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The map label for this gene is hisZ [H]

Identifier: 167039649

GI number: 167039649

Start: 1029956

End: 1031098

Strand: Direct

Name: hisZ [H]

Synonym: Teth514_0999

Alternate gene names: 167039649

Gene position: 1029956-1031098 (Clockwise)

Preceding gene: 167039645

Following gene: 167039650

Centisome position: 41.91

GC content: 32.9

Gene sequence:

>1143_bases
ATGATATATTTGCCTGATGGTGTACAAGATTTTTTGCCAGAGGAATATAAGTTTAAGCGCAAAATAGAAGAAAAGTTTAG
AGAGGTTTTTATAAAATTTGGCTATCAAGAGATAATGCCTCCTACTTTTGAATATAACGATAATTTTTCTGTTTTGTTTG
ATGAAAACAATTTGTACAGATTTTTCGATAAAAAGGGAAATATATTAGCTTTAAGGCCAGATGTGACTACTCAAATAGCG
AGAATTGTGTCTACAAAGTTAAAAGGTAGTTTCCCACTTAAGTTATGCTATGTCGCTAATGTCTATCGCTATGATGATCC
CCAGGTAGGAAAAATGAGAGAGTTTACTCAGGCAGGGATTGAACTTATAGGTACAAATCATGAAGAATCTGATGCAGAGA
TAATAGCAGTTGCGATAGAGGCTTTAAAAAGTATTGGAATAGAGGACTTTAAAGTGGACGTGGGACAGGTGGAATTTTTT
AAAAGAGTAGTGGAAGATTTAGAGTTACAAAATGAAGAAATAAACCTTTTAAGAGAGTTTTTGCAACAGAAAAATCAATC
TGCGATAGAAGAATTCATAAATGATAAAGGAATTAGGGGCAAAAAAGCAAAGTTTTTAAGTGAACTTCCTCTTTTATTTG
GTGGCGAAGAAGTGCTTAAAAGAGCAAAAGAGCTTTATGATACTCCTAAACTAGGTGAAACGATAGATTACCTTGAAAGA
GTATATAGAATATTAAAAGATTTTGGTATGGAAGAGTATATAAGTTTTGACTTAGGAATGGTGCAAAATCTTAATTATTA
TACTGGTATCATATTCAGGTGTTTTGTTAAAGGGATTGGTTATGCTATATGTACCGGAGGAAGGTATGATGGTCTTTTAG
GGATTTTTGGAGAGCACATTCCGGCGACAGGTTTTGCTATAAGTGTGGAAAGGTCAATGTTAGCACTTCAAAAACAGAAA
AAAGATATTATTGATAAATCCTGGAGAGTTTTAATAAAATACAAGGAAAACTTAAGAAGTAATGCTTACAAGAAAGCCAC
TTTGTTAAGAGGTGAGGGAAAAATTGTAGAGATGTATAGCTATGAAAAAGCGAAGCATGTTGATGAAAATAGCTTTGACG
AAATTATAGATATGGAGGAATAA

Upstream 100 bases:

>100_bases
GTCTGTGCTTGACATACATTGAAAATTAGGATACAATAAAAGAAATACAGTTTATTACTTTATCACGTTACAAAGACAGA
GTAAATTGTGGGGTGTTTTA

Downstream 100 bases:

>100_bases
TATGGAAAATATTTCTATTGCACTGCCAAAAGGTAGAATGGCAGATAGTGCTATAACTTTGTTTGAAAAGGCAGGAATTG
CTGAGAATATTTTAAAAGAT

Product: histidine--tRNA ligase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIYLPDGVQDFLPEEYKFKRKIEEKFREVFIKFGYQEIMPPTFEYNDNFSVLFDENNLYRFFDKKGNILALRPDVTTQIA
RIVSTKLKGSFPLKLCYVANVYRYDDPQVGKMREFTQAGIELIGTNHEESDAEIIAVAIEALKSIGIEDFKVDVGQVEFF
KRVVEDLELQNEEINLLREFLQQKNQSAIEEFINDKGIRGKKAKFLSELPLLFGGEEVLKRAKELYDTPKLGETIDYLER
VYRILKDFGMEEYISFDLGMVQNLNYYTGIIFRCFVKGIGYAICTGGRYDGLLGIFGEHIPATGFAISVERSMLALQKQK
KDIIDKSWRVLIKYKENLRSNAYKKATLLRGEGKIVEMYSYEKAKHVDENSFDEIIDMEE

Sequences:

>Translated_380_residues
MIYLPDGVQDFLPEEYKFKRKIEEKFREVFIKFGYQEIMPPTFEYNDNFSVLFDENNLYRFFDKKGNILALRPDVTTQIA
RIVSTKLKGSFPLKLCYVANVYRYDDPQVGKMREFTQAGIELIGTNHEESDAEIIAVAIEALKSIGIEDFKVDVGQVEFF
KRVVEDLELQNEEINLLREFLQQKNQSAIEEFINDKGIRGKKAKFLSELPLLFGGEEVLKRAKELYDTPKLGETIDYLER
VYRILKDFGMEEYISFDLGMVQNLNYYTGIIFRCFVKGIGYAICTGGRYDGLLGIFGEHIPATGFAISVERSMLALQKQK
KDIIDKSWRVLIKYKENLRSNAYKKATLLRGEGKIVEMYSYEKAKHVDENSFDEIIDMEE
>Mature_380_residues
MIYLPDGVQDFLPEEYKFKRKIEEKFREVFIKFGYQEIMPPTFEYNDNFSVLFDENNLYRFFDKKGNILALRPDVTTQIA
RIVSTKLKGSFPLKLCYVANVYRYDDPQVGKMREFTQAGIELIGTNHEESDAEIIAVAIEALKSIGIEDFKVDVGQVEFF
KRVVEDLELQNEEINLLREFLQQKNQSAIEEFINDKGIRGKKAKFLSELPLLFGGEEVLKRAKELYDTPKLGETIDYLER
VYRILKDFGMEEYISFDLGMVQNLNYYTGIIFRCFVKGIGYAICTGGRYDGLLGIFGEHIPATGFAISVERSMLALQKQK
KDIIDKSWRVLIKYKENLRSNAYKKATLLRGEGKIVEMYSYEKAKHVDENSFDEIIDMEE

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=337, Percent_Identity=27.5964391691395, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI15029520, Length=341, Percent_Identity=25.2199413489736, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1788861, Length=326, Percent_Identity=27.6073619631902, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71993693, Length=397, Percent_Identity=21.9143576826196, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71993686, Length=397, Percent_Identity=21.9143576826196, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325290, Length=361, Percent_Identity=25.7617728531856, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24643061, Length=345, Percent_Identity=24.9275362318841, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24643059, Length=364, Percent_Identity=24.4505494505494, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 44163; Mature: 44163

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLPDGVQDFLPEEYKFKRKIEEKFREVFIKFGYQEIMPPTFEYNDNFSVLFDENNLYR
CEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEECCCCEEEEEECCCEEE
FFDKKGNILALRPDVTTQIARIVSTKLKGSFPLKLCYVANVYRYDDPQVGKMREFTQAGI
EECCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHCC
ELIGTNHEESDAEIIAVAIEALKSIGIEDFKVDVGQVEFFKRVVEDLELQNEEINLLREF
EEECCCCCCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LQQKNQSAIEEFINDKGIRGKKAKFLSELPLLFGGEEVLKRAKELYDTPKLGETIDYLER
HHHHCHHHHHHHHCCCCCCCHHHHHHHHCCHHCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VYRILKDFGMEEYISFDLGMVQNLNYYTGIIFRCFVKGIGYAICTGGRYDGLLGIFGEHI
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHCCC
PATGFAISVERSMLALQKQKKDIIDKSWRVLIKYKENLRSNAYKKATLLRGEGKIVEMYS
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEC
YEKAKHVDENSFDEIIDMEE
CHHHHCCCCCCHHHHCCCCC
>Mature Secondary Structure
MIYLPDGVQDFLPEEYKFKRKIEEKFREVFIKFGYQEIMPPTFEYNDNFSVLFDENNLYR
CEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEECCCCEEEEEECCCEEE
FFDKKGNILALRPDVTTQIARIVSTKLKGSFPLKLCYVANVYRYDDPQVGKMREFTQAGI
EECCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHCC
ELIGTNHEESDAEIIAVAIEALKSIGIEDFKVDVGQVEFFKRVVEDLELQNEEINLLREF
EEECCCCCCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LQQKNQSAIEEFINDKGIRGKKAKFLSELPLLFGGEEVLKRAKELYDTPKLGETIDYLER
HHHHCHHHHHHHHCCCCCCCHHHHHHHHCCHHCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VYRILKDFGMEEYISFDLGMVQNLNYYTGIIFRCFVKGIGYAICTGGRYDGLLGIFGEHI
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHCCC
PATGFAISVERSMLALQKQKKDIIDKSWRVLIKYKENLRSNAYKKATLLRGEGKIVEMYS
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEC
YEKAKHVDENSFDEIIDMEE
CHHHHCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA