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The map label for this gene is fmt

Identifier: 16752391

GI number: 16752391

Start: 108913

End: 109878

Strand: Direct

Name: fmt

Synonym: CP0098

Alternate gene names: 16752391

Gene position: 108913-109878 (Clockwise)

Preceding gene: 16752390

Following gene: 16752392

Centisome position: 8.86

GC content: 44.82

Gene sequence:

>966_bases
TTGAATCTTAAGGTTGTCTATTTCGGCACACCCACATTTGCAGCCACCGTTTTGCAAGATTTGTTGCATCACAAAATCCA
AATTACAGCTGTTGTTACCCGAGTTGATAAGCCCCAAAAAAGATCGGCACAACTCATTCCTTCTCCAGTAAAAACTATAG
CTCTAACTCACGGTCTACCTTTACTCCAACCTAGTAAAGCGTCAGACCCCCAATTTATTGAAGAACTTCGGGCTTTTAAT
GCTGATGTTTTTATTGTGGTTGCCTATGGAGCGATTCTTCGTCAGATAGTCCTCGATATTCCTCGTTATGGTTGTTACAA
TCTGCATGCAGGGCTCTTGCCAGCCTACCGAGGCGCAGCTCCCATACAACGCTGTATTATGGAAGGAGCTACAGAATCTG
GAAATACTGTCATCCGTATGGATGCAGGTATGGATACAGGCGACATGGCAAATATTACGCGTGTCCCCATAGGTCCTGAT
ATGACTTCAGGAGAATTGGCGGATGCTCTTGCATCGCAGGGCGCAGAAGTTCTAATAAAAACTTTACAACAAATTGAGTC
AGGTCAACTGCAACTCGTCAGTCAAGACGCTGCTTTGGCGACAATAGCGCCAAAATTGTCTAAGGAAGAAGGACAGGTTC
CTTGGGATAAACCTGCTAAAGAGGCTTATGCTCATATACGAGGAGTCACGCCAGCTCCAGGAGCGTGGACCCTATTCTCT
TTTTCTGAAAAAGCGCCAAAACGCTTGATGATCCGTAAGGCCTCTCTTTTAGCAGAGGCGGGAAGATATGGAGCCCCCGG
GACTGTTGTTGTAACAGATAGGCAAGAGCTTGCTGTTGCCTGTTCTGAGGGAGCCATTTGCTTGCACGAAGTGCAGGTAG
AAGGCAAGGGTTCTACGAACTCAAAATCCTTTTTAAACGGCTATCCAGCAAAGAAATTAAAAATAGTTTTTACTTTAAAT
AATTAA

Upstream 100 bases:

>100_bases
TTCATTGAGTTTTGTCAAAGCCCAAGTAAGCGCGGCATCGAACGAAGCATAGATAAGCAAGCCCTTGAAGAAGAAAGCGC
AGACAAAGAAGGAGTTTTGA

Downstream 100 bases:

>100_bases
CTTTACAACGTGTTTTTTATTTAGGGCATTTAAACTAAATTAAGTCGTTTTATGTTATTATAGGCTCTAATTTTTTTTTA
AAAAGCCTTGTAAGGAGGTA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLPLLQPSKASDPQFIEELRAFN
ADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAAPIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPD
MTSGELADALASQGAEVLIKTLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAVACSEGAICLHEVQVEGKGSTNSKSFLNGYPAKKLKIVFTLN
N

Sequences:

>Translated_321_residues
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLPLLQPSKASDPQFIEELRAFN
ADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAAPIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPD
MTSGELADALASQGAEVLIKTLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAVACSEGAICLHEVQVEGKGSTNSKSFLNGYPAKKLKIVFTLN
N
>Mature_321_residues
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLPLLQPSKASDPQFIEELRAFN
ADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAAPIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPD
MTSGELADALASQGAEVLIKTLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAVACSEGAICLHEVQVEGKGSTNSKSFLNGYPAKKLKIVFTLN
N

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=321, Percent_Identity=27.4143302180685, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI21614513, Length=241, Percent_Identity=25.7261410788382, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI238814322, Length=241, Percent_Identity=25.3112033195021, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789683, Length=306, Percent_Identity=40.8496732026144, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788589, Length=285, Percent_Identity=27.0175438596491, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI133930964, Length=252, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45550868, Length=325, Percent_Identity=27.0769230769231, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28571984, Length=279, Percent_Identity=27.2401433691756, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585660, Length=250, Percent_Identity=24.8, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34526; Mature: 34526

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLP
CCEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
LLQPSKASDPQFIEELRAFNADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAA
CCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHH
PIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPDMTSGELADALASQGAEVLIK
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCHHHHHH
TLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
HHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEE
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAVACSEGAICLHEVQVEGKGSTN
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHEEEEECCCCEEEEEEEEECCCCCC
SKSFLNGYPAKKLKIVFTLNN
CCHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MNLKVVYFGTPTFAATVLQDLLHHKIQITAVVTRVDKPQKRSAQLIPSPVKTIALTHGLP
CCEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
LLQPSKASDPQFIEELRAFNADVFIVVAYGAILRQIVLDIPRYGCYNLHAGLLPAYRGAA
CCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHH
PIQRCIMEGATESGNTVIRMDAGMDTGDMANITRVPIGPDMTSGELADALASQGAEVLIK
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCHHHHHH
TLQQIESGQLQLVSQDAALATIAPKLSKEEGQVPWDKPAKEAYAHIRGVTPAPGAWTLFS
HHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEE
FSEKAPKRLMIRKASLLAEAGRYGAPGTVVVTDRQELAVACSEGAICLHEVQVEGKGSTN
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHEEEEECCCCEEEEEEEEECCCCCC
SKSFLNGYPAKKLKIVFTLNN
CCHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]