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The map label for this gene is thiM [H]

Identifier: 167039226

GI number: 167039226

Start: 591288

End: 592079

Strand: Direct

Name: thiM [H]

Synonym: Teth514_0567

Alternate gene names: 167039226

Gene position: 591288-592079 (Clockwise)

Preceding gene: 167039225

Following gene: 167039227

Centisome position: 24.06

GC content: 39.27

Gene sequence:

>792_bases
ATGAAAAAAGAATTGTTGAAAATTTTAAGGGAGAAAAAACCTTTAGTTCACCATATTACCAATGTAGTTACTGTCAACGA
TTGTGCTAATATAACACTGGCTATAGGAGCACTGCCAGTCATGGCCCATGCCTTGGAAGAAGTAGAAGAAATGGTAAGTG
CAGCAGATGCTTTAGTTTTAAATATAGGCACTCTTACAAATGAACAAGTAGAAGCGATGATAAAGGCAGGAAAGGCTGCA
AATAGATTTAAAGTACCAGTTATATTAGACCCTGTAGGAGCGGGAGCTACAAAGCTTAGGACACAAAGCTCAAAAAAGAT
ACTGGAAGAAGTAAAAATTTCTGTGATAAAAGGGAATAGTGCAGAGATTTCAATACTTGCGGGCAAAGGTGGAAAAATAC
GCGGCGTAGAGTCTCAAGGAGGAGTAGATGATATTGCTGAAGCTGCAAAAGATTTAGCAAACGCGTACAACGTTGTAGTA
GCAATGTCAGGTGCTACTGACATAATTACTGATGGAAAAAGAATTGCCTATGTGAAAAATGGACATCCTATGATGGGTAC
TATCACCGGCACAGGTTGTATGCTTACCTCTGTAGTAGCGAGCTTCTGCGGAGTATGTGAAGATTATTTTGAAACCACAA
TAGAAGCTTTTGTAGCTTTTGGAATAGCAGGTGAAAGAGCCGCTCAAAGCAGCAATGTCAAAGGGCCCGGCAGTTTTAAA
GTGACATTTTTTGATGAGATATATAATCTCACGCCGGAGATAATAGAAAAAGATAAGAAGGTGGAAGTATGA

Upstream 100 bases:

>100_bases
ATATCCTCAGTTTCAGCTGTATTTTATGGGGATATAGAAAACAATACAAGAAAACTCTTGGAGGTTATAAAAAAAGCGAT
AAATGATAGGAGGATTTGAA

Downstream 100 bases:

>100_bases
ATAAAATACATCGTATGGTTTTTATGGCACTTATTATTGCTATAGGCACCTTTACCTCTCAATATGTATGGTTTAGTGTA
GGTGTTGCCAAAGTTACACC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKKELLKILREKKPLVHHITNVVTVNDCANITLAIGALPVMAHALEEVEEMVSAADALVLNIGTLTNEQVEAMIKAGKAA
NRFKVPVILDPVGAGATKLRTQSSKKILEEVKISVIKGNSAEISILAGKGGKIRGVESQGGVDDIAEAAKDLANAYNVVV
AMSGATDIITDGKRIAYVKNGHPMMGTITGTGCMLTSVVASFCGVCEDYFETTIEAFVAFGIAGERAAQSSNVKGPGSFK
VTFFDEIYNLTPEIIEKDKKVEV

Sequences:

>Translated_263_residues
MKKELLKILREKKPLVHHITNVVTVNDCANITLAIGALPVMAHALEEVEEMVSAADALVLNIGTLTNEQVEAMIKAGKAA
NRFKVPVILDPVGAGATKLRTQSSKKILEEVKISVIKGNSAEISILAGKGGKIRGVESQGGVDDIAEAAKDLANAYNVVV
AMSGATDIITDGKRIAYVKNGHPMMGTITGTGCMLTSVVASFCGVCEDYFETTIEAFVAFGIAGERAAQSSNVKGPGSFK
VTFFDEIYNLTPEIIEKDKKVEV
>Mature_263_residues
MKKELLKILREKKPLVHHITNVVTVNDCANITLAIGALPVMAHALEEVEEMVSAADALVLNIGTLTNEQVEAMIKAGKAA
NRFKVPVILDPVGAGATKLRTQSSKKILEEVKISVIKGNSAEISILAGKGGKIRGVESQGGVDDIAEAAKDLANAYNVVV
AMSGATDIITDGKRIAYVKNGHPMMGTITGTGCMLTSVVASFCGVCEDYFETTIEAFVAFGIAGERAAQSSNVKGPGSFK
VTFFDEIYNLTPEIIEKDKKVEV

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=249, Percent_Identity=37.7510040160643, Blast_Score=157, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=270, Percent_Identity=33.7037037037037, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 27881; Mature: 27881

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKELLKILREKKPLVHHITNVVTVNDCANITLAIGALPVMAHALEEVEEMVSAADALVL
CHHHHHHHHHHCCHHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHEE
NIGTLTNEQVEAMIKAGKAANRFKVPVILDPVGAGATKLRTQSSKKILEEVKISVIKGNS
EECCCCHHHHHHHHHHCCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHHEEEEECCC
AEISILAGKGGKIRGVESQGGVDDIAEAAKDLANAYNVVVAMSGATDIITDGKRIAYVKN
CEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCEEEEEEC
GHPMMGTITGTGCMLTSVVASFCGVCEDYFETTIEAFVAFGIAGERAAQSSNVKGPGSFK
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEE
VTFFDEIYNLTPEIIEKDKKVEV
EEEEHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure
MKKELLKILREKKPLVHHITNVVTVNDCANITLAIGALPVMAHALEEVEEMVSAADALVL
CHHHHHHHHHHCCHHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHEE
NIGTLTNEQVEAMIKAGKAANRFKVPVILDPVGAGATKLRTQSSKKILEEVKISVIKGNS
EECCCCHHHHHHHHHHCCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHHEEEEECCC
AEISILAGKGGKIRGVESQGGVDDIAEAAKDLANAYNVVVAMSGATDIITDGKRIAYVKN
CEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCEEEEEEC
GHPMMGTITGTGCMLTSVVASFCGVCEDYFETTIEAFVAFGIAGERAAQSSNVKGPGSFK
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEE
VTFFDEIYNLTPEIIEKDKKVEV
EEEEHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA