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Identifier: 16752367

GI number: 16752367

Start: 84916

End: 86010

Strand: Direct

Name: Not Available

Synonym: CP0073

Alternate gene names: 16752367

Gene position: 84916-86010 (Clockwise)

Preceding gene: 16752366

Following gene: 16752370

Centisome position: 6.9

GC content: 41.1

Gene sequence:

>1095_bases
ATGGTTCCTTTTCGTCAGCATCATGCTTATCAGCTGTTAAAACAGCTACACACGTCTGCAATTTCAGAAGCAGATAGAGT
TTCGTACTATTTTAAACAAAACCGTTCTTTGGGGTCTAAGGATCGTCAGTGGATTCAAAATATTATTTTTAATATTTTGC
GTCATCGCCGTCTTCTTGAAACTTTAATTCTTGATTCGGGAGAACAAGTAACTCCAGAAGCTCTCGTTGCGAAAGTAAAC
GAAGGGGTTCTAGAGAACTTGGACAGTTACAGCGCTATTCCCTGGCCTGTGCGTTACTCTATATCTGATGATCTTGCGCA
CTTCTTAGTTCAAGATTATGGAGAAGAACAGGCCGAGGAGATCGCCAAGATTTGGTTAACGGAAGCTCCGATTACGATTC
GTGTGAATACAGATAAAATCTCCGTCAAAGAACTCCAAGAGAAGTTAGAATATCCAAGTTCTCCTGGAGAGCTTCCTGAG
GCATTGCACTTTTCCAAACGTCATCCCTTACAATCTACAGAAGCATTTCGTCGTGGGTTTTTCGAAATCCAAGATGAAAA
CTCTCAGAGAATTTCTCAGGGCATTTCCCTAACAGATAAAGATATCGTCTTGGATTTTTGTGCAGGAGCAGGCGGGAAAA
GCCTTATCTTTGCGCAGAAAGCAAAACATGTTGTGATCAATGACAGTCGTAAAGCTATTTTGCAAACTGCAAAGCATCGT
TTATTACGTGCTGGAGCTAGGAATTTTTCTTTAGCGGATCAATTACGTTTAGGATCCTTTTCTGTAGTTATCGTAGACGC
TCCTTGTTCTGGAACGGGAGTTTTCCGACGACATCCCGAACATAAGTGGCAATTTTCTAAGAAATTGTTGTTGAACTATG
TGCGAGTGCAAAAGAGCATCTTAAAACAAGCAAGTGCTTATGTGGGACCTCGGGGACGACTTGTCTACATTACCTGTTCT
CTTTTAAAAGAAGAAAATGAGGCTCATGTTGCCTACATGCACTCTTTAGGTTGGAAAGAAGTCCACAGAAAAACGCTTCC
CTTACAAGTAGGTAAGGGCGACGCATTTTTTACTTCTCATTTTCAAAAAATCTAG

Upstream 100 bases:

>100_bases
GTAATGCATATTTTTTACTATACTGATGGCTCTCTTTATTTTGTCTTCTGTAAGATTTTCCAAGCAAAATAGGTGATGAC
GAGGTATACTCTATGTCACT

Downstream 100 bases:

>100_bases
AATAGAGGCGCTGTAGAGCGCCCGAATCTTTCAAAAGAAATCAAACATTACCGTTCCCTAAAGTAACGATAAGAAGCTCT
ATCTTTGGTATGCAGAGTTT

Product: Nol1/Nop2/Sun family protein

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MVPFRQHHAYQLLKQLHTSAISEADRVSYYFKQNRSLGSKDRQWIQNIIFNILRHRRLLETLILDSGEQVTPEALVAKVN
EGVLENLDSYSAIPWPVRYSISDDLAHFLVQDYGEEQAEEIAKIWLTEAPITIRVNTDKISVKELQEKLEYPSSPGELPE
ALHFSKRHPLQSTEAFRRGFFEIQDENSQRISQGISLTDKDIVLDFCAGAGGKSLIFAQKAKHVVINDSRKAILQTAKHR
LLRAGARNFSLADQLRLGSFSVVIVDAPCSGTGVFRRHPEHKWQFSKKLLLNYVRVQKSILKQASAYVGPRGRLVYITCS
LLKEENEAHVAYMHSLGWKEVHRKTLPLQVGKGDAFFTSHFQKI

Sequences:

>Translated_364_residues
MVPFRQHHAYQLLKQLHTSAISEADRVSYYFKQNRSLGSKDRQWIQNIIFNILRHRRLLETLILDSGEQVTPEALVAKVN
EGVLENLDSYSAIPWPVRYSISDDLAHFLVQDYGEEQAEEIAKIWLTEAPITIRVNTDKISVKELQEKLEYPSSPGELPE
ALHFSKRHPLQSTEAFRRGFFEIQDENSQRISQGISLTDKDIVLDFCAGAGGKSLIFAQKAKHVVINDSRKAILQTAKHR
LLRAGARNFSLADQLRLGSFSVVIVDAPCSGTGVFRRHPEHKWQFSKKLLLNYVRVQKSILKQASAYVGPRGRLVYITCS
LLKEENEAHVAYMHSLGWKEVHRKTLPLQVGKGDAFFTSHFQKI
>Mature_364_residues
MVPFRQHHAYQLLKQLHTSAISEADRVSYYFKQNRSLGSKDRQWIQNIIFNILRHRRLLETLILDSGEQVTPEALVAKVN
EGVLENLDSYSAIPWPVRYSISDDLAHFLVQDYGEEQAEEIAKIWLTEAPITIRVNTDKISVKELQEKLEYPSSPGELPE
ALHFSKRHPLQSTEAFRRGFFEIQDENSQRISQGISLTDKDIVLDFCAGAGGKSLIFAQKAKHVVINDSRKAILQTAKHR
LLRAGARNFSLADQLRLGSFSVVIVDAPCSGTGVFRRHPEHKWQFSKKLLLNYVRVQKSILKQASAYVGPRGRLVYITCS
LLKEENEAHVAYMHSLGWKEVHRKTLPLQVGKGDAFFTSHFQKI

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=222, Percent_Identity=29.2792792792793, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI76150623, Length=222, Percent_Identity=29.2792792792793, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI2367212, Length=226, Percent_Identity=28.3185840707965, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87081985, Length=223, Percent_Identity=28.6995515695067, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536757, Length=224, Percent_Identity=27.6785714285714, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=235, Percent_Identity=27.2340425531915, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI22024126, Length=242, Percent_Identity=28.099173553719, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 41543; Mature: 41543

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPFRQHHAYQLLKQLHTSAISEADRVSYYFKQNRSLGSKDRQWIQNIIFNILRHRRLLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLILDSGEQVTPEALVAKVNEGVLENLDSYSAIPWPVRYSISDDLAHFLVQDYGEEQAEE
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHCHHHHHH
IAKIWLTEAPITIRVNTDKISVKELQEKLEYPSSPGELPEALHFSKRHPLQSTEAFRRGF
HHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCC
FEIQDENSQRISQGISLTDKDIVLDFCAGAGGKSLIFAQKAKHVVINDSRKAILQTAKHR
EEECCCCHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHH
LLRAGARNFSLADQLRLGSFSVVIVDAPCSGTGVFRRHPEHKWQFSKKLLLNYVRVQKSI
HHHHCCCCCCHHHHHCCCCEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
LKQASAYVGPRGRLVYITCSLLKEENEAHVAYMHSLGWKEVHRKTLPLQVGKGDAFFTSH
HHHHHHHCCCCCCEEEEEEEHHHCCCCHHHHHHHHCCHHHHHHHCCCEEECCCCHHHHHH
FQKI
HHCC
>Mature Secondary Structure
MVPFRQHHAYQLLKQLHTSAISEADRVSYYFKQNRSLGSKDRQWIQNIIFNILRHRRLLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLILDSGEQVTPEALVAKVNEGVLENLDSYSAIPWPVRYSISDDLAHFLVQDYGEEQAEE
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHCHHHHHH
IAKIWLTEAPITIRVNTDKISVKELQEKLEYPSSPGELPEALHFSKRHPLQSTEAFRRGF
HHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCC
FEIQDENSQRISQGISLTDKDIVLDFCAGAGGKSLIFAQKAKHVVINDSRKAILQTAKHR
EEECCCCHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHH
LLRAGARNFSLADQLRLGSFSVVIVDAPCSGTGVFRRHPEHKWQFSKKLLLNYVRVQKSI
HHHHCCCCCCHHHHHCCCCEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
LKQASAYVGPRGRLVYITCSLLKEENEAHVAYMHSLGWKEVHRKTLPLQVGKGDAFFTSH
HHHHHHHCCCCCCEEEEEEEHHHCCCCHHHHHHHHCCHHHHHHHCCCEEECCCCHHHHHH
FQKI
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]