Click here to switch to the map view.

The map label for this gene is dhfrIII [H]

Identifier: 167622850

GI number: 167622850

Start: 1129261

End: 1129743

Strand: Direct

Name: dhfrIII [H]

Synonym: Shal_0910

Alternate gene names: 167622850

Gene position: 1129261-1129743 (Clockwise)

Preceding gene: 167622849

Following gene: 167622851

Centisome position: 21.6

GC content: 44.1

Gene sequence:

>483_bases
ATGAAAATAGCTATGATTGCTGCGATGGCAAATAACCGCGTCATCGGTAAAGATAACCAAATGCCTTGGCACCTTCCTGA
AGACTTACGCCATTTTAAAGCTATGACCTTAGGTAAACCTGTAGTGATGGGACGAAAGACGTATGAGTCGATAGGACGCC
CATTACCGGGACGTCATAATATCGTCATTAGTCGTCAGACCGATCTATCTATCGATGGTGTGACAATCGTCAGCAGTTTT
GCGGAGGCTAAGATTGCCGCCGGGGAGTGTGAGGAACTAGTGGTGATGGGCGGAGGCCAGCTATATGAGATGTTGCTACC
TCAAGCGGATATTTTGTACCTTACTGAAATCGCATTAACGGTAGATGGTGATACCTACTTCCCTGAGTGGGATGATGGTA
GCTGGCAAGAAGTTTCTAGAAATGTAGCTGAAGATGACAAACAACTTGAATATAGCTTTATCAAGTTGGTTAAAAAATGT
TAA

Upstream 100 bases:

>100_bases
AGTTCATTCATTTAGAACTGTTAATCAAGGGTTTGTTGATTAAAGGTTTGATGAAATCTATATATCCACTGTAGCCGACA
TAAAAAAAAAGGGAAGTATC

Downstream 100 bases:

>100_bases
ATTCAAAGGGTTGTTTAGTACCCTGTATAACAAAAAAAGAGCGCTACTAGATAGCGCTACTTTTCTAATAAATAAAAACT
AAAAAATAAGGAGTGTCTGA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHNIVISRQTDLSIDGVTIVSSF
AEAKIAAGECEELVVMGGGQLYEMLLPQADILYLTEIALTVDGDTYFPEWDDGSWQEVSRNVAEDDKQLEYSFIKLVKKC

Sequences:

>Translated_160_residues
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHNIVISRQTDLSIDGVTIVSSF
AEAKIAAGECEELVVMGGGQLYEMLLPQADILYLTEIALTVDGDTYFPEWDDGSWQEVSRNVAEDDKQLEYSFIKLVKKC
>Mature_160_residues
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHNIVISRQTDLSIDGVTIVSSF
AEAKIAAGECEELVVMGGGQLYEMLLPQADILYLTEIALTVDGDTYFPEWDDGSWQEVSRNVAEDDKQLEYSFIKLVKKC

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=146, Percent_Identity=34.2465753424658, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1786233, Length=151, Percent_Identity=50.9933774834437, Blast_Score=154, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17505899, Length=180, Percent_Identity=30, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116008026, Length=157, Percent_Identity=35.6687898089172, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24647458, Length=157, Percent_Identity=35.6687898089172, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17932; Mature: 17932

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHN
CCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVISRQTDLSIDGVTIVSSFAEAKIAAGECEELVVMGGGQLYEMLLPQADILYLTEIALT
EEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCEEEEEEEEEE
VDGDTYFPEWDDGSWQEVSRNVAEDDKQLEYSFIKLVKKC
ECCCEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIAMIAAMANNRVIGKDNQMPWHLPEDLRHFKAMTLGKPVVMGRKTYESIGRPLPGRHN
CCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVISRQTDLSIDGVTIVSSFAEAKIAAGECEELVVMGGGQLYEMLLPQADILYLTEIALT
EEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCEEEEEEEEEE
VDGDTYFPEWDDGSWQEVSRNVAEDDKQLEYSFIKLVKKC
ECCCEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]