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The map label for this gene is fadD [C]

Identifier: 167622327

GI number: 167622327

Start: 453340

End: 454995

Strand: Reverse

Name: fadD [C]

Synonym: Shal_0387

Alternate gene names: 167622327

Gene position: 454995-453340 (Counterclockwise)

Preceding gene: 167622328

Following gene: 167622326

Centisome position: 8.7

GC content: 47.83

Gene sequence:

>1656_bases
ATGGAATCTTCTATGAAAACCCCAATTGAGATGCTTGAACATTGGGTTGAAAAACAAGGTGATCAAGTTTACCTAAAGCA
ACCTATTGACGGCCAGTATAAAACCTTTACTTGGCGCGATGTGCAAACCAAGATGCAGCAGATTGCAGGCGCACTGAGAC
ATCTAGGCCTTAACCCTGGTGATAAGATTGCCGTGCTGTCTAAAAACTGTGCAGAGTGGTTTATTACCGATCTCGCCCTG
ATGCACGGTGGTTATATTAGTGTGCCTATCTACCCTACCGCCAATGCTGACACCATTCGCTACACCTTAGAACACAGTGA
ATCTAAGGCGATTTTTATCGGTAAGCTAGATTATTGGGCCGACCAAGAAGCAGGCGTCGGCGGTGATATCCTGCGTATCG
CCATGCCTTACGACACGATGCCGTCTCAGTACCACTGGGACAAAATGCTAACCTTAGGTCAACCACTAATCGATGCCCCT
CTACCGACTGCCGAGCAGGTGATGACCATCATCTACACTTCAGGCTCAACCGGTAGGCCTAAAGGGGCTATTCAAAATTT
TGCCAGCTATGTCTGGACCTGCACCGCCGTAGTGCGCGACCTTAAAACTGGTGTTGAAGATAGATTGCTTTCTTATCTTC
CACTGGCACACATTACCGAGCGTGTGGCGATTGAAGGTTCATCCTTCTACTCAGGTAGCAGCGTCGCCTTTGTAGAGAGC
CTAGATAGCTTCGTTGCCGATGTACAGCGCGCAAGACCAACGGTGTTCTTCTCGGTGCCACGCCTGTGGAGCCTATTCCA
AAAGAACATTATCGACAAGATTGGCTACAGCAAACTCAACTTTTTGCTTAAAGTGCCGATCATTAGTGGCATAGTAAAAC
GTAAAATCCATCAAGGTTTAGGCTTAGAGCATTGCCACCTGCTTGGTTCTGGCTCTGCACCAATCCCACCATCGCTCGTT
GAGTGGTACCACAAGATAGGGCTCGATATCAGCGAAGCCTGGGGCATGACAGAAAACTGTGCCTACTCAATTATCAACTA
TCCGTTTGATCCCCGTAAAATTGGCTCGGTTGGTCGCGCGATAGAAGGCTGTGAAGCCAAGTGTTCGGATGTTGGTGAAC
TGCTAGTTAAGAGCCCAGGCTTGATGCAAGGCTATTACAAGCAGGAAGAAGAGAGCGCTAAGTGCTTCGATAAAGAGGGT
TTCTTTCACACTGGTGACTTATGCACTATCGATGATGAAGGCAATGTCAGCATTACTGGCCGTGTTAAAGACAACTTCAA
AACATCGAAAGGTAAGTATGTGGCTCCAGTGCCTATTGAGCGCAAACTAGCACAAGATCCTCACATCGAGATGCTCTGTG
TTATCGGCTCAGGTTTACCGCACCCTATCGCCTTAGTTCAGCTCTCTGAAGGTGCCGCGGTTCAACCAAGAGAAGAGGTA
AGGCTTTCTCTCAAAAACACCATAGATTCGGTCAATCCACACCTTGAATCCCATGAGACTGTCGATGCTATTGTTATCGT
CACTGAAGCTTGGGATGTCGAGAATGACGTGTTAACACCGACCCTGAAAATTAAACGCCATGTACTGGAGAAGAAGTTTA
GCGACAAGGTCGATGGCATCCGTGGTGGCAAGGTCTGTTGGGAAGATGAAATCTAG

Upstream 100 bases:

>100_bases
CGCCATAATCATTAATTATACAGCTTATTAAACGAACGATTAAAACAAGAATAGATAACTAGGCTGAACTGAAATTAACT
ACTATCAATATGGAAATGCG

Downstream 100 bases:

>100_bases
CAATTACTAGCGATTTCCCCCATAAACGGTACACTTTTCAGTGTGCCGTTTTTTATTTAGAGCAAAAATCACCCAATTAA
CTAAATTTAATGGTGGATGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase; LACS [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPGDKIAVLSKNCAEWFITDLAL
MHGGYISVPIYPTANADTIRYTLEHSESKAIFIGKLDYWADQEAGVGGDILRIAMPYDTMPSQYHWDKMLTLGQPLIDAP
LPTAEQVMTIIYTSGSTGRPKGAIQNFASYVWTCTAVVRDLKTGVEDRLLSYLPLAHITERVAIEGSSFYSGSSVAFVES
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISGIVKRKIHQGLGLEHCHLLGSGSAPIPPSLV
EWYHKIGLDISEAWGMTENCAYSIINYPFDPRKIGSVGRAIEGCEAKCSDVGELLVKSPGLMQGYYKQEEESAKCFDKEG
FFHTGDLCTIDDEGNVSITGRVKDNFKTSKGKYVAPVPIERKLAQDPHIEMLCVIGSGLPHPIALVQLSEGAAVQPREEV
RLSLKNTIDSVNPHLESHETVDAIVIVTEAWDVENDVLTPTLKIKRHVLEKKFSDKVDGIRGGKVCWEDEI

Sequences:

>Translated_551_residues
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPGDKIAVLSKNCAEWFITDLAL
MHGGYISVPIYPTANADTIRYTLEHSESKAIFIGKLDYWADQEAGVGGDILRIAMPYDTMPSQYHWDKMLTLGQPLIDAP
LPTAEQVMTIIYTSGSTGRPKGAIQNFASYVWTCTAVVRDLKTGVEDRLLSYLPLAHITERVAIEGSSFYSGSSVAFVES
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISGIVKRKIHQGLGLEHCHLLGSGSAPIPPSLV
EWYHKIGLDISEAWGMTENCAYSIINYPFDPRKIGSVGRAIEGCEAKCSDVGELLVKSPGLMQGYYKQEEESAKCFDKEG
FFHTGDLCTIDDEGNVSITGRVKDNFKTSKGKYVAPVPIERKLAQDPHIEMLCVIGSGLPHPIALVQLSEGAAVQPREEV
RLSLKNTIDSVNPHLESHETVDAIVIVTEAWDVENDVLTPTLKIKRHVLEKKFSDKVDGIRGGKVCWEDEI
>Mature_551_residues
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPGDKIAVLSKNCAEWFITDLAL
MHGGYISVPIYPTANADTIRYTLEHSESKAIFIGKLDYWADQEAGVGGDILRIAMPYDTMPSQYHWDKMLTLGQPLIDAP
LPTAEQVMTIIYTSGSTGRPKGAIQNFASYVWTCTAVVRDLKTGVEDRLLSYLPLAHITERVAIEGSSFYSGSSVAFVES
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISGIVKRKIHQGLGLEHCHLLGSGSAPIPPSLV
EWYHKIGLDISEAWGMTENCAYSIINYPFDPRKIGSVGRAIEGCEAKCSDVGELLVKSPGLMQGYYKQEEESAKCFDKEG
FFHTGDLCTIDDEGNVSITGRVKDNFKTSKGKYVAPVPIERKLAQDPHIEMLCVIGSGLPHPIALVQLSEGAAVQPREEV
RLSLKNTIDSVNPHLESHETVDAIVIVTEAWDVENDVLTPTLKIKRHVLEKKFSDKVDGIRGGKVCWEDEI

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI83745141, Length=617, Percent_Identity=23.9870340356564, Blast_Score=185, Evalue=9e-47,
Organism=Homo sapiens, GI42794756, Length=611, Percent_Identity=25.0409165302782, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI42794760, Length=611, Percent_Identity=25.0409165302782, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI42794758, Length=611, Percent_Identity=25.0409165302782, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI40807491, Length=581, Percent_Identity=26.1617900172117, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI57165412, Length=583, Percent_Identity=25.2144082332762, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI27477105, Length=496, Percent_Identity=23.7903225806452, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI57165410, Length=583, Percent_Identity=24.8713550600343, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI4758332, Length=622, Percent_Identity=24.1157556270096, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI12669909, Length=622, Percent_Identity=24.1157556270096, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI42794754, Length=626, Percent_Identity=23.1629392971246, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI42794752, Length=626, Percent_Identity=23.1629392971246, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI156151445, Length=474, Percent_Identity=22.3628691983122, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI253970489, Length=356, Percent_Identity=23.5955056179775, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI8923896, Length=356, Percent_Identity=23.5955056179775, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1788107, Length=488, Percent_Identity=22.7459016393443, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693145, Length=459, Percent_Identity=22.4400871459695, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=24.6435845213849, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786810, Length=447, Percent_Identity=24.1610738255034, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789201, Length=287, Percent_Identity=27.1777003484321, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17556552, Length=590, Percent_Identity=26.7796610169492, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17510401, Length=577, Percent_Identity=27.209705372617, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI25147511, Length=613, Percent_Identity=24.1435562805873, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17541856, Length=567, Percent_Identity=26.8077601410935, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17553312, Length=628, Percent_Identity=25.3184713375796, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI193204819, Length=588, Percent_Identity=25.1700680272109, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17564090, Length=533, Percent_Identity=25.891181988743, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17558820, Length=297, Percent_Identity=29.96632996633, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=540, Percent_Identity=22.4074074074074, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=499, Percent_Identity=23.4468937875752, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71994703, Length=499, Percent_Identity=23.4468937875752, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=499, Percent_Identity=23.4468937875752, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=323, Percent_Identity=28.1733746130031, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=458, Percent_Identity=26.6375545851528, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI133901848, Length=218, Percent_Identity=29.3577981651376, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=138, Percent_Identity=36.9565217391304, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=331, Percent_Identity=24.1691842900302, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320852, Length=577, Percent_Identity=25.8232235701906, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324893, Length=595, Percent_Identity=24.7058823529412, Blast_Score=140, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6323903, Length=592, Percent_Identity=25.5067567567568, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322182, Length=585, Percent_Identity=23.5897435897436, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319699, Length=546, Percent_Identity=21.7948717948718, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17933690, Length=617, Percent_Identity=24.4732576985413, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI281366413, Length=577, Percent_Identity=24.6100519930676, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24666501, Length=580, Percent_Identity=24.6551724137931, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24666497, Length=580, Percent_Identity=24.6551724137931, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19921316, Length=496, Percent_Identity=24.3951612903226, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI62471689, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI62471679, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI62471683, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI62471685, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24586636, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI62471681, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI62471687, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24586634, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI22026970, Length=600, Percent_Identity=23.1666666666667, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21358303, Length=450, Percent_Identity=23.7777777777778, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI18859661, Length=549, Percent_Identity=24.9544626593807, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21355181, Length=493, Percent_Identity=23.9350912778905, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356441, Length=530, Percent_Identity=22.8301886792453, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365686, Length=134, Percent_Identity=34.3283582089552, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61333; Mature: 61333

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPG
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCC
DKIAVLSKNCAEWFITDLALMHGGYISVPIYPTANADTIRYTLEHSESKAIFIGKLDYWA
CEEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCC
DQEAGVGGDILRIAMPYDTMPSQYHWDKMLTLGQPLIDAPLPTAEQVMTIIYTSGSTGRP
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHCCCCCCHHHEEEEEEECCCCCCC
KGAIQNFASYVWTCTAVVRDLKTGVEDRLLSYLPLAHITERVAIEGSSFYSGSSVAFVES
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHH
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISGIVKRKIHQGL
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCHHHHHHHEECHHHHHHHHHHHHHCC
GLEHCHLLGSGSAPIPPSLVEWYHKIGLDISEAWGMTENCAYSIINYPFDPRKIGSVGRA
CCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHH
IEGCEAKCSDVGELLVKSPGLMQGYYKQEEESAKCFDKEGFFHTGDLCTIDDEGNVSITG
HHCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCEECCCEEEECCCCCEEEEE
RVKDNFKTSKGKYVAPVPIERKLAQDPHIEMLCVIGSGLPHPIALVQLSEGAAVQPREEV
EECCCCCCCCCCEECCCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCHHHH
RLSLKNTIDSVNPHLESHETVDAIVIVTEAWDVENDVLTPTLKIKRHVLEKKFSDKVDGI
HHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
RGGKVCWEDEI
CCCCEEECCCC
>Mature Secondary Structure
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPG
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCC
DKIAVLSKNCAEWFITDLALMHGGYISVPIYPTANADTIRYTLEHSESKAIFIGKLDYWA
CEEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCC
DQEAGVGGDILRIAMPYDTMPSQYHWDKMLTLGQPLIDAPLPTAEQVMTIIYTSGSTGRP
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHCCCCCCHHHEEEEEEECCCCCCC
KGAIQNFASYVWTCTAVVRDLKTGVEDRLLSYLPLAHITERVAIEGSSFYSGSSVAFVES
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHH
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISGIVKRKIHQGL
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCHHHHHHHEECHHHHHHHHHHHHHCC
GLEHCHLLGSGSAPIPPSLVEWYHKIGLDISEAWGMTENCAYSIINYPFDPRKIGSVGRA
CCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHH
IEGCEAKCSDVGELLVKSPGLMQGYYKQEEESAKCFDKEGFFHTGDLCTIDDEGNVSITG
HHCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCEECCCEEEECCCCCEEEEE
RVKDNFKTSKGKYVAPVPIERKLAQDPHIEMLCVIGSGLPHPIALVQLSEGAAVQPREEV
EECCCCCCCCCCEECCCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCHHHH
RLSLKNTIDSVNPHLESHETVDAIVIVTEAWDVENDVLTPTLKIKRHVLEKKFSDKVDGI
HHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
RGGKVCWEDEI
CCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]