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The map label for this gene is tapB [H]

Identifier: 167622410

GI number: 167622410

Start: 556756

End: 558462

Strand: Reverse

Name: tapB [H]

Synonym: Shal_0470

Alternate gene names: 167622410

Gene position: 558462-556756 (Counterclockwise)

Preceding gene: 167622411

Following gene: 167622409

Centisome position: 10.68

GC content: 49.91

Gene sequence:

>1707_bases
ATGCCTACAACAGGTCTCCACCTAGGACTATCTACTCTCTTTATTCGCAAAGGATTACTTAGCGAAGAGCAGATCGCATC
GGCAATATCCACCTCACGTAAACATAAGACATCGCTCGTCAGCACTATCATCAGCAATAAACTGATCTCAGCACGGGATA
TCGCCGAGCTCTGCTATGAAGAGTACGGCACACCGCTACTCGACCTCGATGAATTCGATATTGGTTCCATTCCTGAAGAG
TTTCTCAATAAGAAGCTGATCGAGAAGCACAAATGCCTGCCGCTATTTAAACGCGGTAACCGCCTCTATATCGCTACATC
TGATCCCACCAATATTGCAGCGTTGGAAGACTTTCAATTCAGTGCTGGCCTGCACGCCGAAGCCATTCTGGTTGAAGACG
ATAAGCTGATTAAGGCACTTGAAACTATTCTTGAAGAGGATATCAGTGCGCTGGATCTCGATGGTATAGACGAAGAATCC
CTTGCAGGTATAGAGGTCACCGATACCGATAAGCGTGATGAAGAGGAAAATAGTGGCAAAGATGATGCGCCGATTGTCAT
CTATATCAATAAAATTCTCACCGATGCCATCCGTAAGGGCGCATCAGACTTGCATTTCGAGCCCTACGAGAAACGCTACC
GCATCCGCTTTCGCATCGACGGTATCTTGCATGAAGTCTCCGAACCGCCAGTTAATCTTTCCGGCCGCATCTCGGCGCGC
TTAAAGGTGATGTCAAAACTTGATATCGCCGAGCGCCGCGTACCGCAAGATGGTCGTATCAAGATGAAGCTGTCGCGCAC
CAAGTCCATCGACTTTCGTGTCAGCACCCTGCCCACTATCTGGGGCGAGAAGATAGTAATGCGTATCTTGGACTCGTCTT
CGGCGCAATTAGGTATCGAGAAGCTCGGCTATGAGGAAGACCAAAGATTATTGTACGAAGAGATGCTGCAAAAACCTCAG
GGGATGATCCTCGTCACCGGCCCTACCGGCTCGGGTAAGACGGTATCGCTTTATACTGGCCTCAATATTCTCAATACCGA
AGAGCGCAATATCTCTACCGCCGAAGATCCGGTCGAGATCAACCTCGAAGGGGTCAATCAGGTTCATATTAATTTAAAGG
CGGGCTTAACCTTCGCCTCGGCGCTGCGCTCATTCCTACGTCAAGATCCCGATGTGGTGATGGTGGGTGAAATCCGAGAC
TTGGAGACCGCAGAGATTGCCATCAAGGCGGCGCAAACCGGTCACTTGGTATTGTCGACACTGCACACCAACTCTGCAGC
CGAGACTCTGACGCGATTAATCAACATGGGCGTTCCCGGCTATAATATCGCCAGCTCGGTCAACCTGATTATTGCCCAGC
GATTATCCAGGCGCCTCTGCCCTAATTGTAAGGCACCGGAAGATATCCCCGCAGCAGAACTACTAAAACTAGGGTTTAAC
CAACAGCAAGTCGATGCCGGGATCACCCCCCACAAACCTGTAGGCTGCGATCAATGCTCCGGCGGCTATAAAGGCCGAGT
CGGTATCTATGAGGTGATGAAGATGTCAGATGAGATAGCCCGAACCATCATGGAGGGCGGCAATTCACTGCAAATTCTTA
AGCAAGCCAAACATCAGGGGATGCGCGACCTGCGGGATTCTGGACTGTTAAAGGTGATTCAAGGTGTTACCAGTATCGCT
GAAATAAACCGTGTCACCAGTTTTTAG

Upstream 100 bases:

>100_bases
ATAAAGGCTGGTGTTCAGAATTCTAAGAAAGCTTAGATGAAAGGATAACCACAATAAATTTTGGTTATCCTTTCATTACT
TTTAAGCGATACAATATTTT

Downstream 100 bases:

>100_bases
TCCCTCCTATTTTAACGATAACACCATATATCAAAAGGATACCTTATGGCGACGGCAACAATGAAAACTAAAAAGCCCAG
TGCCAAGAAAACAAAAAACC

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MPTTGLHLGLSTLFIRKGLLSEEQIASAISTSRKHKTSLVSTIISNKLISARDIAELCYEEYGTPLLDLDEFDIGSIPEE
FLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLIKALETILEEDISALDLDGIDEES
LAGIEVTDTDKRDEEENSGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEEDQRLLYEEMLQKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPNCKAPEDIPAAELLKLGFN
QQQVDAGITPHKPVGCDQCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKHQGMRDLRDSGLLKVIQGVTSIA
EINRVTSF

Sequences:

>Translated_568_residues
MPTTGLHLGLSTLFIRKGLLSEEQIASAISTSRKHKTSLVSTIISNKLISARDIAELCYEEYGTPLLDLDEFDIGSIPEE
FLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLIKALETILEEDISALDLDGIDEES
LAGIEVTDTDKRDEEENSGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEEDQRLLYEEMLQKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPNCKAPEDIPAAELLKLGFN
QQQVDAGITPHKPVGCDQCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKHQGMRDLRDSGLLKVIQGVTSIA
EINRVTSF
>Mature_567_residues
PTTGLHLGLSTLFIRKGLLSEEQIASAISTSRKHKTSLVSTIISNKLISARDIAELCYEEYGTPLLDLDEFDIGSIPEEF
LNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLIKALETILEEDISALDLDGIDEESL
AGIEVTDTDKRDEEENSGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISARL
KVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEEDQRLLYEEMLQKPQG
MILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPNCKAPEDIPAAELLKLGFNQ
QQVDAGITPHKPVGCDQCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKHQGMRDLRDSGLLKVIQGVTSIAE
INRVTSF

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=386, Percent_Identity=49.4818652849741, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1786296, Length=429, Percent_Identity=43.1235431235431, Blast_Score=342, Evalue=5e-95,
Organism=Escherichia coli, GI87082188, Length=281, Percent_Identity=33.8078291814947, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62830; Mature: 62699

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTGLHLGLSTLFIRKGLLSEEQIASAISTSRKHKTSLVSTIISNKLISARDIAELCYE
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EYGTPLLDLDEFDIGSIPEEFLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQF
HHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCEEEEECCCC
SAGLHAEAILVEDDKLIKALETILEEDISALDLDGIDEESLAGIEVTDTDKRDEEENSGK
CCCCCEEEEEECCHHHHHHHHHHHHHCCCEECCCCCCHHHCCCEEEECCCCCCCHHCCCC
DDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
CCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCHHHH
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIE
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHH
KLGYEEDQRLLYEEMLQKPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEI
HCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEECCCCCCCCCCCCCEEE
NLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVLST
EEECCEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCCEEEEE
LHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPNCKAPEDIPAAELLKLGFN
ECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
QQQVDAGITPHKPVGCDQCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKHQG
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MRDLRDSGLLKVIQGVTSIAEINRVTSF
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTTGLHLGLSTLFIRKGLLSEEQIASAISTSRKHKTSLVSTIISNKLISARDIAELCYE
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EYGTPLLDLDEFDIGSIPEEFLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQF
HHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCEEEEECCCC
SAGLHAEAILVEDDKLIKALETILEEDISALDLDGIDEESLAGIEVTDTDKRDEEENSGK
CCCCCEEEEEECCHHHHHHHHHHHHHCCCEECCCCCCHHHCCCEEEECCCCCCCHHCCCC
DDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
CCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCHHHH
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIE
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHH
KLGYEEDQRLLYEEMLQKPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEI
HCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEECCCCCCCCCCCCCEEE
NLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVLST
EEECCEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCCEEEEE
LHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPNCKAPEDIPAAELLKLGFN
ECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCC
QQQVDAGITPHKPVGCDQCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKHQG
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MRDLRDSGLLKVIQGVTSIAEINRVTSF
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]