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The map label for this gene is polA [H]

Identifier: 167040779

GI number: 167040779

Start: 2159100

End: 2161718

Strand: Reverse

Name: polA [H]

Synonym: Teth514_2156

Alternate gene names: 167040779

Gene position: 2161718-2159100 (Counterclockwise)

Preceding gene: 167040780

Following gene: 167040778

Centisome position: 87.97

GC content: 30.55

Gene sequence:

>2619_bases
ATGTCTAAATTTTTAGTAATTGACGGGAGTAGCCTCATGTACAGAGCCTATTATGCATTGCCTATGCTTACTACAAGTGA
AGGATTACATACAAATGCTTTGTACGGTTTTACTATGATGCTTATAAAACTTATTGAAGAGGAAAAACCTGATTACATAG
CTATTGCTTTTGACAAAAAAGCTCCTACTTTTAGACACAAAGAGTATCAAGACTACAAAGCTACAAGACAAGCAATGCCA
GAAGAATTGGCTGAACAGGTAGACCTTTTAAAAGAAATTATTGAGGGCTTTAATATAAAGATTTTAGAATTAGAAGGTTA
CGAAGCTGATGACATCATAGGTACTATTTCAAAGTTGGCAGAGGAAAAAGAAATGGAAGTGCTTGTAGTTACAGGAGATA
GGGACGCGCTTCAATTAGTTTCGGATAAAGTGAAAGTGAAGATTTCTAAAAAGGGTATAACTCAGATGGAAGAGTTTGAC
GAAAAGGCTGTTTTAGAAAGATATGAAATAACTCCTCACCAATTTATAGATTTAAAAGGACTAATGGGGGATAAATCTGA
CAACATCCCTGGAATACCTAATATAGGGGAAAAAACAGCAATTAAACTATTAAAAGACTTTGGAACAATTGAGAATTTAC
TACAAAATCTTTCTCAGCTCAAAGGTAAGATAAAAGAAAATATAGAAAATAATAAAGAATTAGCTATAATGAGTAAAAAA
CTTGTCACTATAAAAAGAGACATTCCCATTGAGATAGATTTTGAGGAATATAGAGTAAAAGAGTTTAATGAGGAGAAGCT
TTTAGAGATTTTTAATAAATTAGAATTCTTTAGTTTGATTGATAGCATAAAGAAAAAAAATGACGTAGAGATTGTAAATA
ATCATAAAGTTCAAAAATGGTCAAAAGTAGATATAAAAAAATTAATAGCTTTATTGCAAGATAGCAAAAGTATTGCTTTT
TATCCACTAATTTATGAAGGGGAAATAAAGAAAATAGCTTTTTCTTTTGGAAACGATACTGTTTATATTGATGGTTTTCA
AATAAAAGATTTAAAAGAGATTTTTGAAAAAGAAAAATTTGAATTTACAACCCATGAAATAAAAGATTTTTTAGTTAAGC
TTTCTTATAAAGGAATAGAGTGTAAAAGCAAGTACATGGATACTGCTATAATGGCTTATCTTTTAAATCCTTCTGAGTCT
AACTATGATTTAGATCGTGTGCTAAAAAAATATTTAAAGGTTGATGTTCCATCTTATGAAGAGGTATTTGGCAAAGGTAG
GGATAAAAAGAAACTTGAAGAAATAGGAGAAGATATACTTGCTGATTACATTTGCAGTAGATGTGTACATCTATTTGATT
TAAGAGAAAAGTTGATGAATTTTATTGAAGAAATGGATATGAAAAGACTTTTGTTGGAAATAGAAATGCCTCTTGTAGAA
GTCTTAAAATCAATGGAAGTAAGTGGTTTTACATTGGATAAAGAAGTCCTAAAAGAGCTTTCACAAAAAATAAATGATAG
AATAGCAGAAATACTAGATAAAATTTATAAAGAGGCAGGGTATCAATTTAATGTAAATTCTCCTAAGCAATTAAGTGAAT
TTTTGTTTGAAAAATTAAATTTACCAGTAATAAAGAAAACAAAAACAGGGTATTCTACAGATTCTGAAGTTTTAGAGCAA
TTAGTTCCTTACAATAATATTGTCAATGATATAATAGAGTATAGGCAACTTACAAAACTTAAATCTACTTATATAGATGG
ATTTTTGCCTCTCATGGATGAAAACAATAGAGTACATTCTAATTTTAAGCAAATGGTCACTTCTACAGGCAGAATAAGCA
GTACCGAGCCAAATCTACAAAATATACCTATAAGAGAAGAATTTGGAAGGCAAATTAGAAGAGCTTTTATTCCGCGGACT
AAAGATGGGTATATTGTCTCAGCTGATTATTCTCAGATTGAACTACGAGTTTTAGCACATGTTTCGGGAGATGAAAAGCT
AATAGAATCTTTTATGAATAATGAAGATATACATTTAAGGACTGCTTCGGAAGTTTTTAAAGTCCCAATGGAAAAAGTTA
CACCAGAGATGAGAAGAGTAGCAAAAGCCGTAAACTTTGGCATAATATATGGCATCAGCGATTACGGGCTTTCTCGAGAC
CTTAAAATATCAAGAAAAGAGGCAAAAGAGTATATAAATAATTATTTTGAAAGATACAAAGGAGTAAAAGAATATATTGA
AAAAATAGTTCGATTTGCAAAAGAAAATGGCTATGTGATTACAATAATGAACAGGAGAAGATATATTCCTGAGATAAACT
CCAGAAATTTTACTCAAAGGTCACAGGCTGAAAGGTTAGCGATGAATGCTCCAATACAGGGAAGTGCTGCCGATATAATA
AAAATGGCAATGGTTAGAGTGTACAACGATTTAGAAAAATTAAAGCTTAAGTCTAAGCTTATATTACAAGTTCATGACGA
ACTTGTAGTGGATACTTATAAAGATGAAGTAGAAATCGTAAAAAAGATACTCAAAGATAATATGGAAAATGTAGTACAAT
TAAAAGTTCCCCTTGTAGTGGAAATTGGAGTAGGACCCAATTGGTTTTTAGCCAAGTGA

Upstream 100 bases:

>100_bases
AAAGTGTAATTTCTCAGATCAAAAAAAGAAGATGACAATTTACATCTTCTTTTTTTTGCGGTATATTAAAATACAAAGAA
ATAAAAATGGGAGTGATTTA

Downstream 100 bases:

>100_bases
GGTGTACAAAATGCAAGTAATTGGACTGACTGGTGGCATTGCGTCTGGGAAAAGCACTGTTTCTAAGCTTTTAAAGAAGA
TGGGGGCTGTGGTTATTGAT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 872; Mature: 871

Protein sequence:

>872_residues
MSKFLVIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLIEEEKPDYIAIAFDKKAPTFRHKEYQDYKATRQAMP
EELAEQVDLLKEIIEGFNIKILELEGYEADDIIGTISKLAEEKEMEVLVVTGDRDALQLVSDKVKVKISKKGITQMEEFD
EKAVLERYEITPHQFIDLKGLMGDKSDNIPGIPNIGEKTAIKLLKDFGTIENLLQNLSQLKGKIKENIENNKELAIMSKK
LVTIKRDIPIEIDFEEYRVKEFNEEKLLEIFNKLEFFSLIDSIKKKNDVEIVNNHKVQKWSKVDIKKLIALLQDSKSIAF
YPLIYEGEIKKIAFSFGNDTVYIDGFQIKDLKEIFEKEKFEFTTHEIKDFLVKLSYKGIECKSKYMDTAIMAYLLNPSES
NYDLDRVLKKYLKVDVPSYEEVFGKGRDKKKLEEIGEDILADYICSRCVHLFDLREKLMNFIEEMDMKRLLLEIEMPLVE
VLKSMEVSGFTLDKEVLKELSQKINDRIAEILDKIYKEAGYQFNVNSPKQLSEFLFEKLNLPVIKKTKTGYSTDSEVLEQ
LVPYNNIVNDIIEYRQLTKLKSTYIDGFLPLMDENNRVHSNFKQMVTSTGRISSTEPNLQNIPIREEFGRQIRRAFIPRT
KDGYIVSADYSQIELRVLAHVSGDEKLIESFMNNEDIHLRTASEVFKVPMEKVTPEMRRVAKAVNFGIIYGISDYGLSRD
LKISRKEAKEYINNYFERYKGVKEYIEKIVRFAKENGYVITIMNRRRYIPEINSRNFTQRSQAERLAMNAPIQGSAADII
KMAMVRVYNDLEKLKLKSKLILQVHDELVVDTYKDEVEIVKKILKDNMENVVQLKVPLVVEIGVGPNWFLAK

Sequences:

