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The map label for this gene is greA

Identifier: 167039463

GI number: 167039463

Start: 842047

End: 842520

Strand: Direct

Name: greA

Synonym: Teth514_0809

Alternate gene names: 167039463

Gene position: 842047-842520 (Clockwise)

Preceding gene: 167039462

Following gene: 167039464

Centisome position: 34.27

GC content: 34.81

Gene sequence:

>474_bases
ATGAGCAAGCCTGTAATTTTAACTTATGAAGGACTCAAAAAGTTAGAGGAAGAATTAGAATATTTAAAGACGGTCAAAAG
AGCTGAAGTAGCAGAAAAGATAAAACAAGCGAGAGCATTTGGAGATTTAAGTGAAAATTCAGAATACGACGAAGCAAAAA
ACGAACAAGCGTTTATAGAAGGAAGAATAGCTACTCTGGAAGCCATGCTTAAAAATGCAAAAGTGATTGACGAAGAAGAC
ATAAAACTTGACCAGGTTAGCATTGGATGTACTGTAAAAGTGTATGACGAATCCTATAATGAAGAAGTAGAGTATACAAT
TGTAGGTTCAGCAGAAGCTGACCCAATGAATAACAAGATATCTGACGAATCTCCAATAGGGAAAGCGCTTTTAGGGAAAA
AAGTAGGAGATGTTGTCTCTGTTGAAGTTCCTGCTGGAATTATAAAGTTAAAAATATTAGAAATACGCAAATAA

Upstream 100 bases:

>100_bases
GCTGAAGCAGTGCTTGTGTATTAATATTAACATAAATTTCATTATTGTTACATATGAATAAAATACAATTTTCAATATTT
TATAAAAAGGAGAATCATCT

Downstream 100 bases:

>100_bases
AGGGTGATGGAGGTATAATATGTCTAATTCAAATGATAACATTTGGAACAACACTGAAGAACTCAATGAACTTTTGCGAA
TAAGAAGAGAAAAATTAAAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MSKPVILTYEGLKKLEEELEYLKTVKRAEVAEKIKQARAFGDLSENSEYDEAKNEQAFIEGRIATLEAMLKNAKVIDEED
IKLDQVSIGCTVKVYDESYNEEVEYTIVGSAEADPMNNKISDESPIGKALLGKKVGDVVSVEVPAGIIKLKILEIRK

Sequences:

>Translated_157_residues
MSKPVILTYEGLKKLEEELEYLKTVKRAEVAEKIKQARAFGDLSENSEYDEAKNEQAFIEGRIATLEAMLKNAKVIDEED
IKLDQVSIGCTVKVYDESYNEEVEYTIVGSAEADPMNNKISDESPIGKALLGKKVGDVVSVEVPAGIIKLKILEIRK
>Mature_156_residues
SKPVILTYEGLKKLEEELEYLKTVKRAEVAEKIKQARAFGDLSENSEYDEAKNEQAFIEGRIATLEAMLKNAKVIDEEDI
KLDQVSIGCTVKVYDESYNEEVEYTIVGSAEADPMNNKISDESPIGKALLGKKVGDVVSVEVPAGIIKLKILEIRK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=42.2077922077922, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI87082261, Length=146, Percent_Identity=35.6164383561644, Blast_Score=81, Evalue=4e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_THEP3 (B0KCE3)

Other databases:

- EMBL:   CP000924
- RefSeq:   YP_001664322.1
- ProteinModelPortal:   B0KCE3
- SMR:   B0KCE3
- GeneID:   5874241
- GenomeReviews:   CP000924_GR
- KEGG:   tpd:Teth39_0317
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   TPSE340099:TETH39_0317-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17481; Mature: 17350

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPVILTYEGLKKLEEELEYLKTVKRAEVAEKIKQARAFGDLSENSEYDEAKNEQAFIE
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHH
GRIATLEAMLKNAKVIDEEDIKLDQVSIGCTVKVYDESYNEEVEYTIVGSAEADPMNNKI
HHHHHHHHHHHCCCCCCCCCCEEEEEEECEEEEEECCCCCCCEEEEEEECCCCCCCCCCC
SDESPIGKALLGKKVGDVVSVEVPAGIIKLKILEIRK
CCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECC
>Mature Secondary Structure 
SKPVILTYEGLKKLEEELEYLKTVKRAEVAEKIKQARAFGDLSENSEYDEAKNEQAFIE
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHH
GRIATLEAMLKNAKVIDEEDIKLDQVSIGCTVKVYDESYNEEVEYTIVGSAEADPMNNKI
HHHHHHHHHHHCCCCCCCCCCEEEEEEECEEEEEECCCCCCCEEEEEEECCCCCCCCCCC
SDESPIGKALLGKKVGDVVSVEVPAGIIKLKILEIRK
CCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA