Click here to switch to the map view.

The map label for this gene is pyrB [H]

Identifier: 167040451

GI number: 167040451

Start: 1828392

End: 1829306

Strand: Reverse

Name: pyrB [H]

Synonym: Teth514_1818

Alternate gene names: 167040451

Gene position: 1829306-1828392 (Counterclockwise)

Preceding gene: 167040452

Following gene: 167040450

Centisome position: 74.44

GC content: 39.56

Gene sequence:

>915_bases
GTGAAGAGAAAAGACCTGTTAGGCATTAAGGATTTATCGAAAGAAGAAATACTAGAAGTTTTAGACACTGCAAAGGAGAT
GAAAAAAATTCTAAATGGAGAAAAATATTCACAGATACTAAAGGGAAAGACAGTTGTGACGATTTTTTTCGAACCTAGCA
CGAGGACAAGACTATCTTTTGAGATGGCAGCAAAGTATTTAGGAGCCCATTACGGCAATATAGAAGTGGCAACCAGCAGT
GTGGCTAAAGGGGAGTCTTTAATAGATACCATAAGGACTGTTGAGATGATGAAGGCGGATGTGATAATAACAAGACACAA
CATGAGTGGAGCGCCGCATCTTATGGCAAAATACACAAATGCCTCTATCATAAACGCAGGAGATGGTATAAATGAACATC
CCACCCAAGCCTTGTTGGACATGATGACAATCAGAGAAAAGAAAGAAAGCTTTGAAGGATTAAAAGTGGCAATAATAGGA
GATATTATGCACAGCAGAGTTGCACGGTCAAATATATGGGGACTTAAAAAGATGGGGGCGGAAGTGAGAGTTGCAGGCCC
TTCAACCTTGATGCCACCTGCAATAGAAAAATTAGGAGTCAAGGCTTACTACAATGTAGAGGAAGCAATAGAAGGAGCAG
ATGTGGTGATGGGGCTTAGAATACAGCTGGAAAGACAAAAAAGCGGTCTTTTTCCCTCAATTGACGAATATAGAGAGTAT
TTTGGGATAAATAAGGAAAGAATGAAACTTGCAAAACCTGATGCGATATTGATGCATCCAGGGCCTATAAACAGAAATGT
TGAACTTACGTCAGAAGCAGCAAATGACAAGTATTCTGTTATAGAAGAACAGGTGACAAACGGTGTCGCTGTCAGGATGG
CACTTTTAAAAATACTTTGTGAAAGGAGAAATTAA

Upstream 100 bases:

>100_bases
CTAATTTTAACAAAAGAGAATATTTTATACAAAAAGAGTAGTACCATTTCACACTATTGTGATGTGGTTACTACTCTTTT
TTTAAAAGGAGGAAGGTAAT

Downstream 100 bases:

>100_bases
AATGAGGATGATTATAAAAAACGGTACGGTAATAGATGGATTTGGAAGTGAAGCCACAGCGGATATATTGATAGACTATG
GTATCATAAAAGCGATTGAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKRKDLLGIKDLSKEEILEVLDTAKEMKKILNGEKYSQILKGKTVVTIFFEPSTRTRLSFEMAAKYLGAHYGNIEVATSS
VAKGESLIDTIRTVEMMKADVIITRHNMSGAPHLMAKYTNASIINAGDGINEHPTQALLDMMTIREKKESFEGLKVAIIG
DIMHSRVARSNIWGLKKMGAEVRVAGPSTLMPPAIEKLGVKAYYNVEEAIEGADVVMGLRIQLERQKSGLFPSIDEYREY
FGINKERMKLAKPDAILMHPGPINRNVELTSEAANDKYSVIEEQVTNGVAVRMALLKILCERRN

Sequences:

>Translated_304_residues
MKRKDLLGIKDLSKEEILEVLDTAKEMKKILNGEKYSQILKGKTVVTIFFEPSTRTRLSFEMAAKYLGAHYGNIEVATSS
VAKGESLIDTIRTVEMMKADVIITRHNMSGAPHLMAKYTNASIINAGDGINEHPTQALLDMMTIREKKESFEGLKVAIIG
DIMHSRVARSNIWGLKKMGAEVRVAGPSTLMPPAIEKLGVKAYYNVEEAIEGADVVMGLRIQLERQKSGLFPSIDEYREY
FGINKERMKLAKPDAILMHPGPINRNVELTSEAANDKYSVIEEQVTNGVAVRMALLKILCERRN
>Mature_304_residues
MKRKDLLGIKDLSKEEILEVLDTAKEMKKILNGEKYSQILKGKTVVTIFFEPSTRTRLSFEMAAKYLGAHYGNIEVATSS
VAKGESLIDTIRTVEMMKADVIITRHNMSGAPHLMAKYTNASIINAGDGINEHPTQALLDMMTIREKKESFEGLKVAIIG
DIMHSRVARSNIWGLKKMGAEVRVAGPSTLMPPAIEKLGVKAYYNVEEAIEGADVVMGLRIQLERQKSGLFPSIDEYREY
FGINKERMKLAKPDAILMHPGPINRNVELTSEAANDKYSVIEEQVTNGVAVRMALLKILCERRN

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=36.8932038834951, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI38788445, Length=200, Percent_Identity=33, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=32.5878594249201, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1790703, Length=284, Percent_Identity=26.4084507042254, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=25.748502994012, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=328, Percent_Identity=32.9268292682927, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=33.5483870967742, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6322373, Length=334, Percent_Identity=26.0479041916168, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=33.4394904458599, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33926; Mature: 33926

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKDLLGIKDLSKEEILEVLDTAKEMKKILNGEKYSQILKGKTVVTIFFEPSTRTRLSF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCCHHHH
EMAAKYLGAHYGNIEVATSSVAKGESLIDTIRTVEMMKADVIITRHNMSGAPHLMAKYTN
HHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHEEECC
ASIINAGDGINEHPTQALLDMMTIREKKESFEGLKVAIIGDIMHSRVARSNIWGLKKMGA
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCHHHCCC
EVRVAGPSTLMPPAIEKLGVKAYYNVEEAIEGADVVMGLRIQLERQKSGLFPSIDEYREY
EEEECCCCCCCCHHHHHCCCHHEECHHHHHCCCCEEEEEEEEEEHHHCCCCCCHHHHHHH
FGINKERMKLAKPDAILMHPGPINRNVELTSEAANDKYSVIEEQVTNGVAVRMALLKILC
HCCCHHHHHHCCCCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
ERRN
HCCC
>Mature Secondary Structure
MKRKDLLGIKDLSKEEILEVLDTAKEMKKILNGEKYSQILKGKTVVTIFFEPSTRTRLSF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCCHHHH
EMAAKYLGAHYGNIEVATSSVAKGESLIDTIRTVEMMKADVIITRHNMSGAPHLMAKYTN
HHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHEEECC
ASIINAGDGINEHPTQALLDMMTIREKKESFEGLKVAIIGDIMHSRVARSNIWGLKKMGA
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCHHHCCC
EVRVAGPSTLMPPAIEKLGVKAYYNVEEAIEGADVVMGLRIQLERQKSGLFPSIDEYREY
EEEECCCCCCCCHHHHHCCCHHEECHHHHHCCCCEEEEEEEEEEHHHCCCCCCHHHHHHH
FGINKERMKLAKPDAILMHPGPINRNVELTSEAANDKYSVIEEQVTNGVAVRMALLKILC
HCCCHHHHHHCCCCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHH
ERRN
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]