAAGCG
CAGTATGTATTTGTCGGCAGTTCGCTCGAATGTGTTTAATATGGCCTGTTCAGGTCGTTTAGCGCAGCATGGTACTAAAG
CCTTAGCAGGTGATTGTGTCATGCTTGCAGGCAGTAAAAGTTACTTTGTTGCCGAGCAGTGGGATGAAACGTTAATAGAG
CGTCTAAATAATAAAGATATTCAGCTTTCAGCACCATTGTGGGGGCGTGGCTTACCACTTGCTCAGTCTGAAGCGGCGCA
GTGTGAGGCTGACGCACTTGAGTCACTCGCAATTGACAAAGACGGTTTAGAGCATGCAGGTCTTAGCCAAGAGAGACGTG
CTTTGTTACTTGAGCCGCAACATCTGCAATATCAAGTAGATGAAGATACCATTACCCTTAAATTTGCATTACCATCTGGG
TCTTATGCAACCTCAGTACTACGTGAGTTATTCCAGTATCAAGATGTTCAAGAGGTCGCGCGTCAACAGATGCTAGCTGA
TCAAAGAAGCGCTGAGGCCGATTAA

Upstream 100 bases:

>100_bases
AAAAGCCTAGCCAATCGTGTGGGCAAGAAGCTATAGCTAGCAATAGTGGCAATAGATTTTTATGAGATAGCAAGATACTT
TAGCGAGACAATTTAGCGAT

Downstream 100 bases:

>100_bases
TGAAGATTTTAATCAGTAATGATGATGGGGTGAACGCAGAAGGTATTGCTGCCTTAACGACCGCATTAAACCAGATAGCA
GAAACGCTCACCGTAGGGCC

Product: tRNA pseudouridine synthase D TruD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKDGLNTLEIVKRISIFAHVHPKEVTYAGQKDKHA
VTEQWFGVRIPGKETPDWQQLNSDQVTVLNAARHSKKLRIGALAGNRFTLILRNVSDMSDVIRRIDLVKGQGVPNYFGEQ
RFGHDGKNLEFGRQMFTGRKVKDRNKRSMYLSAVRSNVFNMACSGRLAQHGTKALAGDCVMLAGSKSYFVAEQWDETLIE
RLNNKDIQLSAPLWGRGLPLAQSEAAQCEADALESLAIDKDGLEHAGLSQERRALLLEPQHLQYQVDEDTITLKFALPSG
SYATSVLRELFQYQDVQEVARQQMLADQRSAEAD

Sequences:

>Translated_354_residues
MSELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKDGLNTLEIVKRISIFAHVHPKEVTYAGQKDKHA
VTEQWFGVRIPGKETPDWQQLNSDQVTVLNAARHSKKLRIGALAGNRFTLILRNVSDMSDVIRRIDLVKGQGVPNYFGEQ
RFGHDGKNLEFGRQMFTGRKVKDRNKRSMYLSAVRSNVFNMACSGRLAQHGTKALAGDCVMLAGSKSYFVAEQWDETLIE
RLNNKDIQLSAPLWGRGLPLAQSEAAQCEADALESLAIDKDGLEHAGLSQERRALLLEPQHLQYQVDEDTITLKFALPSG
SYATSVLRELFQYQDVQEVARQQMLADQRSAEAD
>Mature_353_residues
SELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKDGLNTLEIVKRISIFAHVHPKEVTYAGQKDKHAV
TEQWFGVRIPGKETPDWQQLNSDQVTVLNAARHSKKLRIGALAGNRFTLILRNVSDMSDVIRRIDLVKGQGVPNYFGEQR
FGHDGKNLEFGRQMFTGRKVKDRNKRSMYLSAVRSNVFNMACSGRLAQHGTKALAGDCVMLAGSKSYFVAEQWDETLIER
LNNKDIQLSAPLWGRGLPLAQSEAAQCEADALESLAIDKDGLEHAGLSQERRALLLEPQHLQYQVDEDTITLKFALPSGS
YATSVLRELFQYQDVQEVARQQMLADQRSAEAD

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI148762985, Length=148, Percent_Identity=32.4324324324324, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI148762961, Length=148, Percent_Identity=32.4324324324324, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI148762948, Length=148, Percent_Identity=32.4324324324324, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789102, Length=335, Percent_Identity=45.6716417910448, Blast_Score=262, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 39839; Mature: 39707

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKDGLNTLEIVKRIS
CCCCEEEECCCCCCCCCEECCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
IFAHVHPKEVTYAGQKDKHAVTEQWFGVRIPGKETPDWQQLNSDQVTVLNAARHSKKLRI
HHHCCCCHHEEECCCCCCHHHHHHHCEEECCCCCCCCHHHCCCCCEEEEECHHCCCEEEE
GALAGNRFTLILRNVSDMSDVIRRIDLVKGQGVPNYFGEQRFGHDGKNLEFGRQMFTGRK
EEEECCEEEEEEECHHHHHHHHHHHHHHCCCCCCCHHCCHHCCCCCCCCHHHHHHHCCCC
VKDRNKRSMYLSAVRSNVFNMACSGRLAQHGTKALAGDCVMLAGSKSYFVAEQWDETLIE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHCCCEEEEECCCCEEEHHHHHHHHHH
RLNNKDIQLSAPLWGRGLPLAQSEAAQCEADALESLAIDKDGLEHAGLSQERRALLLEPQ
HHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEECCC
HLQYQVDEDTITLKFALPSGSYATSVLRELFQYQDVQEVARQQMLADQRSAEAD
CCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SELHYLYGKPTSTADLRTHNSDFQVQEILPFAPTGEGEHHLLHIRKDGLNTLEIVKRIS
CCCEEEECCCCCCCCCEECCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
IFAHVHPKEVTYAGQKDKHAVTEQWFGVRIPGKETPDWQQLNSDQVTVLNAARHSKKLRI
HHHCCCCHHEEECCCCCCHHHHHHHCEEECCCCCCCCHHHCCCCCEEEEECHHCCCEEEE
GALAGNRFTLILRNVSDMSDVIRRIDLVKGQGVPNYFGEQRFGHDGKNLEFGRQMFTGRK
EEEECCEEEEEEECHHHHHHHHHHHHHHCCCCCCCHHCCHHCCCCCCCCHHHHHHHCCCC
VKDRNKRSMYLSAVRSNVFNMACSGRLAQHGTKALAGDCVMLAGSKSYFVAEQWDETLIE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHCCCEEEEECCCCEEEHHHHHHHHHH
RLNNKDIQLSAPLWGRGLPLAQSEAAQCEADALESLAIDKDGLEHAGLSQERRALLLEPQ
HHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEEECCC
HLQYQVDEDTITLKFALPSGSYATSVLRELFQYQDVQEVARQQMLADQRSAEAD
CCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]