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The map label for this gene is phoB [H]

Identifier: 167622639

GI number: 167622639

Start: 861775

End: 863085

Strand: Reverse

Name: phoB [H]

Synonym: Shal_0699

Alternate gene names: 167622639

Gene position: 863085-861775 (Counterclockwise)

Preceding gene: 167622640

Following gene: 167622638

Centisome position: 16.51

GC content: 50.04

Gene sequence:

>1311_bases
ATGAACTTAACAAAAACACTCACTTGGACCATTAGCGCCCTAGTTTTGTTGCCCCTATTTGCAGCGGCTAACGTCGATAT
TCACGATGCGCCATCACGCCCTAAGAATATGATTATTATGATAGGTGATGGCATGGGCCCGGCGTACACCAGTGCCTATC
GTTACTACCAAGATAATCCTGATACCGAAGAGATAGAGCAAACTGTTTTTGATAGGTTACTTGTCGGCATGGCATCGACT
TATCCAGCAAGGAAAAGTGGTTATGTGACTGATTCTGCCGCCTCAGCGACGGCGCTTGCCACTGGCGTTAAGAGCTATAA
CGGTGCCATCTCGGTTGACGTAGACAAGCGCCCGCTAACCACCATTATGCAGATGGCTAAAGCGCGCGGCATGGCAACTG
GCGTGGCCGTCACCTCTCAAGTCAATCATGCTACCCCCGCCGCGTTTCTTACTCATAATGAAAGCCGTCGTAATTACGAC
GAAATAGCGCAAAGCTACTTAAGCTCTGATACCGACGTCATACTCGGTGGGGGACAGAAGTTCTTCCCTGAAGCGTTACT
TGAACAATTTACCGCCAAGGGTTACCAGCATATTAGCCAGCTTAGCCAGCTCGATACGCTAACCCAAGGTAAAGCCTTAG
GTCTGTTTGCCGAAGTGCAGATGCCATGGGTTATCGATCAACAAGACTCGGCTCAGTTAAGCTTGCTGACCCAAAAGGCC
TTAGAGCTACTGTCACAAAACGAAAAAGGCTTTGTACTACTGGTTGAAGGTAGCCTAATTGACTGGGCGGGGCATAACAA
CGATATCGCCAGCGCCATGGGCGAGATGCATGGCTTTGCCAATGCCATCGAAGTTGTCGAGCAATTTGTGCGCGCTAACC
CAGACACCTTGATGGTTATTACTGCCGATCATAATACCGGCGGCCTCTCTATCGGAGCCAATGGCGAATATGCTTGGGAT
ACCGCTTTACTCCAAGGTATTCAAGCAAGCCCTGCTACCATAGCAGAGCAAGCGATCGCCATCGATGATTGGCAAGCGTT
TGTGAGTCAACAGCTAGGCTTTGAACTCAAGCAAGATGAATTGCAGCGCCTGAGTAATGCGCGTATGCAAGGTAAGGTCG
CACTCGAAACCAGCCTAAAACAGCTTATCGATAGTCGTAGCTTCACCGGCTGGACCACCAGTGGTCACACGGGTGGCGAT
GTACAGGTTTTTGCCGCGGGCCCTGCGGCCGATCTGTTTAAAGGCAATCAAGACAATACCAATATCGCCGAAAAAATGAT
GAGTTTATTGCCACGTCAGGAGCAGCCGTAG

Upstream 100 bases:

>100_bases
TCAAAGAAATTGAAGCGGCGTTTGATGTCGACATCACCGGCATGTTGATGGATGCTATCGAAGCACGCATTAATAAGTCG
GCTTAGCCAAAGGATCCGGC

Downstream 100 bases:

>100_bases
TCAATACGCTTATCATTTAATACGCTTCACTTAAATCAGGAAAGGCCGCTGTCATCTGCGGTCTTTTTTATACCTATCCA
ATCTTCGTCAACACGCAAAA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNLTKTLTWTISALVLLPLFAAANVDIHDAPSRPKNMIIMIGDGMGPAYTSAYRYYQDNPDTEEIEQTVFDRLLVGMAST
YPARKSGYVTDSAASATALATGVKSYNGAISVDVDKRPLTTIMQMAKARGMATGVAVTSQVNHATPAAFLTHNESRRNYD
EIAQSYLSSDTDVILGGGQKFFPEALLEQFTAKGYQHISQLSQLDTLTQGKALGLFAEVQMPWVIDQQDSAQLSLLTQKA
LELLSQNEKGFVLLVEGSLIDWAGHNNDIASAMGEMHGFANAIEVVEQFVRANPDTLMVITADHNTGGLSIGANGEYAWD
TALLQGIQASPATIAEQAIAIDDWQAFVSQQLGFELKQDELQRLSNARMQGKVALETSLKQLIDSRSFTGWTTSGHTGGD
VQVFAAGPAADLFKGNQDNTNIAEKMMSLLPRQEQP

Sequences:

>Translated_436_residues
MNLTKTLTWTISALVLLPLFAAANVDIHDAPSRPKNMIIMIGDGMGPAYTSAYRYYQDNPDTEEIEQTVFDRLLVGMAST
YPARKSGYVTDSAASATALATGVKSYNGAISVDVDKRPLTTIMQMAKARGMATGVAVTSQVNHATPAAFLTHNESRRNYD
EIAQSYLSSDTDVILGGGQKFFPEALLEQFTAKGYQHISQLSQLDTLTQGKALGLFAEVQMPWVIDQQDSAQLSLLTQKA
LELLSQNEKGFVLLVEGSLIDWAGHNNDIASAMGEMHGFANAIEVVEQFVRANPDTLMVITADHNTGGLSIGANGEYAWD
TALLQGIQASPATIAEQAIAIDDWQAFVSQQLGFELKQDELQRLSNARMQGKVALETSLKQLIDSRSFTGWTTSGHTGGD
VQVFAAGPAADLFKGNQDNTNIAEKMMSLLPRQEQP
>Mature_436_residues
MNLTKTLTWTISALVLLPLFAAANVDIHDAPSRPKNMIIMIGDGMGPAYTSAYRYYQDNPDTEEIEQTVFDRLLVGMAST
YPARKSGYVTDSAASATALATGVKSYNGAISVDVDKRPLTTIMQMAKARGMATGVAVTSQVNHATPAAFLTHNESRRNYD
EIAQSYLSSDTDVILGGGQKFFPEALLEQFTAKGYQHISQLSQLDTLTQGKALGLFAEVQMPWVIDQQDSAQLSLLTQKA
LELLSQNEKGFVLLVEGSLIDWAGHNNDIASAMGEMHGFANAIEVVEQFVRANPDTLMVITADHNTGGLSIGANGEYAWD
TALLQGIQASPATIAEQAIAIDDWQAFVSQQLGFELKQDELQRLSNARMQGKVALETSLKQLIDSRSFTGWTTSGHTGGD
VQVFAAGPAADLFKGNQDNTNIAEKMMSLLPRQEQP

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=457, Percent_Identity=28.2275711159737, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI94721246, Length=462, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI157266292, Length=450, Percent_Identity=28, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI116734717, Length=459, Percent_Identity=26.797385620915, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI294660770, Length=455, Percent_Identity=26.3736263736264, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI48994877, Length=344, Percent_Identity=36.6279069767442, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320689, Length=482, Percent_Identity=29.6680497925311, Blast_Score=173, Evalue=5e-44,
Organism=Drosophila melanogaster, GI21355981, Length=449, Percent_Identity=26.5033407572383, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21355151, Length=456, Percent_Identity=26.5350877192982, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI85725200, Length=444, Percent_Identity=26.8018018018018, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI85815833, Length=444, Percent_Identity=26.8018018018018, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21358067, Length=460, Percent_Identity=25.6521739130435, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24657842, Length=456, Percent_Identity=25.6578947368421, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18859923, Length=413, Percent_Identity=25.90799031477, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24651554, Length=459, Percent_Identity=27.4509803921569, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24657835, Length=455, Percent_Identity=25.7142857142857, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24657827, Length=456, Percent_Identity=25.6578947368421, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 47204; Mature: 47204

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTKTLTWTISALVLLPLFAAANVDIHDAPSRPKNMIIMIGDGMGPAYTSAYRYYQDNP
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
DTEEIEQTVFDRLLVGMASTYPARKSGYVTDSAASATALATGVKSYNGAISVDVDKRPLT
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHH
TIMQMAKARGMATGVAVTSQVNHATPAAFLTHNESRRNYDEIAQSYLSSDTDVILGGGQK
HHHHHHHHCCCHHHHHHCCCCCCCCCHHEEECCCCCCCHHHHHHHHHCCCCCEEEECCCH
FFPEALLEQFTAKGYQHISQLSQLDTLTQGKALGLFAEVQMPWVIDQQDSAQLSLLTQKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCHHHHHHHHHH
LELLSQNEKGFVLLVEGSLIDWAGHNNDIASAMGEMHGFANAIEVVEQFVRANPDTLMVI
HHHHCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TADHNTGGLSIGANGEYAWDTALLQGIQASPATIAEQAIAIDDWQAFVSQQLGFELKQDE
EECCCCCCEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LQRLSNARMQGKVALETSLKQLIDSRSFTGWTTSGHTGGDVQVFAAGPAADLFKGNQDNT
HHHHHCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCH
NIAEKMMSLLPRQEQP
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNLTKTLTWTISALVLLPLFAAANVDIHDAPSRPKNMIIMIGDGMGPAYTSAYRYYQDNP
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
DTEEIEQTVFDRLLVGMASTYPARKSGYVTDSAASATALATGVKSYNGAISVDVDKRPLT
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHH
TIMQMAKARGMATGVAVTSQVNHATPAAFLTHNESRRNYDEIAQSYLSSDTDVILGGGQK
HHHHHHHHCCCHHHHHHCCCCCCCCCHHEEECCCCCCCHHHHHHHHHCCCCCEEEECCCH
FFPEALLEQFTAKGYQHISQLSQLDTLTQGKALGLFAEVQMPWVIDQQDSAQLSLLTQKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCHHHHHHHHHH
LELLSQNEKGFVLLVEGSLIDWAGHNNDIASAMGEMHGFANAIEVVEQFVRANPDTLMVI
HHHHCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TADHNTGGLSIGANGEYAWDTALLQGIQASPATIAEQAIAIDDWQAFVSQQLGFELKQDE
EECCCCCCEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LQRLSNARMQGKVALETSLKQLIDSRSFTGWTTSGHTGGDVQVFAAGPAADLFKGNQDNT
HHHHHCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCH
NIAEKMMSLLPRQEQP
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]