>Translated_872_residues
MSKFLVIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLIEEEKPDYIAIAFDKKAPTFRHKEYQDYKATRQAMP
EELAEQVDLLKEIIEGFNIKILELEGYEADDIIGTISKLAEEKEMEVLVVTGDRDALQLVSDKVKVKISKKGITQMEEFD
EKAVLERYEITPHQFIDLKGLMGDKSDNIPGIPNIGEKTAIKLLKDFGTIENLLQNLSQLKGKIKENIENNKELAIMSKK
LVTIKRDIPIEIDFEEYRVKEFNEEKLLEIFNKLEFFSLIDSIKKKNDVEIVNNHKVQKWSKVDIKKLIALLQDSKSIAF
YPLIYEGEIKKIAFSFGNDTVYIDGFQIKDLKEIFEKEKFEFTTHEIKDFLVKLSYKGIECKSKYMDTAIMAYLLNPSES
NYDLDRVLKKYLKVDVPSYEEVFGKGRDKKKLEEIGEDILADYICSRCVHLFDLREKLMNFIEEMDMKRLLLEIEMPLVE
VLKSMEVSGFTLDKEVLKELSQKINDRIAEILDKIYKEAGYQFNVNSPKQLSEFLFEKLNLPVIKKTKTGYSTDSEVLEQ
LVPYNNIVNDIIEYRQLTKLKSTYIDGFLPLMDENNRVHSNFKQMVTSTGRISSTEPNLQNIPIREEFGRQIRRAFIPRT
KDGYIVSADYSQIELRVLAHVSGDEKLIESFMNNEDIHLRTASEVFKVPMEKVTPEMRRVAKAVNFGIIYGISDYGLSRD
LKISRKEAKEYINNYFERYKGVKEYIEKIVRFAKENGYVITIMNRRRYIPEINSRNFTQRSQAERLAMNAPIQGSAADII
KMAMVRVYNDLEKLKLKSKLILQVHDELVVDTYKDEVEIVKKILKDNMENVVQLKVPLVVEIGVGPNWFLAK
>Mature_871_residues
SKFLVIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLIEEEKPDYIAIAFDKKAPTFRHKEYQDYKATRQAMPE
ELAEQVDLLKEIIEGFNIKILELEGYEADDIIGTISKLAEEKEMEVLVVTGDRDALQLVSDKVKVKISKKGITQMEEFDE
KAVLERYEITPHQFIDLKGLMGDKSDNIPGIPNIGEKTAIKLLKDFGTIENLLQNLSQLKGKIKENIENNKELAIMSKKL
VTIKRDIPIEIDFEEYRVKEFNEEKLLEIFNKLEFFSLIDSIKKKNDVEIVNNHKVQKWSKVDIKKLIALLQDSKSIAFY
PLIYEGEIKKIAFSFGNDTVYIDGFQIKDLKEIFEKEKFEFTTHEIKDFLVKLSYKGIECKSKYMDTAIMAYLLNPSESN
YDLDRVLKKYLKVDVPSYEEVFGKGRDKKKLEEIGEDILADYICSRCVHLFDLREKLMNFIEEMDMKRLLLEIEMPLVEV
LKSMEVSGFTLDKEVLKELSQKINDRIAEILDKIYKEAGYQFNVNSPKQLSEFLFEKLNLPVIKKTKTGYSTDSEVLEQL
VPYNNIVNDIIEYRQLTKLKSTYIDGFLPLMDENNRVHSNFKQMVTSTGRISSTEPNLQNIPIREEFGRQIRRAFIPRTK
DGYIVSADYSQIELRVLAHVSGDEKLIESFMNNEDIHLRTASEVFKVPMEKVTPEMRRVAKAVNFGIIYGISDYGLSRDL
KISRKEAKEYINNYFERYKGVKEYIEKIVRFAKENGYVITIMNRRRYIPEINSRNFTQRSQAERLAMNAPIQGSAADIIK
MAMVRVYNDLEKLKLKSKLILQVHDELVVDTYKDEVEIVKKILKDNMENVVQLKVPLVVEIGVGPNWFLAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=516, Percent_Identity=29.2635658914729, Blast_Score=241, Evalue=2e-63,
Organism=Homo sapiens, GI139394648, Length=621, Percent_Identity=26.2479871175523, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1790294, Length=927, Percent_Identity=38.7270765911543, Blast_Score=577, Evalue=1e-165,
Organism=Escherichia coli, GI226510970, Length=221, Percent_Identity=27.1493212669683, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI86563272, Length=414, Percent_Identity=29.7101449275362, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI17933644, Length=586, Percent_Identity=29.8634812286689, Blast_Score=243, Evalue=4e-64,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 101294; Mature: 101163

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFLVIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLIEEEKPDYIAIAFDKK
CCCEEEEECCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
APTFRHKEYQDYKATRQAMPEELAEQVDLLKEIIEGFNIKILELEGYEADDIIGTISKLA
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
EEKEMEVLVVTGDRDALQLVSDKVKVKISKKGITQMEEFDEKAVLERYEITPHQFIDLKG
CCCCCEEEEEECCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHEEHHH
LMGDKSDNIPGIPNIGEKTAIKLLKDFGTIENLLQNLSQLKGKIKENIENNKELAIMSKK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
LVTIKRDIPIEIDFEEYRVKEFNEEKLLEIFNKLEFFSLIDSIKKKNDVEIVNNHKVQKW
HHEEECCCCEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHH
SKVDIKKLIALLQDSKSIAFYPLIYEGEIKKIAFSFGNDTVYIDGFQIKDLKEIFEKEKF
HHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEECCCCEEEECCEEHHHHHHHHHHHCC
EFTTHEIKDFLVKLSYKGIECKSKYMDTAIMAYLLNPSESNYDLDRVLKKYLKVDVPSYE
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCCCCHH
EVFGKGRDKKKLEEIGEDILADYICSRCVHLFDLREKLMNFIEEMDMKRLLLEIEMPLVE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKSMEVSGFTLDKEVLKELSQKINDRIAEILDKIYKEAGYQFNVNSPKQLSEFLFEKLN
HHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCC
LPVIKKTKTGYSTDSEVLEQLVPYNNIVNDIIEYRQLTKLKSTYIDGFLPLMDENNRVHS
CCEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
NFKQMVTSTGRISSTEPNLQNIPIREEFGRQIRRAFIPRTKDGYIVSADYSQIELRVLAH
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEE
VSGDEKLIESFMNNEDIHLRTASEVFKVPMEKVTPEMRRVAKAVNFGIIYGISDYGLSRD
CCCCHHHHHHHCCCCCEEEEEHHHHHHCCHHHCCHHHHHHHHHHCCCEEEEECCCCCCCC
LKISRKEAKEYINNYFERYKGVKEYIEKIVRFAKENGYVITIMNRRRYIPEINSRNFTQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHH
SQAERLAMNAPIQGSAADIIKMAMVRVYNDLEKLKLKSKLILQVHDELVVDTYKDEVEIV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKILKDNMENVVQLKVPLVVEIGVGPNWFLAK
HHHHHHCHHHHEEEEECEEEEECCCCCCCCCC
>Mature Secondary Structure 
SKFLVIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLIEEEKPDYIAIAFDKK
CCEEEEECCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
APTFRHKEYQDYKATRQAMPEELAEQVDLLKEIIEGFNIKILELEGYEADDIIGTISKLA
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
EEKEMEVLVVTGDRDALQLVSDKVKVKISKKGITQMEEFDEKAVLERYEITPHQFIDLKG
CCCCCEEEEEECCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHEEHHH
LMGDKSDNIPGIPNIGEKTAIKLLKDFGTIENLLQNLSQLKGKIKENIENNKELAIMSKK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
LVTIKRDIPIEIDFEEYRVKEFNEEKLLEIFNKLEFFSLIDSIKKKNDVEIVNNHKVQKW
HHEEECCCCEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHH
SKVDIKKLIALLQDSKSIAFYPLIYEGEIKKIAFSFGNDTVYIDGFQIKDLKEIFEKEKF
HHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEECCCCEEEECCEEHHHHHHHHHHHCC
EFTTHEIKDFLVKLSYKGIECKSKYMDTAIMAYLLNPSESNYDLDRVLKKYLKVDVPSYE
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCCCCHH
EVFGKGRDKKKLEEIGEDILADYICSRCVHLFDLREKLMNFIEEMDMKRLLLEIEMPLVE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKSMEVSGFTLDKEVLKELSQKINDRIAEILDKIYKEAGYQFNVNSPKQLSEFLFEKLN
HHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCC
LPVIKKTKTGYSTDSEVLEQLVPYNNIVNDIIEYRQLTKLKSTYIDGFLPLMDENNRVHS
CCEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
NFKQMVTSTGRISSTEPNLQNIPIREEFGRQIRRAFIPRTKDGYIVSADYSQIELRVLAH
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEE
VSGDEKLIESFMNNEDIHLRTASEVFKVPMEKVTPEMRRVAKAVNFGIIYGISDYGLSRD
CCCCHHHHHHHCCCCCEEEEEHHHHHHCCHHHCCHHHHHHHHHHCCCEEEEECCCCCCCC
LKISRKEAKEYINNYFERYKGVKEYIEKIVRFAKENGYVITIMNRRRYIPEINSRNFTQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHH
SQAERLAMNAPIQGSAADIIKMAMVRVYNDLEKLKLKSKLILQVHDELVVDTYKDEVEIV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKILKDNMENVVQLKVPLVVEIGVGPNWFLAK
HHHHHHCHHHHEEEEECEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]