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The map label for this gene is rlmA [H]

Identifier: 167622953

GI number: 167622953

Start: 1244069

End: 1244902

Strand: Direct

Name: rlmA [H]

Synonym: Shal_1014

Alternate gene names: 167622953

Gene position: 1244069-1244902 (Clockwise)

Preceding gene: 167622952

Following gene: 167622954

Centisome position: 23.8

GC content: 44.6

Gene sequence:

>834_bases
ATGAATAGTATTTATCTTTGTCCTGTATGTCAGTCACCTTTTATGGTGCATGAAGAGTCGAAAGGCTTGCACTGTGAGAA
CAAGCATCACTTTGACATTAATGAGCAGGGCTACTGGGTGTTTAGCCAACCCAAAAAACCTAAAGCAGACTCACGCGCAG
TGATGCGTGCTAAACGCTATGTACTTGAGTCGGGGATCTATATCCCGCTGGCACAAGCGATTGCCAAGGTGATTGCTGAA
TTACCTCAAGTCACCTCTGCTGAGGCGTTATCTCAGCTTGACTTTGATTGTGGTGATGGTTACTTCTTGCGTACGGTTAA
GGGGATTTTAGCTGAATTAAAGCCTGAACTGGCATTAACGCAAACGGGTATTTGCGAAGCCGAGAATGCAATATTTGCCG
CGGCTAAAGCAGAGCCTAGCCCAACCTATATCGTCTCGACATTGAAGCACTTACCTTTTGCTAATGACAGTTTTGACTTG
GTGACCTTAATTGACAAGCCACTCAAAGGCAAAGAGTTAACGCGCATTTTGAAGCAAGACGGCGTGTTGCTGCAAGTGGC
TGCAGGTCCAAGGCATTTATGGCAAATACGTGAGTTTATCTACCCTGACTTATCTGAAAAAGCGGTCAGTGATAATTTAC
CGAAAGAGCTCGAATTACAGCATAGTGAGCGCTTATCTCTGACTGCAAGCGTGTCAGGCGAACAGGCTATTGCGCTACTT
GAGATGACGCCTTATGCATGGCGTGCAAATGATAAGGTGCGTCATCAAATTCAACATGCTCAGTTCGATGCACTAGAAAT
CGACTTTGTTATTAATGTGATGACTAGAAAATAA

Upstream 100 bases:

>100_bases
TCGCCTCGCATTAGGTGCGGTGTTATGTGGTTTTATCTTTTTATAATTGTAGGGTAAGCCACCCTATAATTTATCATTCA
AGCTAATTGATGAGCGTGTT

Downstream 100 bases:

>100_bases
TCTGCGCTCAGGGTAATCTTTGCTTATTCAACTAAACGGAAACCGAAAAACTGATGATGCGTAATTTGCTGTTGTTCATC
TTGGCATTTTTGCCACTGGC

Product: rRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNSIYLCPVCQSPFMVHEESKGLHCENKHHFDINEQGYWVFSQPKKPKADSRAVMRAKRYVLESGIYIPLAQAIAKVIAE
LPQVTSAEALSQLDFDCGDGYFLRTVKGILAELKPELALTQTGICEAENAIFAAAKAEPSPTYIVSTLKHLPFANDSFDL
VTLIDKPLKGKELTRILKQDGVLLQVAAGPRHLWQIREFIYPDLSEKAVSDNLPKELELQHSERLSLTASVSGEQAIALL
EMTPYAWRANDKVRHQIQHAQFDALEIDFVINVMTRK

Sequences:

>Translated_277_residues
MNSIYLCPVCQSPFMVHEESKGLHCENKHHFDINEQGYWVFSQPKKPKADSRAVMRAKRYVLESGIYIPLAQAIAKVIAE
LPQVTSAEALSQLDFDCGDGYFLRTVKGILAELKPELALTQTGICEAENAIFAAAKAEPSPTYIVSTLKHLPFANDSFDL
VTLIDKPLKGKELTRILKQDGVLLQVAAGPRHLWQIREFIYPDLSEKAVSDNLPKELELQHSERLSLTASVSGEQAIALL
EMTPYAWRANDKVRHQIQHAQFDALEIDFVINVMTRK
>Mature_277_residues
MNSIYLCPVCQSPFMVHEESKGLHCENKHHFDINEQGYWVFSQPKKPKADSRAVMRAKRYVLESGIYIPLAQAIAKVIAE
LPQVTSAEALSQLDFDCGDGYFLRTVKGILAELKPELALTQTGICEAENAIFAAAKAEPSPTYIVSTLKHLPFANDSFDL
VTLIDKPLKGKELTRILKQDGVLLQVAAGPRHLWQIREFIYPDLSEKAVSDNLPKELELQHSERLSLTASVSGEQAIALL
EMTPYAWRANDKVRHQIQHAQFDALEIDFVINVMTRK

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=276, Percent_Identity=31.8840579710145, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 31115; Mature: 31115

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIYLCPVCQSPFMVHEESKGLHCENKHHFDINEQGYWVFSQPKKPKADSRAVMRAKRY
CCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
VLESGIYIPLAQAIAKVIAELPQVTSAEALSQLDFDCGDGYFLRTVKGILAELKPELALT
HHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHEC
QTGICEAENAIFAAAKAEPSPTYIVSTLKHLPFANDSFDLVTLIDKPLKGKELTRILKQD
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHC
GVLLQVAAGPRHLWQIREFIYPDLSEKAVSDNLPKELELQHSERLSLTASVSGEQAIALL
CEEEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCEEEEE
EMTPYAWRANDKVRHQIQHAQFDALEIDFVINVMTRK
EECCCEECCCHHHHHHHHHHHCCEEEEEEEEEEEECC
>Mature Secondary Structure
MNSIYLCPVCQSPFMVHEESKGLHCENKHHFDINEQGYWVFSQPKKPKADSRAVMRAKRY
CCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
VLESGIYIPLAQAIAKVIAELPQVTSAEALSQLDFDCGDGYFLRTVKGILAELKPELALT
HHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHEC
QTGICEAENAIFAAAKAEPSPTYIVSTLKHLPFANDSFDLVTLIDKPLKGKELTRILKQD
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHC
GVLLQVAAGPRHLWQIREFIYPDLSEKAVSDNLPKELELQHSERLSLTASVSGEQAIALL
CEEEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCEEEEE
EMTPYAWRANDKVRHQIQHAQFDALEIDFVINVMTRK
EECCCEECCCHHHHHHHHHHHCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]