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The map label for this gene is trmH [H]

Identifier: 167622328

GI number: 167622328

Start: 455259

End: 455945

Strand: Reverse

Name: trmH [H]

Synonym: Shal_0388

Alternate gene names: 167622328

Gene position: 455945-455259 (Counterclockwise)

Preceding gene: 167622329

Following gene: 167622327

Centisome position: 8.72

GC content: 51.09

Gene sequence:

>687_bases
ATGAGTCCTGAACGTTTCGCCCGTATCAACCAGATGCTTGATAACCGCCAACCCGATCTCACCGTATGTCTAGATACCGT
GCATAAAGGCAATAACATTGCCGCCGTGCTGCGTACCGCTGATGCGGCGGGCATACATGAAGTCCACGCGGTTTGGTCTG
AACTAGAAATGCGCGTATCGGGCAATACGGCCTCAGGTAGCCAACAATGGGTAAAGACGCGCGTGCACCAGAGCATGCAA
CAAGCCGTCGATGAGTTTCGTGGCAAAGAGATGCAAATTTTGGCTACCACCTTTTCTGATACCGCAGTCGATTTTAGAGA
GATTGATTACACTCGACCGACCGCGATTATTTTGGGTAACGAGCGCGACGGTGTCAGCCCTGCAGGCATCGCGGCAGCCG
ATCAACATATCATTATCCCGATGATAGGCATGGTGCAATCACTTAACGTCTCGGTTGCCGCGGCGCTGATTATGTATGAA
GCCCAACGCCAACGTACAGCTGCGGGCATGTACGGTACACGTAAGCTTGATGATGCCTATTGTCAAATAAAGCTGTTTGA
GGAAGGCCACCCAATTTATGCCAAGGCCTGCCGTCGTAAGAAACTACCTTACCCAGCCATAGATGAAGCAGGCCAAATCG
TTGCCGATGAAGATTGGTGGCAAAAAATGCGTGCGCCTTTAGACTAA

Upstream 100 bases:

>100_bases
AATGGATGGAGTCATGGAGATCTAATCTTCCACTCCTCTACCAAATGGGTGCTACGGCGCCCTTTTATTGTTAAAACGTC
TTACTAATTAGAAGTCCATC

Downstream 100 bases:

>100_bases
GCGTAAAGCCCATCAAAAAATACCAGCAGCCCTGCTGGTATTTTTTTATCTCTAACAACGAAATCACCCAGCATATTAAA
ATTACCGCCTACGCCCTCTC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVSGNTASGSQQWVKTRVHQSMQ
QAVDEFRGKEMQILATTFSDTAVDFREIDYTRPTAIILGNERDGVSPAGIAAADQHIIIPMIGMVQSLNVSVAAALIMYE
AQRQRTAAGMYGTRKLDDAYCQIKLFEEGHPIYAKACRRKKLPYPAIDEAGQIVADEDWWQKMRAPLD

Sequences:

>Translated_228_residues
MSPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVSGNTASGSQQWVKTRVHQSMQ
QAVDEFRGKEMQILATTFSDTAVDFREIDYTRPTAIILGNERDGVSPAGIAAADQHIIIPMIGMVQSLNVSVAAALIMYE
AQRQRTAAGMYGTRKLDDAYCQIKLFEEGHPIYAKACRRKKLPYPAIDEAGQIVADEDWWQKMRAPLD
>Mature_227_residues
SPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVSGNTASGSQQWVKTRVHQSMQQ
AVDEFRGKEMQILATTFSDTAVDFREIDYTRPTAIILGNERDGVSPAGIAAADQHIIIPMIGMVQSLNVSVAAALIMYEA
QRQRTAAGMYGTRKLDDAYCQIKLFEEGHPIYAKACRRKKLPYPAIDEAGQIVADEDWWQKMRAPLD

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=227, Percent_Identity=57.2687224669604, Blast_Score=273, Evalue=7e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25458; Mature: 25327

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVS
CCHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEC
GNTASGSQQWVKTRVHQSMQQAVDEFRGKEMQILATTFSDTAVDFREIDYTRPTAIILGN
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCEEEEEEC
ERDGVSPAGIAAADQHIIIPMIGMVQSLNVSVAAALIMYEAQRQRTAAGMYGTRKLDDAY
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
CQIKLFEEGHPIYAKACRRKKLPYPAIDEAGQIVADEDWWQKMRAPLD
EEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCC
>Mature Secondary Structure 
SPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVS
CHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEC
GNTASGSQQWVKTRVHQSMQQAVDEFRGKEMQILATTFSDTAVDFREIDYTRPTAIILGN
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCEEEEEEC
ERDGVSPAGIAAADQHIIIPMIGMVQSLNVSVAAALIMYEAQRQRTAAGMYGTRKLDDAY
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
CQIKLFEEGHPIYAKACRRKKLPYPAIDEAGQIVADEDWWQKMRAPLD
EEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]