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The map label for this gene is thiE [H]

Identifier: 167039225

GI number: 167039225

Start: 590651

End: 591286

Strand: Direct

Name: thiE [H]

Synonym: Teth514_0566

Alternate gene names: 167039225

Gene position: 590651-591286 (Clockwise)

Preceding gene: 167039224

Following gene: 167039226

Centisome position: 24.04

GC content: 38.21

Gene sequence:

>636_bases
ATGGACTTAACCCTTTATGCTATAACGGATAGGTCTTACATAAAGAATATGGATATTGCTGAAGCAGTAGAACTAGCGAT
AAAAGGCGGTGCAACAGTAATTCAACTGAGAGAAAAAGATATATCAAGTAGGGAATTTTATGAAATTGCTCTCAAAGTTA
AAGAAGTGACAAAAAGAAATAGAATCCCTCTCATTATTAACGACAGAGTGGATATAGCTTTGGCAGTAGATGCAGATGGA
GTTCACGTTGGACAGGAGGATTTGCCGGCAGATATTGTAAGAAAAATAATAGGTCGTGATAAAATAGTAGGGGTATCAGC
GAGAACAGTGGAAGAAGCTTTAAAGGCACAAAGGGATGGAGCGGATTATTTAGGGGTAGGTGCGGTGTTTAAGACTCCAA
CAAAGCCGGAGGCAGAAGCCATTGGAATAGAAGGGTTAAAAAAAATAAAAGAGGCTGTTACTATTCCAGTCGTTGCAATA
GGAGGTATAACGAAGGATAATGCTTATGAAGTCATGTTAAAGTCGGGAGTAGACGGGATATCCTCAGTTTCAGCTGTATT
TTATGGGGATATAGAAAACAATACAAGAAAACTCTTGGAGGTTATAAAAAAAGCGATAAATGATAGGAGGATTTGA

Upstream 100 bases:

>100_bases
AGCATACATTACAGGGGCTATAAAACATTCGTTGGCCATAGGGCATGGAGTAGGTCCTACTAACCATTTTTGGAATATAG
AAATAAGGAAGGTGTGAGAT

Downstream 100 bases:

>100_bases
AATGAAAAAAGAATTGTTGAAAATTTTAAGGGAGAAAAAACCTTTAGTTCACCATATTACCAATGTAGTTACTGTCAACG
ATTGTGCTAATATAACACTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MDLTLYAITDRSYIKNMDIAEAVELAIKGGATVIQLREKDISSREFYEIALKVKEVTKRNRIPLIINDRVDIALAVDADG
VHVGQEDLPADIVRKIIGRDKIVGVSARTVEEALKAQRDGADYLGVGAVFKTPTKPEAEAIGIEGLKKIKEAVTIPVVAI
GGITKDNAYEVMLKSGVDGISSVSAVFYGDIENNTRKLLEVIKKAINDRRI

Sequences:

>Translated_211_residues
MDLTLYAITDRSYIKNMDIAEAVELAIKGGATVIQLREKDISSREFYEIALKVKEVTKRNRIPLIINDRVDIALAVDADG
VHVGQEDLPADIVRKIIGRDKIVGVSARTVEEALKAQRDGADYLGVGAVFKTPTKPEAEAIGIEGLKKIKEAVTIPVVAI
GGITKDNAYEVMLKSGVDGISSVSAVFYGDIENNTRKLLEVIKKAINDRRI
>Mature_211_residues
MDLTLYAITDRSYIKNMDIAEAVELAIKGGATVIQLREKDISSREFYEIALKVKEVTKRNRIPLIINDRVDIALAVDADG
VHVGQEDLPADIVRKIIGRDKIVGVSARTVEEALKAQRDGADYLGVGAVFKTPTKPEAEAIGIEGLKKIKEAVTIPVVAI
GGITKDNAYEVMLKSGVDGISSVSAVFYGDIENNTRKLLEVIKKAINDRRI

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=192, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=203, Percent_Identity=40.8866995073892, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23018; Mature: 23018

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTLYAITDRSYIKNMDIAEAVELAIKGGATVIQLREKDISSREFYEIALKVKEVTKRN
CCEEEEEEECCHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
RIPLIINDRVDIALAVDADGVHVGQEDLPADIVRKIIGRDKIVGVSARTVEEALKAQRDG
CCCEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHCCC
ADYLGVGAVFKTPTKPEAEAIGIEGLKKIKEAVTIPVVAIGGITKDNAYEVMLKSGVDGI
CCEEECCCEECCCCCCCHHHHCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHCCCCCH
SSVSAVFYGDIENNTRKLLEVIKKAINDRRI
HHHHEEEEECCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLTLYAITDRSYIKNMDIAEAVELAIKGGATVIQLREKDISSREFYEIALKVKEVTKRN
CCEEEEEEECCHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
RIPLIINDRVDIALAVDADGVHVGQEDLPADIVRKIIGRDKIVGVSARTVEEALKAQRDG
CCCEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHCCC
ADYLGVGAVFKTPTKPEAEAIGIEGLKKIKEAVTIPVVAIGGITKDNAYEVMLKSGVDGI
CCEEECCCEECCCCCCCHHHHCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHCCCCCH
SSVSAVFYGDIENNTRKLLEVIKKAINDRRI
HHHHEEEEECCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]